TY - JOUR AU - Proungvitaya, Siriporn AU - Khatkham, Natchanok AU - Thinglan, Chakkarin AU - Hong Ard, Papawadee AU - Changtrakun, Duangrudee AU - Promdee, Limthong AU - Proungvitaya, Tanakorn PY - 2013/11/27 Y2 - 2024/03/29 TI - Comparison of Creatinine Levels Determined by Jaffe kinetic and Enzymatic Methods JF - Srinagarind Medical Journal JA - SRIMEDJ VL - 28 IS - 4 SE - Abstract of Interesting DO - UR - https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/14866 SP - 208 AB - <p><strong><span style="text-decoration: underline;">Background and Objectives :</span></strong> Measurement of serum creatinine is important for evaluating of kidney function. Several methods have been using for determining of serum creatinine. Jaffe kinetic is the most widely use method for determining of serum creatinine. However, this method gives rather high measure level due to some interfering substances. Therefore, we aim to compare the serum creatinine levels determined by Jaffe kinetic method using in-house reagent to a highly sensitive enzymatic method used in Srinagarind Hospital.</p> <p><strong><span style="text-decoration: underline;">Methods :</span></strong> One hundred and fifteen sera were collected and measured for creatinine levels using enzymatic method from Srinagarind Hospital. Estimated glomerular filtration rate (eGFR) of each sample was calculated. The samples were divided into 5 five groups of 23 each base upon eGFR &lt;15, 15-29, 30-59, 60-89 and ≥90 mL/min, respectively. After that all samples were further measured for creatinine levels by Jaffe kinetic method.</p> <p><strong><span style="text-decoration: underline;">Results :</span></strong> The concordance of creatinine levels between the two methods was found in the group with eGFR of &lt;15 and 15-29 mL/min. By contrast, the disagreement of the two methods with significant difference (p-value &lt;0.05) was also found in the group with eGFR of 30-59, 60-89 and ≥90 mL/min, respectively.</p> <p><strong><span style="text-decoration: underline;">Conclusions :</span></strong> As a result, care must be taken in interpreting the result when serum creatinine level is measured by different principles.</p> ER -