Cryptosporidiosis among Children with Diarrhea Admitted to Hospital Selayang and Hospital Sungai Buloh, Selangor, Malaysia

Abstract

Abstrac

Tthis study was conducted to verify the prevalence of cryptosporidiosis among children in Selangor.Consenting children aged 12 years and below, admitted with diarrhea to Hospital Selayang andHospital Sungai Buloh, were included as subjects. Each stool sample was divided into five aliquotsand tested for cryptosporidiosis using direct wet mount, Sheather’s sugar flotation, formalin-ethersedimentation, modified Ziehl-Neelsen staining, and direct monoclonal fluorescent antibody test.Of 130 stool samples, 6 (4.62%) were positive for Cryptosporidium spp. All children positive forcryptosporidiosis were aged < 4 years. Direct wet mount, formalin-ether sedimentation concentration,modified Ziehl-Neelsen staining, and direct monoclonal fluorescent antibody testing showed thesame number of positive samples (4.62%); Sheather’s sugar flotation detected one less positive sample(3.85%).

Keywords: cryptosporidiosi; children; diarrhea; hospital; Malaysia