Identification of the causal agents of cassava stem and root rot disease based on morphological characters and molecular data

Authors

  • Pornpimon Athipunyakom Department of Agriculture
  • Suneerat Seemadua กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร
  • Chanintorn Doungsa-ard กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร
  • Amonrat Kitjaideaw กลุ่มวิจัยโรคพืช สำานักวิจัยพัฒนาการอารักขาพืช กรมวิชาการเกษตร

DOI:

https://doi.org/10.14456/thaidoa-agres.2019.26

Keywords:

stem and root rot, dry root rot, black root rot, cassava, Phytophthora melonis, Sclerotium rolfsii, Neoscytalidium dimidiatum, Fusarium solani

Abstract

Stem and root rot diseases were reported on cassava in Thailand. This study was
conducted to identify the causal agents of cassava stem and root rot, occurred in Muak Lek
district, Saraburi province; Khon Buri, Soeng Sang and Pak Chong districts, Nakhon
Ratchasima province and Aranyaprathet district, Sa Kaeo province. Twenty seven stem and
root rot specimens were collected from reported plantations from April 2015 to September
2016. The stem and root rot symptoms were classified into four groups namely, stem and
root rot, dried root rot, black root rot and root rot. The identification of causal agent of
stem and root rot disease was based on its morphological characteristics and molecular
data of ITS region. The ML phylogenetic reconstruction showed that the causal agent, of
stem and root rot disease was Phytophthora melonis. On the other hand identification
results of the causal agents of dried root rot, black root rot and root rot diseases based on
morphological data showed that they were Sclerotium rolfsii, Neoscytalidium dimidiatum and
Fusarium sp., respectively. The above mentioned fungi isolated from four symptoms had
been confirmed of their pathogenicity to cassava by Koch’s postulates procedure. The
isolated Phytophthora melonis, S. rolfsii, N. dimidiatum and Fusarium sp. were inoculated on
to cassava seedlings. It was found that the cassava seedlings inoculated with P. melonis,
S. rolfsii, N. dimidiatum showed disease symptoms after 15 days of inoculation. However,
cassava seedling inoculated with Fusarium sp. showed no symptom. We concluded that
P. melonis, S. rolfsii and N. dimidiatum were the causal agents of stem and root rot, dried
root rot and black root rot, respectively.

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References

รังษี เจริญสถาพร ธีรนันท์ แซ่ลี้ อัมพร จุลคต ศักดา เชิดชู และฤทัยรัตน์ น้อยจาด. 2556. Phytophthora สาเหตุโรครากและหัวเน่าของมันสำปะหลังในประเทศไทย. หน้า 260-261. ใน: การประชุมอารักขาพืชแห่งชาติ ครั้งที่ 11 “อารักขาพืชไทย ก้าวไกลในประชาคมอาเชียน” 26-28 พฤศจิกายน 2556 ณ โรงแรมเซ็นทาราแอนด์คอนเวนชันเซนเตอร์ จังหวัดขอนแก่น.

สำนักงานเศรษฐกิจการเกษตร. 2558. สถานการณ์สินค้าเกษตรที่สําคัญและแนวโน้มปี 2559. สำนักงานเศรษฐกิจการเกษตร. กรุงเทพ. 241 หน้า.

Alvarez, Elizabeth; Llano R., Germán Alberto; Loke, John. 2002. Development of ecological practices to manage Phytophthora root rot of cassava (Manihot esculenta). Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 1 p. Retrieved on October 4, 2016 Available at:http://ciatlibrary.ciat.cgiar.org/articulos_ciat/phytophthora_root_rot_cassava_alvarez.pdf.

Athipunyakom P, S. Seemadua and C. Doungsa-ard. 2015. Diseases of dragon fruit in Thailand: Incidence and Management Strategies pp. 95-106. In: International Workshop on Improving Pitaya Production and Marketing, Frengshan, Kaohsiung, Taiwan, 7-9 September 2015.

Chuang MF, Ni HF, Yang HR, Hsu SL, Lai SY, Jiang YL.(2012) First report of stem canker disease of pitaya

(Hylocereus undatus, H. polyrhizus) caused by Neoscytalidi-um dimidiatum in Taiwan. Plant Dis 96:906

Charaensatapon R., T. Saelee, U. Chulkod and S. Cheadcoo. 2014. Phytophthora root and tuber rot of cassava in Thailland. pp. 66. In: The 5th Asian Conference on Plant Pathology (ACPP2014). 3-6 November. 2014. (Thai and Abstract in English)

Chuang, M.F., H.F. Ni, H.R. Yang, S.L. Shu, S.Y. Lai, Y.L. Jiang. 2012. First Report of Stem Canker

Disease of Pitaya (Hylocereus undatus and H. polyrhizus) Caused by Neoscytalidium

dimidiatum in Taiwan. Journal of Phytopathology 161 (11-12): 841-849.

Cooke, D.E.L., Drenth, A., Duncan, J.M., Wagels, G. and C.M. Brasier. 2000. A molecular phylogeny of Phytophthora and related Oomycetes. Fungal Genetics and Biology 30: 17-32.

Doyle, J.J.; Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, v.19, p.11-15.

Gallegly, M.E., C. Hong. 2008. Phytophthora: Identifying Species by Morphology and DNA Fingerprints. The American Phytopathological Society, St. Paul, Minnesota, U.S.A. 158 pp.

Ho, H.H. 1986. Phytophthora melonis and P. sinensis synonymous with P. drechsleri. Mycologia

: 907-912

Ho, H. H., Ann, P. J., and Chang, H. S. 1995. The genus Phytophthora in Taiwan. Inst. Bot. Acad. Sinica Monogr. Ser. 15

Ho. H. H., M.E. Gallegly and C.X. Hong. 2007. Redescription of Phytophthora melonis. Mycotaxon 102: 339-345.

Katoh, K., and Toh, H. 2008. Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics 9, 286-298.

Katsura, K. 1976. Two new species of Phytophthora causing damping-off of cucumber and trunk rot of chestnut. Transaction of the Mycological Society of Japan. 17: 238-242.

Leslie, J F. and B A. Summerell. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Ltd. 388 pp.

Machado, A.R., D.B. Pinho, S.A.S. Oiveira and O.L., Pereira. 2014. New occurrences of Botryosphaeriaceae causing black root rot of cassava in Brazil. Tropical Plant Pathology, V.39(6): 464-470.

Identification and Molecular Characterizations of Neoscytalidium dimidiatum Causing Stem Canker of Red-fleshed Dragon Fruit (Hylocereus polyrhizus) in Malaysia Masratul Hawa Mohd, Baharuddin Salleh and Latiffah Zakaria Identification and Molecular Characterizations of Neoscytalidium dimidiatum Causing Stem Canker of Red-fleshed Dragon Fruit (Hylocereus polyrhizus) in Malaysia

Masratul Hawa M., B. Salleh and L. Zakaria. 2013. Identification and Molecular Characterizations

of Neoscytalidium dimidiatum Causing Stem Canker of Red-fleshed Dragon Fruit

(Hylocereus polyrhizus) in Malaysia. Journal of Phytopathology 161(11-12): 841-849.

Oliveira, S.A.S, S.A.V. Boas, C.A.D. Braganca and J.de Oliveira. 2016. First report of Phytophthora

melonis causing cassava wilt and root rot in Bahia State, Brazil. Summa Phytopathol.,

Botucatu, V. 42, No. 1, p. 107.

Canu, B., Hunter, G.C., Linaldeddu, B.T., Franceschini, A., Maddau, L., Jung, T. and S. Denman. 2014. A taxonomic re-evaluation reveals that Phytophthora cinnamomi and P. cinnamomi var. parvispora are separate species. Forestry Pathology 44: 1-20.

Punja ZK.1985. The biology, ecology, and control of Sclerotium rolfsii. Ann Phytopathol 23:97–127.

Punja ZK and A. Damiani. 1996. Comparative growth, morphology, and physiology of three

Sclerotium species. Mycologia 88:694–706

Stamatakis, A. 2014. RaxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312-1313.

Stamatakis, A., Hoover, P. and J. Rougemont. 2008. A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57:758-71.

Sutton BC. 1980. The Coelomycetes, Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute, Kew, Surrey, England.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30:2725-2729.

Published

2020-02-23

How to Cite

Athipunyakom, P., Seemadua, S., Doungsa-ard, C., & Kitjaideaw, A. (2020). Identification of the causal agents of cassava stem and root rot disease based on morphological characters and molecular data. Thai Agricultural Research Journal, 37(3), 307–319. https://doi.org/10.14456/thaidoa-agres.2019.26

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Section

Technical or research paper