@article{Pruesapan_Kaewdoung_Wongwarat_2022, title={Study on Phylogenetic Relationships of Thai Ilex L. (Aquifoliaceae) Based on DNA Data}, volume={40}, url={https://li01.tci-thaijo.org/index.php/thaiagriculturalresearch/article/view/251377}, DOI={10.14456/thaidoa-agres.2022.1}, abstractNote={<p>Species boundaries among <em>Il</em>ex L. (Aquifoliaceae) are often not clear-cut due to the morphological overlap of related species. Genetic differentiation is thus needed to clarify their relationship. In the present study, we aimedto investigate the phylogenetic relationships of Thai Ilex species using DNA barcode technique. The DNA barcodes of <em>matK, rpl32-trnL, trnL-trnF</em> and ITS were examined and analyzed using Maximum Parsimony method. A total of 54 DNA sequence data obtained from this study representing 14 species of Thai <em>Ilex</em>. The phylogenetic trees resulted in five congruent main clades with morphological classification. Subgenus <em>Aquifolium</em> Section comprised <em>I</em>. odorata and<em> I</em>. <em>pubifructa </em>whereas the sectional placement for <em>I</em>. <em>depressifructu</em> was still unclear. Subgenus <em>Byronia</em> Section <em>Byronia</em> comprised I. <em>cymosa, I. wallichii, Ilex</em> sp. (1) andIlex sp. (2); Section <em>Lioprinus</em> comprised <em>I</em>. <em>phanganensis </em>and  <em>I</em>. <em>umbellulata;</em> Section <em>Paltoria</em> comprised only <em>I</em>. <em>triflora</em>. Subgenus Prinos Section Micrococca comprised only <em>I</em>. <em>micrococca</em>. The results confirmed species identification based on morphology. The placements in the phylogenetic trees supported grouping classification for <em>I</em>. <em>depressifructu</em>, <em>I</em>. <em>phanganensis</em> and <em>I</em>. <em>pubifructa</em> which were recently described as new species for Thailand and could be used as guideline for identifying the two unknown species. More representative species of Ilex and variable DNA barcodes should be added to resolve the indefinable information from the present study and to complete research on diversity of Aquifoliaceae in Thailand.</p>}, number={1}, journal={Thai Agricultural Research Journal}, author={Pruesapan, Kanchana and Kaewdoung, Anchalee and Wongwarat, Theerawut}, year={2022}, month={Jun.} }