Identification of the causal agents of cassava stem and root rot disease based on morphological characters and molecular data


  • Pornpimon Athipunyakom Department of Agriculture
  • Suneerat Seemadua กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร
  • Chanintorn Doungsa-ard กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร
  • Amonrat Kitjaideaw กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร



stem and root rot, dry root rot, black root rot, cassava, Phytophthora melonis, Sclerotium rolfsii, Neoscytalidium dimidiatum, Fusarium solani


Stem and root rot diseases were reported on cassava in Thailand. This study was
conducted to identify the causal agents of cassava stem and root rot, occurred in Muak Lek
district, Saraburi province; Khon Buri, Soeng Sang and Pak Chong districts, Nakhon
Ratchasima province and Aranyaprathet district, Sa Kaeo province. Twenty seven stem and
root rot specimens were collected from reported plantations from April 2015 to September
2016. The stem and root rot symptoms were classified into four groups namely, stem and
root rot, dried root rot, black root rot and root rot. The identification of causal agent of
stem and root rot disease was based on its morphological characteristics and molecular
data of ITS region. The ML phylogenetic reconstruction showed that the causal agent, of
stem and root rot disease was Phytophthora melonis. On the other hand identification
results of the causal agents of dried root rot, black root rot and root rot diseases based on
morphological data showed that they were Sclerotium rolfsii, Neoscytalidium dimidiatum and
Fusarium sp., respectively. The above mentioned fungi isolated from four symptoms had
been confirmed of their pathogenicity to cassava by Koch’s postulates procedure. The
isolated Phytophthora melonis, S. rolfsii, N. dimidiatum and Fusarium sp. were inoculated on
to cassava seedlings. It was found that the cassava seedlings inoculated with P. melonis,
S. rolfsii, N. dimidiatum showed disease symptoms after 15 days of inoculation. However,
cassava seedling inoculated with Fusarium sp. showed no symptom. We concluded that
P. melonis, S. rolfsii and N. dimidiatum were the causal agents of stem and root rot, dried
root rot and black root rot, respectively.


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How to Cite

Athipunyakom, P., Seemadua, S., Doungsa-ard, C., & Kitjaideaw, A. (2020). Identification of the causal agents of cassava stem and root rot disease based on morphological characters and molecular data. Thai Agricultural Research Journal, 37(3), 307–319.



Technical or research paper