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Please note that Agriculture and Natural Resources (ANRES) will be fully functional under the ScholarOne system again from 1 April 2023 onward. Every manuscript is charged USD 200 per article after acceptance (THB 5,000 per article for Thai affiliation as ANRES is supported by Kasetsart University and the Thai government.) No refund is possible once the fee has been paid.</p> https://li01.tci-thaijo.org/index.php/anres/article/view/268608 Biophysical-based sustainable management of KHDTK Mungku Baru: Mapping conservation, research and utilization blocks 2025-07-31T11:33:57+07:00 Nanang Hanaf nananghanafi@umpr.ac.id Ahmad Jauhari nananghanafi@umpr.ac.id Gusti Muhammad Hatta nananghanafi@umpr.ac.id Hafizianor nananghanafi@umpr.ac.id <p>Importance of the work: Sustainable management of Special Purpose Forest Area <br>(KHDTK) Mungku Baru, requires area planning based on mapping and determining <br>management blocks that are in accordance with biophysical conditions.<br>Objectives: To create management plots based on watershed boundaries and group them <br>into management blocks based on biophysical conditions: vegetation density, land surface <br>temperature, carbon stock and land cover type.<br>Materials and Methods: Management plots were designed by considering watershed <br>boundaries using the Digital elevation model (DEM), normalized vegetation index (NDVI) <br>analysis and land surface temperature (LST) analysis based on Landsat 9 imagery, <br>carbon stock analysis and land cover analysis. Management blocks were created based on <br>a weighted overlay of management plots with NDVI, LST, carbon stock and land cover.<br>Results: The overlay of management plots, NDVI, LST, land cover and carbon stocks <br>produced 26 conservation blocks, with an additional 24 plots in the research and <br>development block, 6 plots in the indigenous cultural block and utilization and 6 plots in the <br>rehabilitation block. The minimum area limit for KHDTK utilization was no more than 10% <br>of the area, in accordance with applicable regulations.<br>Main finding: The arrangement of the KHDTK Mungku Baru area was designed by creating <br>management blocks according to biophysical conditions, with the aim being to ensure the <br>sustainability of the KHDTK Mungku Baru.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268615 Efficacy of bio-composted agricultural wastes and biocontrol agents in improving soil fertility and controlling dry root rot in King mandarin (Citrus nobilis) orchards 2025-07-31T13:40:51+07:00 Anh Thy Thi Chau ctathy@ctu.edu.vn Ngoc Thanh Nguyen ctathy@ctu.edu.vn Anh Thu Tat ctathy@ctu.edu.vn Guong Thi Vo ctathy@ctu.edu.vn <p>Importance of the work: Intensification of King mandarin cultivation has been <br>threatened by dry root rot (DRR) disease, causing unsustainable growth of this citrus fruit.<br>Objectives: To evaluate the efficiency of bio-fertilizers (BF) in combination with <br>different bio-control agents on improving soil properties and controlling DRR disease in <br>King mandarin orchards.<br>Materials and Methods: Rice straw waste material was composted with isolated fungi <br>from King mandarin orchards and rice fields. Five treatments were conducted in a <br>complete randomized design: 1): common farmer practice (the control); 2): recommended <br>inorganic fertilizers (RIF); 3): RIF plus BF inoculated with Trichoderma asperellum <br>(BF1); 4): RIF plus BF inoculated with Gongronella butleri (BF2); and 5): RIF plus BF <br>inoculated with Trichoderma sp. from a commercial source (BF3).<br>Results: BF amendment led to significant increases in soil organic matter, soil pH, labile <br>nitrogen, available phosphorus and exchangeable potassium compared to the control <br>with its high dose of inorganic fertilizers. There was a significantly lower density of <br>Fusarium solani and DRR suppression (40–82.5%) as result of BF amendment, with BF <br>plus isolated Trichoderma and Gongronella butleri being the most effective treatment. <br>Consequently, in the second year harvest, fruit yield increased by about 3.5 times that of <br>the control treatments (p &lt; 0.05).<br>Main finding: Using rice straw as waste material to make bio-compost with isolated <br>strains of native fungi could be an optimal practice for improving soil fertility and soil <br>suppression to reduce DRR and enhance fruit yield, promoting an eco-friendly system in <br>King mandarin orchards.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268617 Genomic comparison of gamma-aminobutyric acid-producing Levilactobacillus brevis and Companilactobacillus zhachilii strains from Thai fermented foods 2025-07-31T14:05:15+07:00 Amnat Pakdeeto amnat.p@chandra.ac.th Sukanya Phuengjayaem amnat.p@chandra.ac.th Engkarat Kingkaew amnat.p@chandra.ac.th Santhita Tungkajiwangkoon amnat.p@chandra.ac.th Chantharat Phitchayaphon amnat.p@chandra.ac.th Somboon Tanasupawat amnat.p@chandra.ac.th <p>Importance of the work: The GABA-producing Companilactobacillus zhachilii isolated from <br>fermented pork (‘nham’) demonstrates both efficacy and safety in GABA production.<br>Objectives: To identify the GABA-producing strains ASK-1 and ANB-1 to compare their genomes <br>with those of Levilactobacillus brevis and Companilactobacillus zhachilii strains.<br>Materials and Methods: Two fermented food samples were collected and used for the isolation of <br>lactic acid bacteria. The strains obtained were evaluated for GABA production. The selected strains <br>were identified based on their phenotypic and genotypic characteristics. Genomic comparisons of <br>the GABA-producing strains were performed using genome sequencing, assembly, annotation and <br>comparative analysis. This analysis included the examination of CRISPR regions, antimicrobial <br>genes, functional attributes and genome visualization.<br>Results: The GABA-producing lactic acid bacteria (ASK-1 and ANB-1) were isolated from <br>traditional Thai fermented foods (‘sai-krog-prieo’ and ‘nham’). The strains ASK-1 and <br>ANB-1 produced 9.12 ± 0.51 g/L GABA and 12.73 ± 0.57 g/L GABA, respectively. On the basis <br>of phenotypic characteristics and 16S rRNA gene sequencing, strain ASK-1 presented 100% <br>sequence similarity to Levilactobacillus brevis ATCC 14687T, whereas ANB-1 presented 99.93% <br>similarity to Companilactobacillus zhachilii HBUAS52074ᵀ. Genome analysis identified ASK-1 <br>as L. brevis ATCC 367, with an average nucleotide identity based on a BLAST (ANIb) value of <br>98.86% and ANB-1 with an ANIb value of 96.92%, to C. zhachilii HBUAS52074ᵀ. Additionally, <br>genomic studies revealed GABA-producing genes (gadA and gadB) via the KofamKOALA-KEGG <br>Orthology database.<br>Main finding: Levilactobacillus brevis ASK-1 and Companilactobacillus zhachilii ANB-1 efficiently <br>and safely produced GABA and exhibited probiotic properties, making them promising starter <br>cultures for functional food production in both local food and industrial fermentation processes.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University https://li01.tci-thaijo.org/index.php/anres/article/view/268621 Smartphone-based portable device for multi-sample colorimetric determination of nitrite in sausage 2025-07-31T14:45:07+07:00 Aekgphoonnada Yenuthok fscipsl@ku.ac.th Tharinee Saleepochn fscipsl@ku.ac.th Saijai Charnsethikul fscipsl@ku.ac.th Thitilapa Sae-Heng fscipsl@ku.ac.th Pongsak Lowmunkhong fscipsl@ku.ac.th <p>Importance of the work: A custom-designed light box with a hat-shaped cover ensured <br>consistent illumination for image analysis using a smartphone-based method.<br>Objectives: To develop a simple, rapid and accurate smartphone-based method for <br>detecting nitrite in sausages.<br>Materials and Methods: Samples were analyzed on a microplate and images were <br>captured using a smartphone camera. Green color intensity was measured and correlated <br>with the nitrite concentration. The method was validated against the Association of <br>Official Analytical Chemists (AOAC) standard, demonstrating high sensitivity and accuracy.<br>Results: Using the Griess reaction, the method formed an azo dye compound and provided <br>a portable, user-friendly and cost-effective alternative to conventional techniques. <br>A custom-designed photo box with LED lighting and a hat-shaped light fixture enabled <br>simultaneous analysis of multiple samples. The method provided excellent sensitivity <br>and accuracy, with a linear correlation between green color intensity and nitrite <br>concentration. The addition of 0.003% Brilliant Blue solution substantially expanded <br>the calibration curve’s linear range. Validation against the AOAC method confirmed <br>the reliability of the method, highlighting its potential for rapid, on-site food analysis.<br>Main finding: The custom-designed, hat-shaped light fixture effectively minimized <br>specular reflections from Light emitting-diode lighting, enabling the use of cost-effective <br>alternatives to iPads or tablets as illumination sources for simultaneous multi-sample <br>colorimetric measurements.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268622 Dynamics of coastal weather effects on Moler disease in Indonesia shallot production 2025-07-31T15:00:42+07:00 Natasha Belandina Cornelin Abolla unun@ugm.ac.id Junun Sartohadi junun@ugm.ac.id Muhammad Anggri Setiawan junun@ugm.ac.id <p>Importance of the work: Coastal microclimatic factors influence pathogen dynamics; <br>however, their impact on shallot diseases, such as Moler disease, remains poorly understood.<br>Objectives: To investigate multivariate relationships between coastal weather parameters <br>and Moler disease development in shallot production systems.<br>Materials and Methods: Field surveys were conducted across 15 sites in Parangtritis, <br>Yogyakarta, Indonesia, during the late rainy and dry seasons. Disease incidence and <br>infection rates were assessed weekly alongside six weather parameters monitored via <br>an automatic weather station. Principal component analysis (PCA) was used to analyze <br>weather-disease relationships.<br>Results: Disease incidence was significantly higher during late rainy season (28.50%) <br>compared to dry season (12.03%, p = 0.03), with infection rates of 0.14 units/d and <br>0.09 units/d (p &lt; 0.001). The PCA revealed 84.62% of the weather-diseases relationship <br>variance was explained by the two principal components. Radiation-precipitation <br>variables had strong positive associations with infection rates, while relative humidity <br>had an unexpected negative correlation (Pearson’s correlation coefficient r = -0.55). <br>Temperature had a secondary influence through weak through positive F2 loadings <br>(25.54%, r = 0.25). <br>Main finding: Coastal weather-disease interactions deviated from inland patterns, <br>particularly in humidity-disease relationships, necessitating maritime-specific disease <br>prediction models for effective management.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University https://li01.tci-thaijo.org/index.php/anres/article/view/268623 Insights into abdominal structures atozoal ultrastructure of coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) 2025-07-31T15:14:34+07:00 Pisit Poolprasert narrit.t@psu.ac.th Natthawut Charoenphon narrit.t@psu.ac.th Anan Kenthao narrit.t@psu.ac.th Peerapon Sornying narrit.t@psu.ac.th Narit Thaochan narrit.t@psu.ac.th <p>Importance of the work: The coffee berry borer, Hypothenemus hampei is an insect pest <br>of coffee worldwide; however, its abdominal ultrastructure remains undescribed, despite <br>its importance for informing targeted strategies to reduce its population and economic impact.<br>Objectives: To describe, using light and transmission electron microscopy (TEM), the <br>structure of the abdominal region of H. hampei with a focus on the ultrastructure of the <br>spermatozoa. <br>Materials and Methods: A sample of 20 mature male H. hampei, each with a total length <br>of 0.5–1.2 mm, was collected and processed using TEM. <br>Results: The epithelial ultrastructure of the midgut comprised enterocytes and <br>regenerative cells. Numerous mitochondria and lysosomes were observed together with <br>well-developed rough endoplasmic reticulum in the enterocytes. Abundant lipid droplets <br>and glycogen molecules were scattered in the cytoplasm of lipid cells, which were mostly <br>found close to the integument. Testicular maturation was associated with the appearance <br>of spermatids and spermatozoa. The elongated spermatozoon was composed of a head <br>that had two main layers (an acrosomal layer and a central nucleus), a midpiece of <br>elongated and twisted mitochondria and a tail with a typical 9 + 9 + 2 flagellar axoneme.<br>Main finding: The presence of uniflagellate and biflagellate forms not only sheds light on <br>insect evolution and phylogeny but also raises questions about their functional role in the <br>reproductive success of H. hampei.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University https://li01.tci-thaijo.org/index.php/anres/article/view/268625 Isolation of high-amylase Rhizopus arrhizus from Vietnamese ‘Banh men’ for efficient hydrolysis of glutinous rice (Oryza sativa var. glutinosa) 2025-07-31T15:36:30+07:00 Phuong-Mai Vo nptho@agu.edu.vn Duy-Tan Nguyen nptho@agu.edu.vn Huu-Thanh Nguyen nptho@agu.edu.vn Phu-Tho Nguyen nptho@agu.edu.vn <p>Importance of the work: Traditional fermented starters, such as ‘Banh men’, used in <br>the production of Vietnamese sweet-fermented rice are valuable sources of microbial <br>diversity with industrial potential. Identifying efficient amylase-producing molds from <br>these starters can enhance starch-based bioprocessing, while preserving traditional <br>fermentation knowledge.<br>Objectives: To isolate and characterize amylase-producing molds from Vietnamese Banh <br>men, assess their enzymatic activity and investigate their ability to hydrolyze glutinous <br>rice into fermentable sugars.<br>Materials and Methods: A sample of 14 mold strains was isolated from Banh men <br>and screened for amylase activity on starch agar. Top producers were identified <br>morphologically and via internal transcribed spacer sequencing. Selected strains were <br>cultivated in glutinous rice, with hydrolysis monitored based on the extract yield, <br>Brix value and sugar content (dinitrosalicylic acid method). <br>Results: Dominant isolates belonged to the Mucor and Rhizopus genera (70% prevalence). <br>The strains BMTS1 and BMCP2.3 (both Rhizopus arrhizus) had the highest amylase <br>activity (2.94 U/mL and 2.60 U/mL, respectively) and starch degradation indices (&gt;95%). <br>During rice hydrolysis, both strains produced approximately 100 mL of extract after 72 hr, <br>with reducing sugars increasing from 6.38 mg/mL to 37.49 mg/mL and polysaccharides <br>declining by 89%.<br>Main finding: R. arrhizus BMTS1 and BMCP2.3 from Banh men are potent starch<br>hydrolyzing agents, offering a sustainable solution for converting glutinous rice into <br>sugar-rich substrates for food and beverage applications.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268626 Evaluating efficacy of preoperative skin preparation with 2% chlorhexidine gluconate and 70% alcohol for reducing bacterial bioburden in dogs 2025-07-31T15:54:29+07:00 Nithida Boonwittaya taksaon.du@ku.th Chompoonek Yurayart taksaon.du@ku.th Saroch Kaewmanee taksaon.du@ku.th Taksaon Duangurai taksaon.du@ku.th <p>Importance of the work: Optimized antisepsis and protocols are essential for improving <br>veterinary surgical outcomes and preventing surgical site infections (SSIs).<br>Objective: To assess the effectiveness of 2% chlorhexidine gluconate and 70% alcohol <br>(CHG-Alc) in reducing the bacterial bioburden, including multidrug-resistant strains, <br>in canine surgical patients.<br>Materials and Methods: A sample of 10 client-owned dogs was involved in the proposed <br>standardized skin preparation protocol. Following an initial skin cleaning step, CHG-Alc <br>was sprayed onto the skin and left in contact for 3 min. Then, the excess solution was <br>gently removed. Bacterial cultures were collected at three time points: prior to antiseptic <br>application (the first), immediately after the 3-min contact period and removal of the <br>solution (the second) and 60 min after application (the third). Samples were processed <br>to quantify bacterial load, identify bacterial species and assess antimicrobial resistance.<br>Results: Pre-antisepsis, Staphylococcus pseudintermedius was the most frequently <br>isolated bacterium, including the multidrug-resistant strains. Bacterial counts decreased <br>significantly by over 99% at the second sampling time (p = 0.006), with complete absence <br>of growth (0 CFU/mL) at the third sampling time (p = 0.006).<br>Main Finding: CHG-Alc was highly effective for canine preoperative skin preparation, <br>significantly reducing bacterial counts and preventing reinfection. There should be strong <br>support for its use in veterinary preoperative protocols to minimize the risk of surgical <br>site infections.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268627 Optimizing prediction of key water quality parameters in tilapia river based cage culture system using simple parameters based on different deep learning models 2025-07-31T16:03:45+07:00 Roongparit Jongjaraunsuk ffiswrt@ku.ac.th Wara Taparhudee ffiswrt@ku.ac.th <p>Importance of the work: Deep learning (DL) models can predict key water quality <br>values in red tilapia culture using easily measured parameters. <br>Objectives: To enhance the efficiency and effectiveness of water quality monitoring by <br>developing a predictive DL model. <br>Materials and Methods: Convolutional neural network (CNN), long short‒term memory <br>(LSTM) and a hybrid CNN‒LSTM model were fine‒tuned using the Python software. <br>Model performance was assessed using root mean square error (RMSE), mean absolute <br>error (MAE), normalized root mean square error (NRMSE), Nash‒Sutcliffe efficiency <br>(NSE) and the coefficient of determination (R²). The results were statistically analyzed <br>based on a t test. <br>Results: During data collection, the mean ± SD values of water quality parameters were: <br>dissolved oxygen (DO), 4.03 ± 0.41 mg/L; water temperature (Temp), 27.63 ± 1.42°C; <br>water pH level (pH), 7.45 ± 0.11; total ammonia nitrogen (TAN) at 0.14 ± 0.04 mg/L; <br>nitrite‒nitrogen (NO2-‒N), 0.04 ± 0.05 mg/L; alkalinity (ALK), 105.41 ± 9.94 mg/L; <br>and water transparency (Trans), 75.31 ± 22.80 cm. The study evaluated the CNN, LSTM <br>and CNN‒LSTM models, with CNN‒LSTM consistently offering the best balance of <br>accuracy and processing speed. Specifically, it excelled at 1,000 epochs for DO and TAN <br>predictions and at 2,000 epochs for NO2-‒N and ALK predictions, with no significant <br>differences compared to observed values using standard measurement methods. <br>Main finding: The hybrid CNN‒LSTM model that used easily measurable water quality <br>parameters (Temp, pH and Trans), effectively predicted more difficult‒to‒measure <br>water quality parameters (DO, TAN, NO2-‒N and ALK). Additionally, the hybrid model <br>outperformed the individual CNN and LSTM models, providing better prediction <br>accuracy and faster processing times.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University. https://li01.tci-thaijo.org/index.php/anres/article/view/268633 Protective and therapeutic efficacy of mangosteen extract feed additive against Vibrio parahaemolyticus AHPND strain in Pacific white shrimp (Litopenaeus vannamei) 2025-08-01T14:08:26+07:00 Warawan Saengthamat jrojtinnakorn@hotmail.com Jumroensri Thawonsuwanb jrojtinnakorn@hotmail.com Ammara Sinprasertporn jrojtinnakorn@hotmail.com Rutch Khattiya jrojtinnakorn@hotmail.com Jiraporn Rojtinnakorn jrojtinnakorn@hotmail.com <p><strong><u>Importance of the work</u></strong>: Shrimp aquaculture has faced severe losses due to an epizootic bacterial disease caused by the <em>Vibrio parahaemolyticus</em> AHPND (VpAHPND) strain, characterized by pale-to-white color with atrophy of the hepatopancreas and migh mortality. This study highlights the potential of MEX1<sup>TM</sup>, a phytobiotic from mangosteen pericarp extract, as a natural, prophylactic feed additive to enhance health and AHPND resistance in Pacific white shrimp.</p> <p><strong><u>Objectives</u>:</strong> To assess the effect of 0.05% (weight per weight) MEX1<sup>TM</sup> supplement on growth, immune response and resistance to VpAHPND.</p> <p><strong><u>Materials and Methods</u></strong>: Juvenile shrimp were fed a MEX1<sup>TM</sup>-supplemented diet and performance was evaluated through growth indices, hematological profiles, enzyme activities and immersion-based AHPND challenge trials mimicking natural infection.</p> <p><strong><u>Results</u></strong>: MEX1<sup>TM</sup> significantly improved growth performance (% weight gain, Fulton's condition factor, average daily gain, specific growth rate, % feed efficiency and feed conversion ratio) and survival rate, which increased from 58.30% in controls to 91.42% in the MEX1™-treated group. The extract enhanced protein digestive enzymes (trypsin and chymotrypsin) and hematological parameters (total hemocyte count, differential hemocyte count and superoxide dismutase). In the VpAHPND resistance test, of the three experimental groups evaluated: controls, Tre-MEX (as the VpAHPND-treated group and Pre-MEX (as the VpAHPND-protected group), the Pre-MEX group had significantly elevated cellular immune parameters and the lowest pathogen loads in the hemolymph, hepatopancreas and intestine compared to both the Tre-MEX and control groups.</p> <p><strong><u>Main finding</u></strong>: VpAHPND primarily targeted and damaged hyalinocytes, while MEX1<sup>TM</sup> stimulated hematopoiesis, particularly granulocyte production, in response to infection. The mangosteen extract functioned as a growth promoter and an immunostimulant, boosting shrimp resilience against VpAHPND. These results supported the application of MEX1<sup>TM</sup> as a natural, effective feed additive for disease prevention and performance enhancement in shrimp aquaculture.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 Agriculture and Natural Resources https://li01.tci-thaijo.org/index.php/anres/article/view/268635 Assessing climate change impacts on meteorological drought indices for agricultural areas in Chi River Basin, Thailand: Comparative analysis of standardized precipitation index and standardized precipitation evapotranspiration index 2025-08-01T14:33:36+07:00 Thanasit Promping tawatchai@eng.src.ku.ac.th Chutipat Foyhirun tawatchai@eng.src.ku.ac.th Tawatchai Tingsanchali tawatchai@eng.src.ku.ac.th <p><strong>Importance of the work</strong><span style="font-weight: 400;">: Meteorological drought indices play a critical role in understanding the impact of climate change on water resources.</span></p> <p><strong>Objectives</strong><span style="font-weight: 400;">: To assess meteorological drought indices under future climate change impact in the Chi River Basin, a drought-prone region in northeast Thailand.</span></p> <p><strong>Materials and Methods</strong><span style="font-weight: 400;">: The responses to projected climate changes were elucidated for the widely used standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI). Projections indicated shifts in rainfall and temperature based on three regional climate models (ACCESS, CNRM and MPI) under two commonly used climate change representative concentration pathways (RCP4.5 and RCP8.5).</span></p> <p><strong>Results</strong><span style="font-weight: 400;">: Analysis of the SPI and SPEI values derived from computed monthly data for 1 mth, 3 mth and 6 mth drought intervals indicated a temporal shift in drought conditions. Specifically, SPI exhibited a dry-to-wet-to-dry pattern across the 2020s, 2050s, and 2080s, while SPEI showed a persistent wet condition in the 2020s and 2050s, followed by a shift to dry conditions in the 2080s. Strong positive correlations (Pearson’s correlation coefficient = 0.86–0.97) between SPI and SPEI were observed from the 1990s to the 2080s. The SPI signaled potential drought events across all agricultural areas, while the SPEI indicated unaffected or wet conditions. However, the SPEI had a slower response to changes in drought conditions compared to the SPI. Model performance for both the SPI and SPEI showed unsatisfactory alignment with observed data under RCP4.5 and RCP8.5 during 2011−2020.</span></p> <p><strong>Main finding</strong><span style="font-weight: 400;">: SPI and SPEI values can be used as components of a composite drought indicator, aligning with recommendations from the World Meteorological Organization and the Global Water Partnership in 2016 for a multi-indicator approach to provide a comprehensive representation of all drought types.</span></p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 https://li01.tci-thaijo.org/index.php/anres/article/view/268638 Characterization of indole-3-acetic acid-producing marine yeasts and their effect on tomato (Solanum lycopersicum L.) seedlings 2025-08-01T14:44:10+07:00 Chutima Kaewkrajay kchutima@aru.ac.th Tuankumari Kotabar kchutima@aru.ac.th Tida Dethoupb kchutima@aru.ac.th <p><strong><u>Importance of the work</u></strong>: The marine yeasts <em>Rhodotorula mucilaginosa</em> DMKU 7233-1 and <em>Papiliotrema laurentii</em> DMKUFC10-4 had strong potential for producing indole-3-acetic acid (IAA). Their cell suspension promoted tomato seed germination and increased stem size.</p> <p><strong><u>Objectives</u></strong>: To screen IAA-producing yeasts, analyze their phytobeneficial attributes, evaluate their antagonistic activity against <em>Agroathelia rolfsii</em>, to study low-cost substrates suitable for IAA production and to evaluate the effectiveness of yeast cells on tomato seed germination.</p> <p><strong><u>Materials and Methods</u></strong>: In total, 49 marine yeasts were screened for IAA production. Phytobeneficial attributes (nutrient solubilization, production of cell wall-degrading enzymes and synthesis of active substances) were evaluated. The antagonistic activity was studied between the yeasts and <em>A. rolfsii</em>. Low-cost carbon sources for IAA production were investigated and tomato seed bioassays were conducted.</p> <p><strong><u>Results</u></strong>: The marine yeasts <em>R. mucilaginosa</em> DMKU 7233-1 and <em>P. laurentii</em> DMKUFC10-4 produced the best results for IAA production. Although neither strain could solubilize phosphate or zinc oxide, and they did not produce siderophores or NH<sub>3</sub>, both strains were able to produce catalase and cellulase. There was no antagonistic activity between either of the yeast strains and <em>A. rolfsii</em>. IAA production in sugarcane molasses medium amended with 0.1% L-tryptophan (L-trp) yielded the best results at 1,379.4 ± 29.6 mg/L with DMKU 7233-1 and 681.4 ± 38.8 mg/L with DMKUFC10-4. Tomato seedlings treated with <em>P. laurentii</em> DMKUFC10-4 had a high germination rate (100%), while <em>R. mucilaginosa</em> DMKU 7233-1 produced the best outcome based on enhancement of stem size.</p> <p><strong><u>Main finding</u></strong>: The yeast strains DMKU 7233-1 and DMKUFC10-4 were effective at producing IAA in a medium amended with 0.1% L-trp. Sugarcane molasses was a suitable carbon source. Cell suspension of both yeast strains promoted the growth of tomato seedlings.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 https://li01.tci-thaijo.org/index.php/anres/article/view/268639 Probiotic properties and antibacterial activity against aquatic pathogens of non-starch, polysaccharide-degrading Bacillus velezensis newly isolated from termite (Termes comis) guts 2025-08-01T14:58:33+07:00 Kittipong Chanworawit fsciprd@ku.ac.th Putsawee Tomtong fsciprd@ku.ac.th Poramet Chuglum fsciprd@ku.ac.th Pinsurang Deevong fsciprd@ku.ac.th <p><strong><u>Importance of the work</u></strong>: Termite gut bacteria harboring non-starch polysaccharide (NSP) degradation and probiotic properties are beneficial as a microbial fermentation additive for plant-based animal feed.</p> <p><strong><u>Objectives</u></strong>: To evaluate the probiotic properties and antimicrobial activity against aquatic animal pathogens of NSP-degrading <em>Bacillus </em>sp. isolated from the guts of the termite <em>Termes comis.</em></p> <p><strong><u>Materials and Methods</u></strong>: NSP-degrading bacteria were isolated and screened from the guts of termites. The selected bacteria were identified molecularly and assayed for NSP-degrading activity, as well as being evaluated for their antimicrobial ability and probiotic properties (stress tolerance, antioxidant activity, bacterial safety profile and cell surface characteristics).</p> <p><strong><u>Results</u></strong>: Among the 49 bacterial isolates obtained from the termite gut samples, the isolates Tc10, Tc19 and Tc44 were selected based on the presence of NSP-degrading enzymes (cellulase, pectinase and xylanase). Based on 16S rRNA gene sequence analysis, these three isolates were closely related to <em>Paenibacillus lutimineralis</em> (Tc10), <em>Paenibacillus alvei</em> (Tc19) and <em>Bacillus velezensis</em> (Tc44). Among them, only <em>B. velezensis</em> Tc44 could survive in gastrointestinal tract conditions of pH 2.5 and 0.3% bile salt, exhibiting broad-spectrum inhibition against all five tested aquatic pathogenic bacteria. Furthermore, it had the highest antioxidant activity and presented NSP-degrading enzyme activity against all tested types, as well as being harmless based on hemolytic activity, biogenic amine production and antibiotic susceptibility. In addition, Tc44 had the highest adhesion capability to Caco-2 and HT-29 cells, with anti-adhesion ability against all the tested pathogens, based on competition, inhibition and displacement assays.</p> <p><strong><u>Main finding</u></strong>: Scientific knowledge was documented of NSP-degrading <em>Bacillus</em>-based probiotics obtained from gut samples of the soil-feeding <em>Termes comis</em>, which could be beneficial for nutritive improvement in animal feed and the prevention of aquatic animal disease in aquaculture.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 https://li01.tci-thaijo.org/index.php/anres/article/view/268640 Identification of water stress in tomato based on spectral indices and physiological parameters 2025-08-01T15:08:37+07:00 Jutiporn Thussagunpanit pariyanuj.c@ku.th Wanthanee Tewathammanaj pariyanuj.c@ku.th Phirada Thongyen pariyanuj.c@ku.th Benya Manochai pariyanuj.c@ku.th Pariyanuj Chulaka pariyanuj.c@ku.th <p><strong><u>Importance of the work</u></strong>: Generally, farmers use a large amount of water to produce greenhouse cherry tomatoes, resulting in increased production costs and possibly suboptimal excessive water use.</p> <p><strong><u>Objectives</u></strong>: To investigate the optimum water content for ‘Sweet Boy’ cherry tomato production, based on changes in tomato physiology and yield.</p> <p><strong><u>Materials and Methods</u></strong>: A completely randomized design was used, consisting of four levels of pot water capacity (PC; 60%PC, 80%PC, 100%PC (the control) and 120%PC) as the water-holding level in the growing medium. Physiological responses and tomato yield in each treatment were measured.</p> <p><strong><u>Results</u></strong>: Water levels of 60%PC and 80%PC decreased the tested spectral indices (the normalized difference vegetation index, the normalized difference red-edge index and the photochemical reflectance index). Furthermore, a water content of 60%PC significantly reduced the net photosynthetic rate (22.63–32.08%) and stomatal conductance (57.23–88.29%) in the vegetative and flowering stages compared to 100%PC. In addition, 100%PC achieved similar physiological and yield responses as 120%PC. After fruit harvest, 60%PC and 80%PC reduced the fruit weight and the total yield per plant. However, 60%PC produced significantly higher total soluble solids (9.48°Brix), ascorbic acid (9.11 mg/kg fresh weight, FW) and lycopene (4.24 mg/kg FW) contents than the control.</p> <p><strong><u>Main finding</u></strong>: Irrigation at 100%PC and 120%PC produced similar physiological responses and yields in cherry tomato plants. Therefore, 100%PC irrigation reduced water use by 32% compared to the 120%PC treatment while maintaining high yield and acceptable fruit quality. It is recommended that farmers irrigate at 100%PC to optimize productivity and efficient water use.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 https://li01.tci-thaijo.org/index.php/anres/article/view/268609 Anthocyanins in goat diets: A Review 2025-07-31T11:59:27+07:00 Narin Charoenphun narinch@buu.ac.th Supreena Srisaikham narinch@buu.ac.th Pramote Paengkoum narinch@buu.ac.th Juan Boo Liang narinch@buu.ac.th Tian Xingzhou narinch@buu.ac.th <p>Anthocyanins are common in fruits (especially berries), vegetables, grains and purple- or red-colored <br>flowers. They have antioxidant properties and thus play a role in preventing or reducing the risk of chronic <br>disorders such as cardiovascular disease, cancer and diabetes. Anthocyanin-containing plants included in <br>the diet of goats increased the anthocyanin content of milk, which adds value by providing functional <br>benefits for consumers’ health. In addition, they improved growth performance and meat quality <br>and prevented lipid oxidation by promoting the antioxidant status and elevating the proportions of <br>polyunsaturated fatty acids in the muscle or superoxide dismutase activity. However, anthocyanin <br>concentration is plant type-specific. Therefore, it is necessary to further investigate the nutritional values <br>and anthocyanin levels of plant materials for application in the diets of ruminants, including goats, and <br>anthocyanin persistence in the fresh and processed milk or meat.<br>Importance of the work: Anthocyanin-rich plants have considerable potential for use as natural <br>antioxidants in meat or dairy goat diets.<br>Objectives: To gather insights into the role of anthocyanins in goat nutrition, contributing to future research <br>and sustainable goat production.<br>Materials and Methods: A systematic approach was adopted, including defining the scope, searching <br>appropriate databases and keywords, selecting reliable and up-to-date references, collecting and organizing <br>data in a structured manner and synthesizing the collected data.<br>Results: Anthocyanin is a pigment present in the flavonoid compounds commonly found in plants, <br>with antioxidant effects that are beneficial to ruminant animals and human health.<br>Anthocyanin-rich plants have considerable potential for use as natural antioxidants in meat or dairy <br>goat diets, since they can: 1) improve the proportions of polyunsaturated fatty acids in muscle; 2) reduce <br>oxidative stress; 3) reduce methanogenic bacteria and improve rumen fermentation; 4) enhance superoxide <br>dismutase; and 5) enhance the anthocyanin composition in the milk. <br>Main finding: The use of anthocyanin-rich plants in goat diets can have a positive impact on goat health <br>and increase production, enabling them to produce functional dairy products or meat that is beneficial <br>for consumer health.</p> 2025-08-21T00:00:00+07:00 Copyright (c) 2025 online 2452-316X print 2468-1458/Copyright © 2025. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/), production and hosting by Kasetsart University Research and Development Institute on behalf of Kasetsart University