@article{Deesa_Thongsri_Chokchaisiri_Ganranoo_Thephinlap_Khoothiam_Suwannasom_Panya_Thepmalee_2021, title={Cytotoxicity against breast cancer cell line of 7R-acetylmelodorinol isolated from Xylopia pierrei Hance}, volume={14}, url={https://li01.tci-thaijo.org/index.php/journalup/article/view/247035}, abstractNote={<p>7<em>R</em>-acetylmelodorinol is extracted and purified compound from stem bark of <em>Xylopia pierrei</em> Hance. Previous studies, it was reported that 7<em>R</em>-acetylmelodorinol has the cytotoxicity activity against human small cell lung cancer (NCI-H187). However, the cytotoxicity activity against breast cancer is still unclear. The aim of the study is to determine cytotoxicity effect of 7<em>R</em>-acetylmelodorinol in breast cancer cell lines. The cell viabilities of MCF-7 breast cancer cell line after treated with various concentrations of 7<em>R</em>-acetylmelodorinol were evaluated by crystal violet staining, MTT assay, and clonogenic assay, respectively. For the crystal violet staining, treatment cells with 50 and 100 µM of 7<em>R</em>-acetylmelodorinol significantly decreased cell viability of breast cancer cells for 24 and 48 hours. The IC<sub>50</sub> of 7<em>R</em>-acetylmelodorinol for 24 and 48 hours in MCF-7 was 8.57±0.012 and 7.80±0.013 µM, respectively. The result of clonogenic assay revealed that colony formation showed a significant decrease in breast cancer cell treated with 7<em>R</em>-acetylmelodorinol at concentrations of 4 and 8 µM (<em>p</em>< 0.01). Taken together, 7<em>R</em>-acetylmelodorinol has cytotoxicity activity against breast cancer cells by three different methods. These findings suggested that 7<em>R</em>-acetylmelodorinol might be a useful compound for breast cancer therapy.</p>}, number={1}, journal={Health Science, Science and Technology Reviews}, author={Deesa , Natchalida and Thongsri , Ananda and Chokchaisiri, Ratchanaporn and Ganranoo, Lucksagoon and Thephinlap, Chonthida and Khoothiam, Krissana and Suwannasom, Nittiya and Panya, Aussara and Thepmalee, Chutamas}, year={2021}, month={Apr.}, pages={25–32} }