TY - JOUR AU - แก้วนาบอน, กาพย์แก้ว AU - เตชะศีลพิทักษ์, ธัญญะ AU - จารุวัฒนพันธ์, ทัศไนย AU - จันจุฬา, ณัฐพงค์ AU - บาลลา, นุชรัฐ PY - 2019/03/02 Y2 - 2024/03/29 TI - การชักนำให้เกิดแคลลัสจากชิ้นส่วนต่าง ๆ ของต้นลินเดอร์เนียในสภาพปลอดเชื้อ JF - Thai Journal of Science and Technology JA - Thai J. Sci. Technol. VL - 8 IS - 2 SE - วิทยาศาสตร์ชีวภาพ DO - 10.14456/tjst.2019.23 UR - https://li01.tci-thaijo.org/index.php/tjst/article/view/175575 SP - 138-145 AB - <p><strong>Abstract</strong></p><p>Lindernia is a flowering plant with high genetic diversity. This plant can survive in almost any environment and flowers all year round, making it to be suitable for improving as an ornamental plant for the future. In plant regeneration, BA and NAA are useful for shoot and internode inductions. The aim of this research was to study the regeneration potential of Lindernia explants (cotyledon, leaf, and internode) on ½ MS media with BA at concentrations ranging from 0.5-2.0 mg/L) in <em>in vitro</em> culture. Callus sizes and shoots were recorded for 4 weeks. The results showed that the explants cultured in BA 2 mg/L had the highest shoot induction rate (17.91, 19.1 and 18.67 shoots/explant, respectively). Three parts of the plant were cultured in BA medium at 1.0 mg/L with NAA at concentrations in a range of 0.5-2.0 mg/L. It was found that cotyledons were cultured on 1.0 mg/L BA and 1.5 mg/L NAA. Leaf pieces were cultured on 1.0 mg/L BA with NAA 0.5 mg/L. And the segments were cultured on 1.0 mg/L BA with NAA 0.5 mg/L. The average numbers of shoots were 18.08, 16.91 and 10.50 per shoot, respectively.&nbsp;</p><p><strong>Keywords:</strong> leaf; cotyledon; internode; tissue culture</p> ER -