@article{Romphruk_Puapairoj_Romphruk_Leelayuwat_2014, title={HLA-B*1502 typing kit by one step PCR}, volume={29}, url={https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/23723}, abstractNote={<p><strong><span style="text-decoration: underline;">Background and Objective</span></strong>: Human Leukocyte Antigens (HLA) is a group of antigens expressed on most nucleated cells. Their functions are involved in immune responses. There are several groups reported the associations between the HLA genes with the hypersensitivity to some drugs, such as carbamazepine (CBZ), particularly <em>HLA-B*1502</em> with the Stevens-Johnson syndrome (SJS) or cutaneous form of toxic epidermal necrosis (TEN). Therefore, screening for the presence of <em>HLA-B*1502</em> in patients before CBZ and phenytoin (PHT) treatment is an alternative to prevent such adverse events.  To detect <em>HLA-B*1502</em> by commercial typing kit is very expensive and requires special equipments. In this study, a high resolution PCR-sequence specific primers (PCR-SSP) technique has been developed and evaluated to define <em>HLA-B*1502</em>.</p><p><strong><span style="text-decoration: underline;">Method</span></strong><span style="text-decoration: underline;">:</span>  The <em>HLA-B*1502</em> typing kit was developed upon the principle of PCR- SSP and multiplex- PCR. Twelve PCR reactions were amplified in one step PCR. The kit has been tested with 64 known standard DNA samples carrying <em>HLA-B*1502</em> by sequence based typing and 156 samples with known <em>HLA-B*15</em> by a low resolution PCR-SSP and another 100 blind samples.</p><p><strong><span style="text-decoration: underline;">Result:</span></strong> The sensitivity, specificity and accuracy of this kit is 100%.  Furthermore, this kit can define <em>HLA-B*1502 </em>with<em> </em>other<em> HLA-B*15 </em>subtypes or <em>HLA-B*1502 </em>with<em> HLA-B*46</em> or <em>HLA-B*1502 </em>with<em> </em>other <em>HLA-B* </em>alleles. The turnaround time for the typing is 2.5 hours after DNA extraction.</p><p><strong><span style="text-decoration: underline;">Conclusion</span></strong><span style="text-decoration: underline;">:</span>  This study established a simple typing kit for <em>HLA- B*1502</em> detection with high efficiency and low cost. It can be used for screening in the patients with a risk of developing acute CBZ-induced SJS/TEN in general hospital laboratories.<ins cite="mailto:Khon%20Kaen%20University" datetime="2014-08-27T13:08"></ins></p>}, number={4}, journal={Srinagarind Medical Journal}, author={Romphruk, Amornrat and Puapairoj, Chintana and Romphruk, Arunrat and Leelayuwat, Chanvit}, year={2014}, month={Nov.}, pages={104} }