TY - JOUR AU - Pipattanaboon, Chonlatip AU - Natsrita, Piyathida AU - Boonha, Khwanchit AU - Benjathummarak, Surachet AU - Pitaksajjakul, Pannamthip AU - Ramasoota, Pongrama PY - 2021/08/20 Y2 - 2024/03/29 TI - Production of Truncated Recombinant Dengue Virus Envelope Protein and its Immunogenicity in BALB/c Mice JF - Srinagarind Medical Journal JA - SRIMEDJ VL - 36 IS - 4 SE - Original Articles DO - UR - https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/251819 SP - 438-443 AB - <p><strong><u>Background and objective:</u></strong> Dengue is a major public health problem in tropical and subtropical countries. The four dengue virus serotypes can cause a wide range of mild to severe diseases. Many research and development efforts are ongoing to find a better effective and accessible version of vaccine, therapeutic agent, or diagnostic tool. Envelope (E) protein is a primary target for serologic diagnosis and immunization. This work aimed to express and characterize truncated E (rE74-118) protein of dengue virus serotype 2 (DENV-2) in both<em> in vitro</em> and <em>in vivo</em> properties.</p><p><strong><u>Methods and results:</u></strong> A truncated DENV-2 envelope E protein, amino acid sequence 74-118, encoding gene was amplified and cloned into the pET-32b plasmid. The recombinant plasmid was then expressed in <em>Escherichia coli</em> (SHuffle) to produce the recombinant protein rE74-118. Recombinant E protein in truncated form (rE74-118) was successfully constructed, expressed, and purified in the concentration of 5.8 mg/ml. The rE74-118 protein was tested for its specificity with an anti-E monoclonal antibodies and dengue patient sera.&nbsp; Furthermore, its immunogenicity in BALB/c mice was also tested. The results showed that the rE74-118 protein can specifically react to anti-E monoclonal antibodies and dengue patient sera. This protein also induces the humoral response with a low-level of neutralizing activity against DENV-2, as well as the protein do not show enhancing activities against all four serotypes.&nbsp;</p><p><strong><u>Conclusion:</u></strong> The truncated rE74-118 protein showed both antigenic and immunogenic properties in these <em>in vitro</em> and <em>in vivo</em> characterizations.</p> ER -