TY - JOUR AU - สุวรัตน์, สิริมา AU - พงษ์เจริญกิต, แสงทอง AU - คบพิมาย, ยุพเยาว์ AU - วิริยะอลงกรณ์, วินัย AU - สะระ, จันทร์เพ็ญ AU - จีนะเจริญ, ศรัณย์ PY - 2019/04/29 Y2 - 2024/03/29 TI - ความสัมพันธ์ทางพันธุกรรมของลิ้นจี่จากเครื่องหมายโมเลกุลไอเอสเอสอาร์ ลำดับนิวคลีโอไทด์ของยีน rbcL และบริเวณระหว่างยีน trnL-trnF JF - Thai Journal of Science and Technology JA - Thai J. Sci. Technol. VL - 8 IS - 3 SE - วิทยาศาสตร์ชีวภาพ DO - 10.14456/tjst.2019.36 UR - https://li01.tci-thaijo.org/index.php/tjst/article/view/186052 SP - 271-286 AB - <p><strong>Abstract</strong></p><p>Lychee (<em>Litchi chinensis</em> Sonn.) is an economically important fruit crop in Thailand, mainly cultivating in the northern and central part of the country. The popular cultivars are Hong Huai, Ohia, Kimjeng, Chakraphat and Khom. Currently, climate changes cause on-off flowering of lychee, and therefore many farmers have stopped growing the crop which may lead to a loss of traditional cultivars. Meajo University thus have collected and conserved many lychee cultivars in the germplasm field. In establishing a genetic database, it is necessary to classify lychee cultivars using molecular markers. This research aimed to genetically identify 23 lychee cultivars using 20 ISSR (inter-simple sequence repeat) markers and DNA sequences of <em>rbcL</em> gene together with <em>trnL-trnF</em> intergenic spacer region. The results showed that ISSR markers produced 91 bands, 79 (86.81 %) of which were polymorphic. The genetic relationship analysis revealed the similarity coefficients among lychee cultivars ranged from 0.529-1.000, and the lychee samples were categorized into 3 groups: group I consisted of cultivars grown in the central part of the country; group II consisted of cultivars from central and northern part and group III composed of those from the northern part. However, three cultivar pairs were not genetically separated. Analysis of DNA sequences of <em>rbcL</em> gene and <em>trnL-trnF</em> intergenic spacer region showed the genetic distance coefficients varied from 0.0000-0.0035, where Jeen Daeng cultivar was different from other cultivars. When analyzing genetic relationships using longan as an outgroup, lychee cultivars were separated from longan but among lychee cultivars could not be distinguished, except Jeen Daeng cultivar.&nbsp;</p><p><strong>Keywords:</strong> <em>Litchi chinensis</em>; ISSR marker; <em>trnL-trnF</em> gene;<em> rbcL</em> gene</p> ER -