Rajamangala University of Technology Tawan-ok Research Journal

Optimizing the environment for the tropical climate

Sasitorn Srifuengfung — 2024-06-30

This research is an experimental research aims to find landscape design variables that affect the weather around the building. At the Micro-climate level that will make the weather cooler to get closer to thermal comfort zone. This research area studies are in front of the landscape technology building at Rajamangala University of Technology Tawan-Ok Bang Phra Campus Chonburi Province which is the location of the research area for Sustainable Solar Cabin Prototype Study Project. The research Study areas with the same climate and topography are 4 locations as following: 1. The area above the concrete yard 2. The area above the lawn. 3. The area with trees in a sparse forest style with a pond and 4. The area with trees in a dense forest style with a pond. The temperature and humidity were checked by installing the research tool are the UNI-T A12T temperature Humidity Meters, were installed at all 4 points above the area of use at a level of 1.40 meter above ground, 24 hours a day for 4 days. The research result found that the significant variables are 1. Materials. Concrete and grass surface Accumulates heat differently. 2. Shade: Areas in landscaping areas such as sparse forests and dense forests. The surface is under the shade of trees. Sunlight is blocked from the leaves. This causes the surface to not receive direct sunlight and 3. Increasing cooling from water evaporation (Evaporative cooling). The research conclusion is the coldest environment is the environment that is obscured by trees and sunlight falls on the surface. In this research, water evaporation was added to the study area. It was found that the influence of water evaporation was in the sparse forest landscaping area with not too dense foliage and near a pond. It was found that the temperature was cooler. The area is landscaped design with dense forest and dense foliage. and also found that The air temperature in the experimental building is 4 degrees lower than in the hottest part of the day. Due to the cooling influence that occurs, the temperature difference between outside and inside the experimental building is approximately 4 degrees. Such influence will cause the heat transfer to be 32.8% less than before, which may be concluded that the environment is manipulated. By increasing the evaporation of water Can make the temperature in this area drop closer to the comfort zone.

Landscape Architecture and Site Planning of Green Campus Faculty of Agriculture and Natural Resources Rajamangala University of Technology Tawan-Ok, Bo Thong District, Chonburi Province

Yiampol Choatpanyatum — 2024-06-30

This research is an extension of the study on "Geographic Information in Analyzing the Site Potential for Planning the Faculty of Agriculture and Natural Resources, Rajamangala University of Technology Tawan-Ok, Bo Thong District, Chonburi Province." The main objective is to design landscape architecture of Bo Thong institution as a Green Campus, utilizing experimental results, discussion outcomes, and overall recommendations. Additionally, it involves synthesizing the area to design a schematic layout and creating appropriate alternative or detail designs and construction for future development.

The analysis of developing the 660 Rai Bo Thong Institute in the hilly plain area with abundant water resources, dense communities, and accessibility to roads, infrastructure and communication, allows for the classification of three zones: 1. Public Zone, 2. Semi-public Zone serving as an educational and service center, and 3. Private zone include recreational and residential Zone. Furthermore, the usable areas are divided into eight zones, with two additional connecting points. These designs propose a green educational institution (Green campus) with ten areas known as Zones A-J, including 1) Academic Area, 2) Administrative Building Area, 3) Staff Residence -Dormitory Area, 4) Commercial Area, 5) Sports and Recreational Area, 6) Conservation Area, 7) Agriculture Area, 8) Service and Maintenance Area, 9) Plaza/Landmark Area, and 10) Green Walkway.

Summarizing the analysis of requirements, attitudes, university image, and the green university (Green campus) concept, the proposed educational institution layout for Bo Thong can be summarized as follows: 1) Space improvement planning, 2) Community involvement, 3) Development of renewable energy utilization, 4) Reduction of wasteful resource usage, reuse, and recycling, 5) Knowledge development for the community, 6) Landscape development for tourism, 7) Resource management, disaster prevention, and mitigation through creative development. The crucial aspects to consider for the green institution (Green campus) are energy and climate change, infrastructure planning, transportation, waste management, research, and water resources.

Regarding the design and layout, there are two options presented for utilizing the land in the green campus : 1. Using the existing roads as main thoroughfares, and 2. Using the existing roads as green walkways (Green Way) and moving the road north of the project.

The Design Distribution Centers and Agricultural Community Map the locality. Khao Jek Chuey Sao Hai, Saraburi Province

Ruchiwan Ansongkhram — 2024-06-30

This research aims to design a distribution center and a community map for local agricultural identity products: Jek Chuey Sao Hai rice (GI rice) in Saraburi Province. It also serves as a model for other communities as part of a research project on the development and enhancement of local agricultural identity products for sustainable community economic development : the Jek Chuey Sao Hai rice group (GI rice) in Saraburi Province. The research tools included questionnaires, interviews, surveys, analysis, and summarization to create guidelines for designing the distribution center and community map suited to the community and evaluate satisfaction. The findings revealed that consumers in Saraburi province became more aware and knowledgeable about local agricultural identity products spatially, and they expressed satisfaction with the development and design of the project. This was achieved by maximizing the benefits of local agricultural identity resources within the community. Additionally, there are recommendations for adopting the model to include sustainable energy-saving measurement processes, which can be extended to actual construction and use in the community.

Genetic variation of Mystus nemurus (Cuv. & Val.) in Lam Nam Kam by Random Amplified Polymorphic DNA (RAPD) Technique

Anawil Promthep — 2024-06-30

The purpose of this research was to assess genetic diversity of Yellow catfish (Mystus nemurus) (Cuv. & Val.) from five subpopulations including Sarasawadi Floodgate (A), Bang Nong Bueng Floodgate (B), Ban Na Khu Floodgate (C), Ban Nakham Floodgate (D), Lower Nam Kam Floodgate and flowing down to Mekong River at That Phanom district, Nakhon Phanom province (E). The genetic variation was done with nine primers (OPB-07, OPB-10, OPC-02, OPC-05, OPC-11, OPC-13, OPC-15, OPC-16 and UBC122). The size was between 200 – 2,500 and the total number of 97 DNA bands of 95 bands were found to be percent of polymorphic band between 46.39–81.44. The gene diversity (H) value was between 0.18–0.32. The value of gene differentiation (G_ST) and value of gene flow (N_m) overall populations in this study was 0.29 and 1.22, respectively. The genetic distance (D) values were found between 0.132-0.341. The UPGMA (Unweighted pair group method with arithmetic means) dendrogram clearly separated yellow catfish (Mystus nemurus) from Lum Nam Kam into two groups: the first group consisted of Sarasawadi Floodgate (A) and the second group consisted of Bang Nong Bueng Floodgate (B), Bang Na Khu Floodgate (C), Bang Nakham Floodgate (D) and Lower Num Khum Floodgate, flowing down to Mekong River at That Phanom district, Nakhon Phanom province (E). This research was very useful for breeding, management, and studying on the genetic structure of yellow catfish population in Lam Nam Kam in the future.

Effect of Plant Growth Regulators on Micropropagation of Nymphaea ‘Aurora’

Yaowamal Nammai — 2024-06-30

Water-lilies (Nymphaea spp.) are among the most popular aquatic plants due to their beautiful flowers, making them highly valued. The Nymphaea ‘Aurora’ is a hybrid hardy Water-lilies with small flowers that bloom profusely. Plant tissue culture technology has been employed in propagating plants to achieve desired characteristics and rapidly increase their numbers. This research aims to investigate appropriate sterilization methods for the rhizomes and young shoots. It also examines the effects of growth regulators on root induction and callus induction in aseptic conditions using MS (Murashige and Skoog, 1962) medium by semi-solid and liquid with temporary immersion bioreactor systems (TIBs). It was found that the most effective method for sterilizing the rhizome explants involved using 10 percent sodium hypochlorite solution for 20 minutes, followed by 0.2 percent mercuric chloride solution for 10 minutes. This method achieved a 100 percent sterility rate and an 86.67 percent survival rate. The shoots from the rhizome parts were able to induce shoots and leaves better than the young shoots. Tissue culture of the dwarf Water-lilies in liquid MS medium supplemented with 10 µM BAP (6-Benzylaminopurine) and 5 µM IAA (Indole-3-acetic acid) using a temporary immersion bioreactor system showed that shoot induction was higher than on semi-solid medium. This method produced the highest number of shoots and leaves, with 1.78 shoots and 14.00 leaves within six weeks. Regarding the effect of growth regulators on root induction, MS medium supplemented with 10 µM NAA (Naphthaleneacetic acid) induced the highest number of roots, with 16.22 roots. Concerning the effect of growth regulators on callus induction in young leaf, young shoot, and root cultures on semi-solid MS medium supplemented with 6 µM TDZ (Thidiazuron) and 0.5 µM NAA, it was found that the leaf and root tissues enlarged but did not develop into callus.

Product Development of Ravioli as substituted Water with Chaya Water Extract (Cnidoscolus aconitifolius)

Sineenart Suktanarak — 2024-06-30

The development of ravioli from Chaya water extract is this research. The objective of this study was to three standard formulas of ravioli and study optimal amount of chaya water extract which will be replace water in ravioli product. The standard formula was selected by sensory evaluation. The result found that most acceptable formula was formula one. This formula one was selected to study the effect of chaya water extract substituted water in the ravioli product. The ratio between water and chaya water extract at 5 different levels were 100:0, 75:25, 50:50, 25:75 and 0:100. The sensory evaluation found that the most acceptable the ratio between water and chaya water extract was 75:25. The 5 levels were analyzed to determine color and texture. The result found that the ratio of high value chaya water extract will affect the ravioli product. Ravioli product became green color and decreased hardness. Comparsion between raw flour and cooked flour of ravioli, the hardness and lightness of cooked flour were lower than raw flour of raviloi.

Identification of anti-Vibrio fungus (Penicillium decumbens) to inhibit the growth of Vibrio parahaemolyticus, a bacterial disease of shrimp

Junthip Tongjun — 2024-06-30

Fungal strain A5-1 was isolated from solar saltern land in Chanthaburi province and can inhibit Vibrio parahaemolyticus (Vp), a bacterial disease of shrimp by the dual culture technique. Morphology and molecular genetics identification using SSU rRNA and 18S rRNA gene sequences confirmed that the strain A5-1 is Penicillium decumbens that produces gelatinase, lipase, cellulase and protease, and showed an ability to grow in the presence of the different NaCl concentrations (0-60 psu) that classified as halotolerant fungi. 36 trial sets of factorial design is a method to determine the factors of anti-Vibrio substances production. All experiments showed the diameter of clear zone to inhibit Vp; therefore, the increasing of pH, ammonium concentrations and salinity have an effect on fungal sunstances production. The inhibition of maximum diameter is 2.56±0.06 cm showed in treatment T36 (1.0:9:30). The co-culture in broth presence the salinity of 10 and 20 psu, at 7.08x10⁵ and 1.36x10⁶ spores/mL of the fungal spore concentration can reduce number of Vp from 10⁵CFU/mL to less than or equal to 100 CFU/mL at 24 hours of incubation time, at 2.47x10⁶ and 2.94x10⁶ spores/mL of fungal spore concentration can reduce number of Vp from 10⁷CFU/mL to less than 100 CFU/mL at 96 hours of incubation time in 30 psu of salinity in broth. So, we can apply P. decumbens A5-1 to control number of Vp in shrimp culture.

Impact of organic fertilizer and weed control on weed density in pineapple crop

Nathaya Ruanpan — 2024-06-30

This study was condueted to evaluate the effect of different organic fertilizer applications and weed control on the diversity, density, and fresh weight of weeds in pineapple plantations during 90 days after planting. The experiment was conducted in Chonburi Province from November 2021 to February 2022. The randomized complete block design (RCBD) with three replications was used, and 12 treatments consisted of four organic fertilizer applications (control without using organic fertilizer, chopped pineapple leaves composted at 2.47 ton/rai, chicken manure at 1.67 ton/rai, and a mixture of chopped pineapple leaves composted at 2.47 and chicken manure at 1.67 ton/rai) with three weed controls (control without weeding, hand weeding once a month and using a mixture of bromacill at 320 and diuron at 320 g a.i./rai). Total ten weed species they are of emerged and growth on the studying field, five species were broad–leaved (50%), four grasses (40%), and one sedge (10%). Among various species, morning glory, horse purslane and nutgrass were the dominant species in all plot. Weed density and fresh weight were higher in morning glory and nutgrass with chopped pineapple leaves composted application, but lower in horse purslane than chicken manure application. Interestingly, using chopped pineapple leaves composted or chicken manure alone resulted in lower weed density and fresh weight than combining chopped pineapple leaves composted and chicken manure. The results indicated that 90 days after planting, the type and rate of organic fertilizer application affected weed density and fresh weight, which varied according to weed species. Weed control of horse purslane can use herbicides or hand weeding once a month, while morning glory and nutgrass use herbicides only. Suitable alternative of the use of organic fertilizers to improve fertility in pineapple cropping systems is chopped pineapple leaves composted because it recycling waste and reducing costs. This study lasted 30-90 days after planting and aims to effect on weeds only. However, long-term experiments on organic fertilizer application would help confirm the influence of organic fertilization on weed seed bank, weed density, species diversity, the growth and crop yield of pineapple for weed management in cropping systems to increase production potential and pineapple crop yield.

Design and Created Robot ABU 2022 “Conquest the Tower Cycle to into the Land of Bharat”

Patcharee Unmuang — 2024-06-30

This research paper aims to create a robot ABU 2022 that can perform the mission “Conquest the Tower Cycle to into the Land of Bharat” by applying an Arduino microcontroller board to control the mechanical system of the robot by receiving signals. The input from the Joy Stick play station PS2 wireless for Arduino is used to send signals to control motion motor drives and pneumatic controllers. with the following objectives: 1) To study and design the structure of robots. 2) To study and design a motor control system with an Arduino microcontroller board and 3) In order to design the robot in accordance with the rules and conditions of the competition. The operation of the robots consists of 1) the first robot has to destroy the Lagori Tower 2) the second robot has to throw a ball at Ball placed on the robot which is 1,300 mm. above the floor and has a distance of 3,500 mm. and 3) let the robot go to the lagori from the floor. and then placed on a 200 mm high base at least 1000 mm The results of the experiment show that the structure of the ABU 2022 robot was designed by using the Arduino Mega microcontroller board as a processing unit to control operations according to the commands of the PlayStation 2 joystick and send data to the Arduino Mega microcontroller board. to be processed according to commands for controlling the robot, with the control method being controlled by programming and the operation of the program into the Arduino Mega microcontroller board to allow R1 and R2 robots to work according to rules and regulations to control various control devices. To develop the benefits and increase the efficiency of studying and designing robot structures. To be able to carry out missions “Conquest the Tower Cycle to into the Land of Bharat”.

Production Methods of Ready-to-Use Fermented Vinegar Starter Culture

Khemmapas Treesuwan — 2024-06-30

Fermented vinegars are popular as healthy drinks due to their high nutritional value and ability to balance the various systems in the body. For example, reduces blood pressure, decreases the risk of cardiovascular disease, prevents infection and antivirus. However, the fermentation processes are complicated, and the procedures can take place only in the laboratories. Therefore, this study aimed to develop a ready-to-use vinegar starter culture for vinegar fermentation and convenient transportation.

In this study was conducted to develop dry starter culture for vinegar fermentation to facilitate production, transportation, and reduce microbial contamination. Raw materials were compared to the suitability of four substrates (rice flour, pineapple pulp, rice bran, and rice bran) for starter culture production. The additives that could reduce contamination in the production of dry starter culture for vinegar fermentation were also investigated. Moreover, the study investigated the optimum temperature and storage period for starter culture that could affect the efficiency of vinegar fermentation. Acetobacter aceti TISTR 354 was used in a semi-continuous fermentation process. The results showed that rice bran mixed with 95% ethanol and 0.2% propionic acid stored at 4 °C could reduce contamination and be stored for up to 5 weeks. From week 1 to 5, the average acetic acid production was 6.08% and 4.50%, respectively, which were significantly different (p < 0.05).