Journal of Mahanakorn Veterinary Medicine

Development of Loop-mediated Isothermal Amplification for detection of Fowl Adenovirus serotype 2 causing Inclusion body Hepatitis

2024-04-23T11:53:53+07:00

Inclusion Body Hepatitis (IBH) is a disease with a high mortality rate in chicks, causing significant devastation to the poultry production industry worldwide. Thailand first encountered an outbreak of IBH in 2007, caused by the fowl adenovirus serotype 2 (FAdV-2). To detect this virus, the study developed a loop-mediated isothermal amplification (LAMP) method using four primers specific to the hexon gene. The optimal results were obtained at a temperature of 63 degrees Celsius for 60 minutes. These results can be interpreted with the naked eye due to the addition of SYBR Green I in reaction mixture, and no reactions were detected with the genetic material of others viruses such as chicken anemia virus (CAV), Newcastle disease virus (NDV), duck plaque virus (DPV), infectious bronchitis virus (IBV), and egg drop syndrome virus (EDSV). The LAMP technique could detect virus levels as low as 10 copies/µl, making it 1000 times more sensitive than polymerase chain reaction (PCR) techniques. This study concludes that the LAMP technique is suitable for diagnosing IBH caused by the FAdV-2 virus. It is a simple, convenient, and fast method that does not require expensive equipment for testing and interpreting results. The technique has high specificity and sensitivity, making it ideal for fieldwork.

Preliminary study of hematological profile of buck without blood parasites detected

2024-05-23T19:30:22+07:00

The objective of this study was to investigate the hematology profile of buck from a farm in Nong Chok, Bangkok. 10 goat blood samples from external jugular vein in EDTA blood tubes were transferred to analyze at Animal Diagnostic Laboratory Center, Faculty of Veterinary Medicine, Mahanakorn University of Technology. The results showed that blood parasite was not found. Almost of hematological values were in normal range, but leukocytosis was found in 5 bucks, which had chronic inflammation and one had acute inflammation. Anemia was detected from five blood samples, suspected that these goats had a latent infection, which could make the animals for quarantine. The infection could be spread to other animals, or the infection is too low to detect by using the thin blood smear method. Therefore, PCR technique or blood biochemical test should be considered.

Spring viremia of carp disease

2024-03-20T13:48:26+07:00

SVC infections have been reported in a number of cyprinid fish species such as common carp, koi carp. Four strains of SVC have been reported include genogroup Ia, Ib, Ic and Id. Vector transmission could be occurred via carp louse (Argulus foliceus), leech (Piscicola geometra). Clinical signs revealed exopthalamos, petechial hemorrhage at skin, base of fin and cloaca. Histhopathological alteration showed oedematous perivasculitis and necotized hepatic tissue, hyperaemia and proliferation of reticuloendothelium and melanomacrophage centre in splenic tissue and pericarditis concurrent with myocardial degeneration. Detection of viral antigen, nucleic acid, virus isolation and identification was recommended for confirmatory diagnostic method. SVC disease control methods focused on avoiding exposure to the virus through farm hygienic practices, water treatment before fish cultivation.

Tetralogy of Fallot in an Alaskan malamute: a case report

2024-01-10T09:55:43+07:00

A 3-month-old female Alaskan malamute was presented with a grade IV/VI systolic heart murmur, exercise intolerance, and anorexia. On examination, the dog was found to have cyanosis and inadequate growth. Echocardiography revealed a dextroposition of the aorta, a large ventricular septal defect, severe pulmonic stenosis (maximal velocity 4.77 m/s), and right ventricular hypertrophy. The dog was diagnosed with tetralogy of Fallot (TOF) and managed medically with atenolol 0.3 mg/kg SID. The incidence of TOF in dogs has been reported to be approximately 0.6 - 1%. This report is the first to encompass a complete blood count, thoracic radiographs, and echocardiography findings in an Alaskan malamute diagnosed with TOF in Thailand.

Hepatoid gland adenomas on the dorsal proximal part of a dog tail

2024-02-19T09:18:29+07:00

A 4-year-old, 4.2 kg, Chihuahua, intact male dog visited the animal hospital due to the owner's concern over an over-lick behavior at the base of its tail that had continuously appeared for months. Physical examination at the base of the tail found change of skin color to purple-red bruise. The palpation showed a firm mass, 3*3*3 cm in size, located 5 cm from base of the tail. The histopathological examination results manifested as hepatoid gland adenoma, a rarely found tumor on the dorsal of base the tail. The veterinarian performed a successful surgical treatment with a good prognosis after surgery. The surgical wound appeared to be dry and healing smoothly. However, the dog should be monitored further because hepatoid gland adenoma has a 10–20 percent recurrence rate after surgery.