A Note on Laboratory Colonization of Toxorhynchites splendens by Using an Artificial Mating Technique and Autogenous Aedes togoi Larva as Prey

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Wej Choochote
Atchariya Jitpakdi
Teerayut Suntaravitun
Anuluck Junkum
Kanisa Rongsriyam
Udom Chaithong



An alternative method for the colonization of Toxorhynchites splendens was established successfully by using artificial mating for adults and autogenous Aedes togoi larva as prey. This current method of rearing could enable a reduction in the large size cage (60 x 60 x 75 cm) for adult rearing and the small laboratory animals as a source of bloodmeal that are used to maintain the colonies of blood-fed prey, eg, Ae. aegypti, Ae. albopictus and Culex quinquefasciatus. The suitable age of adult males for artificial mating ranged from 3-6 days, with 65-90% and 100% mating and insemination rates, respectively. The average number of autogenous Ae. togoi larvae consumed by associated stages of Tx. splendens were 8.55 ± 2.74, 9.75 ± 3.86, 27.10 ± 7.89 and 61.30 ± 10.62 larvae in stages 1, 2, 3 and 4, respectively.

Keywords : Toxorhynchites splendens, colonization, Aedes togoi

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Research Note