TY - JOUR AU - ปราบมาก, กนกนาฏ AU - จันทร, สุดาทิพย์ AU - คุ้มภัย, ภัทรพร PY - 2019/01/11 Y2 - 2024/03/29 TI - การศึกษารูปแบบลำดับนิวคลีโอไทด์บริเวณยีน 16S rRNA ของแบคทีเรีย Paenibacillus polymyxa โดยใช้ความแตกต่างของนิวคลีโอไทด์หนึ่งตำแหน่ง JF - Thai Journal of Science and Technology JA - Thai J. Sci. Technol. VL - 8 IS - 1 SE - วิทยาศาสตร์ชีวภาพ DO - 10.14456/tjst.2019.7 UR - https://li01.tci-thaijo.org/index.php/tjst/article/view/165785 SP - 66-76 AB - <p><strong>Abstract</strong></p><p><em>Paenibacillus polymyxa</em> plays a major role in the production of xylanase enzyme, which can be potentially applied in paper and animal feed industries. It is necessary to study the identification of the bacteria for preserving bacterial cultures and effectively bacterial utilization. In this study, we analyzed nucleotide sequences and determined the single nucleotide polymorphisms (SNPs) in <em>16S rRNA</em> gene of <em>P. polymyxa</em>. The results showed that 10 strains of <em>P. polymyxa</em> contained 11 to 14 <em>rRNA</em> gene copies of <em>16S rRNA</em> gene, which are located within each genome. The polymorphic sites were found among the multiple <em>16S rRNA</em> genes and the alignment results showed that 29 variable sites were detected. The region ranging from 465 to 488, which can be amplified using universal <em>16S rRNA</em> gene primers. This region could be divided into 2 nucleotide sequence patterns (pattern 1 and pattern 2). The pattern 1 could be used to identify <em>Paenibacillus</em> sp. BTK01 as <em>P. polymyxa</em> BTK01 that corresponded with the distance correlation coefficient (0.000 to 0.0017) of 10 reference strains. Thus, the patterns represented the variation within species, which could be used for discrimination of <em>P. polymyxa</em> at subspecies level. In conclusion, the results suggest that these 2 sequence patterns of <em>16S rRNA</em> gene may improve the <em>16S rRNA</em> database for species and subspecies identification of <em>P. polymyxa</em>.&nbsp;</p><p><strong>Keywords:</strong> <em>Paenibacillus; </em>SNP; <em>16S rRNA</em> gene; identification; subspecies</p> ER -