TY - JOUR AU - Sangartit, Weerapon AU - Donpunha, Wanida AU - Kukongviriyapan, Upa AU - Pakdeechote, Poungrat AU - Kukongviriyapan, Veerapol AU - Surawattanawan, Praphassorn PY - 2013/11/27 Y2 - 2024/03/29 TI - Effect of Combined Tetrahydrocurcumin and Deferiprone on Oxidative Stress and Vascular Dysfunction in Iron Overloaded Mice JF - Srinagarind Medical Journal JA - SRIMEDJ VL - 28 IS - 4 SE - Proceeding DO - UR - https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/14891 SP - 271-275 AB - <p><strong><span style="text-decoration: underline;">Background and Objective:</span></strong><strong> </strong>Iron overload is an excessive accumulation of iron in the body. This condition can generate reactive oxygen species (ROS), which leads to oxidative stress and subsequently vascular dysfunction via the mechanism of reducing nitric oxide (NO), a key regulator of vascular homeostasis. Deferiprone (or L1) is used to prevent iron toxicity by chelating labile iron. Moreover, several studies indicated that tetrahydrocurcumin (THU) possesses strong antioxidant and vasculoprotective effect in various stress conditions. The present study was aimed to investigate whether supplementation with L1 plus THU can mitigate oxidative stress and vascular dysfunction in iron overloaded mice, a model represented iron overload condition.<strong></strong></p> <p><strong><span style="text-decoration: underline;">Methods:</span></strong><strong> </strong>Iron sucrose (10 mg/kg/day, i.p.) was injected to  Imprinting Control Regio (ICR) mice for 8 weeks<strong>.</strong> . L1 or THU at dose of 50 mg/kg/day was intragastrically administered through the period of iron overload induction.<strong> </strong>Blood pressure, vascular responsiveness to various vasoactive agents, lipid peroxidation markers were measured. The thoracic aortas were excised for assessment of superoxide radical (O<sub>2</sub><sup>·</sup><sup>-</sup>) production.</p> <p><strong><span style="text-decoration: underline;">Results:</span></strong> Iron overloaded mice exhibited high arterial blood pressure and impaired vascular responses to vasoconstrictor and vasodilators, Increase in O<sub>2</sub><sup>·</sup><sup>-</sup> production in vascular tissues and plasma malondialdehyde (MDA) concentration were found in iron overloaded mice. Treatment with L1 or THU partially alleviated these deleterious effects. Interestingly, combined therapy with L1 and THU exerted a greater effect than L1 or THU monotherapy.</p> <p><strong><span style="text-decoration: underline;">Conclusion:</span></strong><strong> </strong>Iron overload-induced by iron sucrose enhanced oxidative stress and vascular dysfunction in mice. L1 plus THU restored the blood pressure, decreased oxidative stress, attenuated vascular dysfunction.</p> <p><strong><span style="text-decoration: underline;">Key words</span></strong><span style="text-decoration: underline;">:</span> Iron overload, Oxidative stress, Vascular dysfunction, Deferiprone, Tetrahydrocurcumin</p> ER -