Efficacy of Adjuvanted Streptococcus agalactiae Vaccine by Montanide ISA 763 A VG in Nile tilapia (Oreochromis niloticus Linn.)
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Published:
Dec 31, 2018
Keywords:
Adjuvant Antibody Booster Nile tilapia Streptococcus agalactiae Vaccine
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Abstract
This research investigated the efficacy of adjuvant (Montanide ISA 763 A VG) at concentrations of 0, 5, 10, 20 and 50% in formalin-killed Streptococcus agalactiae vaccine in Nile tilapia (Oreochromis niloticus Linn.). The results showed that adjuvant at 20 and 50% induced significantly higher protection (survival and RPS value) than other groups after three months of experimental trial (P < 0.05). However, only the antibody titer of 20% adjuvant was significantly higher than the control group (P < 0.05). The second experiment aimed to find the efficacy of a single vaccination with 10 and 20% adjuvant compared with non-adjuvant vaccine with a booster dose. The results showed that after the booster vaccination without adjuvant, the antibody titer was significantly higher than other treatments (P < 0.05). However, in terms of protection (survival and RPS), the vaccines with both 10 and 20% adjuvant showed similar efficacy to the booster vaccination until the third month of the trial (P > 0.05), and were significantly different from the vaccine without adjuvant (non-booster group) and from the control (non-vaccination) (P < 0.05). Thus, adjuvant vaccine was as effective as booster vaccination for the first three months of the four-month trial. Further study with the aim to extend protection with a single dose of adjuvant vaccine is being conducted.
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Tepparin, S., Unajak, S., Hirono, I., Kondo, H., & Areechon, N. (2018). Efficacy of Adjuvanted Streptococcus agalactiae Vaccine by Montanide ISA 763 A VG in Nile tilapia (Oreochromis niloticus Linn.). Journal of Fisheries and Environment, 42(3), 26–38. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/136638
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References
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16. Kannika, K., D. Pisuttharachai, P. Srisapoome, J. Wongtavatchai, H. Kondo, I. Hirono, S. Unajak and N. Areechon. 2017. Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR. Journal of Applied Microbiology 122: 1497-1507.
17. Kayansamruaj, P., N. Pirarat, T. Katagiri, I. Hirono and C. Roodkhum. 2014. Molecular characterization and virulence gene profiling of pathogenic Streptococcus agalactiae population from tilapia (Oreochromis sp.) farms in Thailand. Journal of Veterinary Diagnostic Investigation 26(4): 488-495.
18. Klesius, P. H., C. A. Shoemaker and J. J. Evans. 2000. Efficacy of single and combined Streptococcus iniae isolate vaccine administered by intraperitoneal and intramuscular routes in tilapia (Oreochromis niloticus). Aquaculture 188: 237-246.
19. Li, L. P., R. Wang, W. W. Liang, T. Huang, Y. Huang, F. G. Luo, A. Y. Lei, M. Chen and X. Gan. 2015. Development of live attenuated Streptococcus agalactiae vaccine for tilapia via continuous passage in vitro. Fish & Shellfish Immunology 45: 955-963.
20. Lydyard, P., A. Whelan and M. Fanger. 2011. Immunology. 3rd edition. Garland Science, Taylor & Francis Group, New York and London. 358 pp.
21. Mutwiri, G., V. Gerdts, M. Lopez and L. A. Babiuk. 2007. Innate immunity and new adjuvants. Revue scientifique et technique (International Office of Epizootics) 26(1): 147-156.
22. Noia, M., B. Domínguez, J. Leiro, L. Blano-Méndez, A. Luzardo-Álvares and J. Lamas. 2014. Inflamatory responses and side effects generated by several adjuvant-containing vaccines in turbot. Fish & Shellfish Immunology 38: 244-254.
23. Ravelo, C., B. Magariños, M. C. Herrero, L. Costa, A. E. Toranzo and J. L. Romalde. 2006. Use of adjuvanted vaccines to lengthen the protection against lactococcosis in rainbow trout (Oncorhynchus mykiss). Aquaculture 251: 153-158.
24. Roldan, M. A. M. 2014. Development of a vaccine against Francisella noatunensis subsp. orientalis in red Nile tilapia (Oreochromis niloticus). Thesis, Institute of Aquaculture, University of Stirling. 38 pp.
25. Shoemaker, C. A., B. R. LaFrentz and P. H. Klesius. 2011. Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus x O. aureus) with an inactivated Vibrio vulnificus vaccine. Biologicals 39(6): 424-429.
26. Suanyuk, N., F. Kong, D. Ko, G. L. Gilbert and K. Supamattaya. 2008. Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. Nile tilapia O. niloticus in Thailand-Relationship to human isolates?. Aquaculture 284: 35-40.
27. Tafalla, C., J. Bøgwald and R. A. Dalmo. 2013. Adjuvant and immunostimulants in fish vaccine: Current knowledge and future perspectives. Fish & Shellfish Immunology 35: 1740-1750.
28. Wang, Y.T., H. Y. Huang, M. A. Tsai, P. C. Wang and B. H. Jiang. 2014. Phosphoglycerate kinase enhanced immunity of the whole cell of Streptococcus agalactiae in tilapia, Oreochromis niloticus. Fish & Shellfish Immunology 41: 250-259.
2. Aminudin, S. A., F. M. Kamal, M. Zamri-Saad, S.Z. Abdullah, M. S. Ridzuan, H. M. Yusoff, S. Hashim, F. Sudirwan, I. Md. Salihin and S.A. Sulaiman. 2018. Effect of incorporating different concentrations of palm oil as adjuvant in fish vaccine. International Journal of Biosciences 12(1): 35-41.
3. Awate, S., L. A. Babiuk and G. Mutwiri. 2013. Mechanisms of action of adjuvants. Frontiers in Immunology 4: 114.
4. Bastardo, A., C. Ravelo, N. Castro, J. Calheiros and J. L. Romalde. 2012. Effectiveness of bivalent vaccine against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynhus mykiss (Walbaum). Fish & Shellfish Immunology 32: 756-761.
5. Bowden, T. J., K. Adamson, P. MacLachlan, C. C. Pert and I. R. Bricknell. 2003. Long-term study of antibody response and injection-site effect of oil adjuvants in Atlantic halibut (Hippoglossus hippoglossus L.). Fish & Shellfish Immunology 14: 363-369.
6. Bøgwald, J. and R. A. Dalmo. 2012. Developments in Adjuvants for Fish Vaccines. In: Infectious diseases in aquaculture prevention and control (ed. B. Austin), pp. 244-274. Woodhead Publishing Limited, UK.
7. Cao, T. T., M. A. Tsai, C. D. Yang, P. C. Wang, T. Y. Kuo, H. C. G. Chen and S. C. Chen. 2014. Vaccine efficacy of glyceraldehyde - 3 - phosphate dehydrogenase (GAPDH) from Edwardsiella ictaluri against E. tarda in tilapia. Journal of General and Applied Microbiology 60: 241-250.
8. Dangwetngam, M., N. Suanyuk, F. Kong and W. Phromkunthong. 2016. Serotype distribution and antimicrobial susceptibilities of Streptococcus agalactiae isolated from infected cultured tilapia (Oreochromis niloticus) in Thailand: Nine-year perspective. Journal of Medical Microbiology 65: 247–254.
9. Delannoy, C. M. J., M. Crumlish, M. C. Fontaine, J. Pollock, G. Foster, M. P. Dagleish, J. F. Turnbull and R. N. Zadoks. 2013. Human Streptococcus agalactiae strain in aquatic mammals and fish. BMC Microbiology 13: 41.
10. Evans, J. J., D. J. Pasnik, P. H. Klesius and C. A. Shoemaker. 2006. Identification and epidemiology of Streptococcus iniae and Streptococcus agalactiae in tilapia, Oreochromis spp. Proceedings of the 7th International Symposium on Tilapia in Aquaculture 2006: 25-42.
11. He. Y., K. Y. Wang, D. Xiao, D. F. Chen, L. Huang, T. Liu, J. Wang, Y. Geng, E. I. Wang and Q. Yang. 2014. A recombinant truncated surface immunogenic protein (tsip) plus Adjuvant FIA confers active protection against Group B Streptococcus infection in tilapia. Vaccine 32: 7025-7032.
12. Huang, H. Y., Y. C. Chen, P. C. Wang, M. A. Tsai, S. C. Yeh, H. J. Liang and S. C. Chen. 2014. Efficacy of formalin - inactivated vaccine against Streptococcus iniae infection in the farmed grouper Epinephelus coioides by intraperitoneal immunization. Vaccine 32: 7014-7020.
13. Imperi, M., M. Pataracchia, G. Alfarone, L. Baldassarri, G. Orefici and R. Creti. 2010. A multiplex PCR assay for the direct indentification of the capsular type (Ia to IX) of Streptococcus agalactiae. Journal of Microbiological Methods 80(2): 212-214.
14. Ismail, M. S., A. Siti-Zahrah, M. R. M. Syafiq, M. N. A. Amal, M. Firdaus-Nawi and M. Zamri-Saad. 2016. Feed-based vaccination regime against Streptococcosis in red tilapia, Oreochromis niloticus x Oreochromis mossambicus. BMC Veterinary Research 12: 194.
15. Jaafar, R. M., J. K. Chettri, I. Dalsgaard, A. Al-jubury, P. W. Kania, J. Skov and K. Buchmann. 2015. Effects of adjuvant MontanideTM ISA 763 A VG in rainbow trout injection vaccinated against Yersinia ruckeri. Fish & Shellfish Immunology 47: 797-806.
16. Kannika, K., D. Pisuttharachai, P. Srisapoome, J. Wongtavatchai, H. Kondo, I. Hirono, S. Unajak and N. Areechon. 2017. Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR. Journal of Applied Microbiology 122: 1497-1507.
17. Kayansamruaj, P., N. Pirarat, T. Katagiri, I. Hirono and C. Roodkhum. 2014. Molecular characterization and virulence gene profiling of pathogenic Streptococcus agalactiae population from tilapia (Oreochromis sp.) farms in Thailand. Journal of Veterinary Diagnostic Investigation 26(4): 488-495.
18. Klesius, P. H., C. A. Shoemaker and J. J. Evans. 2000. Efficacy of single and combined Streptococcus iniae isolate vaccine administered by intraperitoneal and intramuscular routes in tilapia (Oreochromis niloticus). Aquaculture 188: 237-246.
19. Li, L. P., R. Wang, W. W. Liang, T. Huang, Y. Huang, F. G. Luo, A. Y. Lei, M. Chen and X. Gan. 2015. Development of live attenuated Streptococcus agalactiae vaccine for tilapia via continuous passage in vitro. Fish & Shellfish Immunology 45: 955-963.
20. Lydyard, P., A. Whelan and M. Fanger. 2011. Immunology. 3rd edition. Garland Science, Taylor & Francis Group, New York and London. 358 pp.
21. Mutwiri, G., V. Gerdts, M. Lopez and L. A. Babiuk. 2007. Innate immunity and new adjuvants. Revue scientifique et technique (International Office of Epizootics) 26(1): 147-156.
22. Noia, M., B. Domínguez, J. Leiro, L. Blano-Méndez, A. Luzardo-Álvares and J. Lamas. 2014. Inflamatory responses and side effects generated by several adjuvant-containing vaccines in turbot. Fish & Shellfish Immunology 38: 244-254.
23. Ravelo, C., B. Magariños, M. C. Herrero, L. Costa, A. E. Toranzo and J. L. Romalde. 2006. Use of adjuvanted vaccines to lengthen the protection against lactococcosis in rainbow trout (Oncorhynchus mykiss). Aquaculture 251: 153-158.
24. Roldan, M. A. M. 2014. Development of a vaccine against Francisella noatunensis subsp. orientalis in red Nile tilapia (Oreochromis niloticus). Thesis, Institute of Aquaculture, University of Stirling. 38 pp.
25. Shoemaker, C. A., B. R. LaFrentz and P. H. Klesius. 2011. Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus x O. aureus) with an inactivated Vibrio vulnificus vaccine. Biologicals 39(6): 424-429.
26. Suanyuk, N., F. Kong, D. Ko, G. L. Gilbert and K. Supamattaya. 2008. Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. Nile tilapia O. niloticus in Thailand-Relationship to human isolates?. Aquaculture 284: 35-40.
27. Tafalla, C., J. Bøgwald and R. A. Dalmo. 2013. Adjuvant and immunostimulants in fish vaccine: Current knowledge and future perspectives. Fish & Shellfish Immunology 35: 1740-1750.
28. Wang, Y.T., H. Y. Huang, M. A. Tsai, P. C. Wang and B. H. Jiang. 2014. Phosphoglycerate kinase enhanced immunity of the whole cell of Streptococcus agalactiae in tilapia, Oreochromis niloticus. Fish & Shellfish Immunology 41: 250-259.