TY - JOUR AU - ณ ราชสีมา, ลลิตา AU - คำโมนะ, กนกภรณ์ AU - พงษ์เจริญกิต, แสงทอง AU - แสงทอง, วราภรณ์ AU - เข็มกลัดเงิน, นฤมล AU - สกูลสิงหาโรจน์, ช่อทิพา AU - ชาวโพงพาง, ศรีเมฆ PY - 2019/06/23 Y2 - 2024/03/29 TI - การถ่ายยีนและวิเคราะห์การแสดงออกของยีน OsB2 ที่ควบคุมการสังเคราะห์แอนโทไซยานินในข้าว JF - Thai Journal of Science and Technology JA - Thai J. Sci. Technol. VL - 8 IS - 5 SE - วิทยาศาสตร์ชีวภาพ DO - 10.14456/tjst.2019.57 UR - https://li01.tci-thaijo.org/index.php/tjst/article/view/197025 SP - 517-531 AB - <p><strong>Abstract</strong></p><p>Anthocyanins are important antioxidants found in black pericarp rice. The <em>OsB</em><em>2</em> gene encodes a transcription factor which can activate anthocyanin biosynthesis in rice. In this study, transformation of Kasalath variety (red pericarp rice) using <em>OsB</em><em>2</em> cloned from a black pericarp rice <em>via</em> <em>Agrobacterium</em> was investigated for functional study on anthocyanin biosynthesis in rice. Fifty-nine transformants were regenerated and PCR analysis showed that 84.28 % carried the <em>OsB2 </em>transgene. The phenotypic traits of transgenic rice were similar to those of wild-type but developing and mature seeds showed purple pigmentation on their pericarp. Segregation analysis of<em> gus</em> genes showed the 3 : 1 ratio in T<sub>1</sub> generation, indicating a single gene insertion in the rice genome. Phenotypic traits of T<sub>1</sub> progeny were similar to those of the T<sub>0</sub> transgenic rice plants. Semi-quantitative RT-PCR analysis revealed that overexpression of <em>OsB</em><em>2</em> gene in seedlings was found in T<sub>1</sub> plants. In addition, seeds of T<sub>1</sub> transgenic rice contained higher antioxidant activity than that of the untransformed plants.&nbsp;</p><p><strong>Keywords:</strong> <em>Oryza sativa</em>; <em>OsB</em><em>2</em>; transformation; anthocyanin</p> ER -