TY - JOUR AU - Win, Zin Nwe AU - Thanasansurapong, Sariyarach AU - Munyoo, Bamroong AU - Tuchinda, Patoomratana AU - Soodvilai, Sunhapas PY - 2022/11/01 Y2 - 2024/03/28 TI - Investigation on chemosensitizing effect of cycloartane triterpenoids isolated from Gardenia sessiliflora on non-small cell lung cancer JF - Science, Engineering and Health Studies JA - SEHS VL - 16 IS - SE - Health sciences DO - 10.14456/sehs.2022.29 UR - https://li01.tci-thaijo.org/index.php/sehs/article/view/254654 SP - 22050013 AB - <p>The mainstay treatment for non-small cell lung cancer (NSCLC) is chemotherapy. However, developing multidrug resistance to chemotherapy is still the main reason for relapse and poor clinical outcomes. P-glycoprotein (P-gp) is related to multidrug resistance and inhibitors of P-gp have been reported as chemosensitizers. Therefore, the aim of this present study was to evaluate the chemosensitizing effect of pure compounds isolated from <em>Gardenia sessiliflora</em> in NSCLC using the A549 cell line. The P-gp function was determined by measuring the intracellular accumulation of [<sup>3</sup>H]-digoxin or [<sup>3</sup>H]-paclitaxel, substrates of P-gp. Moreover, cell viability and cell death were evaluated by MTT and apoptosis assays, respectively. The screening results of eight pure compounds revealed that compound 8 showed the most significant increase in accumulation of [<sup>3</sup>H]-digoxin and [<sup>3</sup>H]-paclitaxel. Compound 8 at 10 µM did not affect cell viability, whereas paclitaxel significantly decreased cell viability. Interestingly, the combination of compound 8 and paclitaxel significantly decreased cell viability and demonstrated a greater increase in the apoptosis population of A549 cells than a single treatment of paclitaxel. The results of this study indicated that cycloartane triterpenoids compound 8 isolated from <em>Gardenia sessiliflora </em>increased the anti-cancer effect of paclitaxel in A549 cells via the inhibition of P-gp-mediated drug efflux.</p> ER -