Prevalence and risk factors of Babesia infections in cattle trespassing natural forest areas in Salakpra Wildlife Sanctuary, Kanchanaburi Province

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Keywords:
Prevalence B. bovis B. bigemina cattle PCR Salakpra Wildlife Sanctuary

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Patcharathorn Simking
1 Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand; 2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900,Thailand; 3 Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900 Thailand” (CASAF, NRU-KU, Thailand); 4 Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand
Nantawan Yatbantoong
Department of Large animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140
Nantiya Saetiew
1 Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand; 2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900,Thailand; 3 Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900 Thailand” (CASAF, NRU-KU, Thailand); 4 Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand;
Sinsamut Saengow
Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand
Wanisa Yodsri
Geo-informatics Service and Application Division, Bangkok, 10900, Thailand
Rattanawat Chaiyarat
Wildlife and Plant Research Center, Faculty of Environment and Resource Studies, Mahidol University, Thailand 73170
Sirichai Wongnarkpet
Department of Veterinary Diagnostic Services, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
Sathaporn Jittapalapong
Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand

Abstract

Bovine babesiosis is a major tick – borne disease in livestock development since this disease has  an effect on animal health led to economic losses due to increase mortality and lower production.  Babesiosis in cattle is caused by Babesia bovis and B. bigemina and transmitted by tropical cattle tick, Rhipicephalus (Boophilus) microplus. The objective of this study was to investigate the prevalence of Babesia infection in the cattle invading natural forest area of Salakpra Wildlife Sanctuary (SWS) in Kanchanaburi province using molecular assays. A total of 244 cattle blood samples were collected from SWS areas located among 3 districts of Kanchanaburi Province including Mueang, Si Sawat, and Bo Phloi and examined by polymerase chain reaction (PCR) using SBP2 gene to identify B. bovis and rap – 1α gene for B. bigemina. Factors associated with Babesia infection including location, age, sex, herd size, seasonal management, insect abundant, forage density, deworming, domestic pet in household, tick infestations in cattle and barn, and the grazing area management was statistically analyzed. The overall infection of Babesia spp. of cattle in SWS was 46.7% (114/244), comprising B. bovis (5.3%, 13/244), B. bigemina (38.9%, 95/244) and mixed (B. bovis and B. bigemina) (2.4%, 6/244). Mueang District had the highest infection at 47.9% (45/94). The potential significant factors associated with Babesia infections were herd size and age (p<0.05). The high infection of Babesia spp. in SWS indicated the possible risk of the wild to get infections via ticks contaminated in this areas.

Article Details

Issue
Vol. 37 No. 1: June 2014
Section
Original Articles