@article{บัวบาน_ศากยวงศ์_ธนานันต์_ธนานันต์_2020, place={Pathumthani, Thailand}, title={การประเมินความสัมพันธ์ทางพันธุกรรมของพืชครามสกุล Indigofera ด้วยลำดับนิวคลีโอไทด์ของยีน matK}, volume={9}, url={https://li01.tci-thaijo.org/index.php/tjst/article/view/247502}, DOI={10.14456/tjst.2020.60}, abstractNote={<p>The objective of this research was to sequence the nucleotides of <em>mat</em>K gene in order to identify 15 samples of <em>Indigofera </em>plant. This is a fast, timesaving, and low-cost method. When comparing nucleotide sequences with data in NCBI database, 3 species of <em>Indigofera</em>, which <em>are Indigofera tinctoria</em>, <em>Indigofera tinctoria</em> var. <em>arrecta,</em> <em>Indigofera caroliniana</em> and <em>Indigofera suffruticosa</em>, were identified<em>.</em> But it was not able to specify species for the sample no. 13. The data were analyzed with ClustalW and made a phylogenetic tree using UPGMA by MEGA version X program. It was found that the <em>Indigofera</em> samples were divided into 2 groups with genetic differences in a range of 0.105-0.389. Therefore, nucleotide sequences of other genes should be used for more efficiency. The information obtained from this research can be applied in the conservation and breeding of <em>Indigofera</em> plant<em>.</em></p>}, number={4}, journal={Thai Journal of Science and Technology}, author={บัวบาน ศุภรัตน์ and ศากยวงศ์ นิรมล and ธนานันต์ ธีระชัย and ธนานันต์ นฤมล}, year={2020}, month={Oct.}, pages={577–586} }