TY - JOUR AU - Dokduang, Hasaya AU - Loilome, Watcharin AU - Namwat, Nisana AU - Techasen, Anchalee AU - Pairojkul, Chawalit AU - Khuntikeo, Narong AU - Murakami, Yoshinori AU - Yongvanit, Puangrat PY - 2013/11/27 Y2 - 2024/03/29 TI - Inhibitory Effects of Xanthohumol on STAT3 Activation and Cancer Development in Cholangiocarcinoma Xenograft Model JF - Srinagarind Medical Journal JA - SRIMEDJ VL - 28 IS - 4 SE - Abstract of Interesting DO - UR - https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/14849 SP - 195 AB - <p><strong><span style="text-decoration: underline;">Background and objective:</span></strong><strong> </strong>STAT (Signal Transducer and Activator of Transcription) is a family of protein kinase and compose of seven members which play crucial roles in inflammation, immune response and cell development. However, constitutive activation of STATs is implicated in several cancers including cholangiocarcinoma (CCA). Our preliminary results revealed the implication of STAT3 in inflammation associated CCA and using Xanthohumol (XN), a chemopreventive agent extract from hops (<em>Humulus lupulus</em> L.), could inhibit STAT3 activation, CCA cell proliferation and induced CCA cell apoptosis. In this study, we aim to explore inhibitory effects of XN on STAT3 activation as well as CCA development in mouse xenograft model.</p> <p><strong><span style="text-decoration: underline;">Methods:</span></strong> Six- week- old female BALB/cAJ cl- nu/nu mice were subcutaneously injected with 2x10<sup>6</sup> cells of KKU- M214 at both flank sides. One week after tumors were visible, animals were divided into two groups; the control group was provided with a vehicle (0.5% ethanol) whereas treatment groups were administrated 50 µM and 100 µM of XN in drinking water for 30 days.Then, tumor volume, STAT3 activation as well as CCA development were identified.</p> <p><strong><span style="text-decoration: underline;">Results:</span></strong><strong> </strong><em>In vivo</em> oral administration of XN (50 µM and 100 µM in drinking water) for 30 days, significantly attenuated tumor growth in CCA inoculated mice (p &lt;0.01) without noticeable side effects. Molecular analyses showed that XN also inhibits STAT3 activation and tumor cell proliferation in animal model.</p> <p><strong><span style="text-decoration: underline;">Conclusions:</span></strong> Our findings reveal that XN exert therapeutic potential against CCA through inhibiting STAT3 both <em>in vitro</em> and <em>in vivo</em>, suggesting STAT3 as a promising target of XN for CCA treatment.</p> ER -