การพัฒนาน้ำหมักชีวภาพผสมแบคทีเรียที่มีประโยชน์เพื่อส่งเสริมการเจริญเติบโตของพืชและควบคุมโรคขอบใบแห้งของข้าว

Main Article Content

พรภวิษย์ พงษ์สุภาพ
ชญานิศ กล่ำสุข
ดุสิต อธินุวัฒน์

Abstract

The aim of this research was to develop the fermented bio-extract combined with beneficial bacteria for enhancing rice plant growth and controlling of bacterial leaf blight of rice. Banana plant, kaffir lime, and chili were raw materials used in fermented bio-extract. Three fermented bio-extract were tested for Xanthomonas oryzae pv. oryzae. (Xoo) inhibition using a completely randomized design (CRD). The result revealed that banana trunk fermented bio-extract showed the efficacy to inhibit Xoo with causing clear zone of 1.56±0.26 cm. Efficiency of all 3 fermented bio-extract on enhancing rice plant performance was investigated at 7 days old plant. The results showed that the chili fermented bio-extract and banana trunk fermented bio-extract were the most efficiency treatment to promote growth of rice. Root length, shoot length, root fresh weight, root dry weight, shoot fresh weight and shoot dry weight were 6.02±0.83 and 5.35±0.37 cm, 5.5±0.48 and 5.2±0.26 cm, 2.52±0.45 and 1.97±0.16 g, 0.11±0.00 and 0.11±0.00 g, 2.96±0.05 and 2.67±0.17 g, and 0.20±0.01 and 0.19±0.01 g, respectively. From the previous results, banana stem fermented bio-extract was selected for further development. Antagonistic bacterial that showed phosphate solubilization, Bacillus sp. strain PSB3-5 was added into banana stem fermented bio-extract with 1x1011 cfu/ml final concentration and keep in room temperature (32±2 ºC) for 60 days. Results showed PSB3-5 populations were 6.4x109, 3.1x108, 3.6 x108, and 3.0x108 cfu/ml at 48 h, 15, 30, and 60 days after inoculated, respectively. These results indicating possibility of combined fermented bio-extract with phosphate solubilizing bacteria for the purpose of developing biological control method to reducing the use of synthetic fertilizer and pesticides.

Downloads

Download data is not yet available.

Article Details

Section
วิทยาศาสตร์ชีวภาพ

References

Andrea, G., Zavaglia E., Disalvo, A. Graciela, L. and De Antoni. 2000. Fatty acid composition and freeze-thaw resistance in Lactobacilli. J. Dairy Res. 67: 241-247.
Balouiri M., Sadiki M. and Ibnsouda SK. 2016. Methods for invitro evaluating antimicrobial activity: A review. J Pharm Ana. 6:71-9.
Brown, J. C. 1963. Annu. Rev. Plant Physiol. 14. 93.
Chaiyasut, C. 2010. Bio-extract. Bangkok: Thai Effect Studio Co.,Ltd. (in Thai)
Conor, F., Kimon, A., and Karatzas, G. 2012. Role of glutamate metabolism in bacterial responses towards acid and other stresses. J. Appl. Microbiol. 114: 11-24.
Dannis, W. and John, E. Cronan. 1997. Cyclopropne ring formation in membrane lipids of bacteria. Microbiology and Molecular Biology Reviews. 61: 429-441.
Department of Agriculture. 2004. Scientific Information Bio-extract (Part 1). Quickprint of Set Co.,Ltd. Bangkok, 51p. (in Thai)
Duncan, C. J., Bowler, K. and Davidson, T. F. 1970. Biochem. Pharmacol. 19. 2453.
Ehiowemwenguan, G., Emoghene, A.O. and. Inetianbor, J.E. 2014. Antibacterial and phytochemical analysis of Banana fruit peel. IOSR. J. Pharm. 4: 18-25.
Greenery. 2012. Development of Natural Agriculture to Sustainability Agriculture. Bangkok: The Publish. (in Thai)
Harris, C. M. and Livingstone, S. E. 1964. In Chelating Agents and Metals Chelates. (Dwyer, F. P. and Mellor, D. P., eds), p. 95. Academic Press, New York.
Heinz, G., Eugenia, P., Robert, A., Francesco, B., Guido, C., Daniela, B. and Markus, G. 2006. Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB. EMBO J. 25: 2643-2651.
Ian, R. Booth. 1985. Rugulation of cytoplasmic pH in bacteria. Microbiol. Rev. 49: 359-378.
Jeroen, W., Helene, F., Willem, M. de Vos., Oscar, P., and Tjakko, A. 2001. Cold shock proteins of Lactobacillus lactis MG1363 are involved in cryoprotection and in the production of cold- induced proteins. Appl. Environ. Microbiol. 67: 5171-5178.
Lana, S., and Tom, R. 2008. Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H+ and enhanced abiity to extrude H+. Res. Microbiol. 159: 458-461.
Ma, Y. and Marquis, R.E. 1997. Thermophysiology of Streptococcus mutans and related lactic-acid bacteria. Antonie Van Leeuwenhoek 70(2): 91-100.
Mahamud, R. 2005. Innovation in agricultural resource management for organic agriculture case study of organic rice farmers group, Amphoe Kudchum, Changwat Yasothon. Bangkok (Thailand): Kasetsart University, Bangkok (Thailand). Graduate School. (in Thai)
Mala, T. 2007. Organic Fertilizers and Biofertilizers: Production Techniques and Utilization. Faculty of Agriculture, Kasetsart University. Kasetsart University Press, Bangkok. (in Thai)
Marubodee, R. and W. Ruanpan. 2020. Effect of bioextract on growth and yield of arrowroot (Tacca Ieontopetaloides). RMUTSB Acad. J. 8(2): 153 - 164. (in Thai)
Rivera-Cruz, M., Trujillo, A., Córdova, G., Kohler, J., Caravaca, F. and Roldán, A. 2008. Poultry manure and banana waste are effective bio-fertilizer carriers for promoting plant growth and soil sustainability in banana crops. Soil Biology and Biochemistry. 40:3092-3095.
Ruchirasak, M. and P. Koetnoon. 2014. The Application of Fermented Bio-extract Produced from Egg of Golden Apple Snails (Pomacea canaliculata) to Enhance Yield of Rice. Princess of Naradhiwas University Journal 6(1): 73-80. (in Thai)
Sae-Ung, P and S. Meeklinhom. 2020. Biofertilizer Soaking Effect on Germination and Vigor of Rice Seed. Udon Thani Rajabhat University Journal of Science and Technology 8(1): 49-59. (in Thai)
Sangwanna, M., P. Seeniang and S. Patarapuwadol. 2018. Survey of bacterial blight disease and the need of knowledge in disease management in organic rice system in Roi Et province. Agricultural Science Journal, 49(3): 230–240. (in Thai)
Takehiro, M., Hidetake, E., Juzoh, U., and Tsuneo, H. 1997. Activity of H+-ATPase in ruminal bacteria with special reference to acid tolerance. Appl. Environ. Microbiol. 63: 2155-2158.
Terry, A., Raanan, A., Micheal, S., and Arthur, A. 1985. Buffering capacity of Bacilli that grow at different pH ranges. J. Bacteriol. Res. 162: 768-772.
Xia, C., Zhihong, S., He, M., and Heping Z. 2009. The acid tolerance association with expression of H+-ATPase in Lactobacillus casei. Int. J. Dairy Technol. 62: 272-276.