Morphological and Histological Study on the Development of the Digestive System of Siamese Spiny Eel, Macrognathus siamensis (Günther, 1861) Larvae

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Theerachi Pongjanyakul
Nitikorn Piwpong
Kanjana Payooha
Praneet Ngamsnae
Thanatip Leamkom
Jarungjit Grudpan

Abstract

Natural populations of the Siamese spiny eel, Macrognathus siamensis (Günther, 1861) have drastically declined while their selling price has been rapidly increasing. For the culture of this species, knowledge of the exact duration of the stages of morphological development of the digestive system would help keepers match appropriate types of food with the changing needs of the larvae. Brood-stocks (10 pairs) of Siamese spiny eel were obtained from the Inland Fisheries Research and Development Regional Center 4 in Ubon Ratchathani Province and artificially induced to spawn by injecting buserelin acetate at 30 µg·kg-1 and domperidone at 10 mg·kg-1. After hatching, 20 fish larvae were serially sampled every two days, from 3 dph (day post hatching) to 40 dph. Fish specimens were fixed in 10% formaldehyde solution and processed for microtome study. Results showed that after hatching, the alimentary canal of the Siamese spiny eel was a straight tube, attached dorsally to the yolk sac. Differentiation of alimentary canal into buccopharynx, esophagus, and anterior and posterior intestine was found at 3 dph, along with the development of other accessory organs, such as liver, pancreas and gall bladder. During 7-9 dph, stomach divided into the cardiac, fundic and pyloric parts. At 11 dph, full development of gastric glands was found along the fundic region, as well as the appearance of the pyloric sphincter, which enabled us to discern the stomach from anterior intestine. From 13 dph until the end of the experiment (40 dph), there was no notable differentiation of the digestive tract except for increasing size. The results suggest that weaning of larvae would be appropriate at 13 dph, according to its fully developed digestive system.

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1. Cahu, C.L. and J.L. Zambonino-Infante. 2001. Substitution of live food by formulated diets in marine fish larvae. Aquaculture 200(1–2): 161–180.

2. Cañavate, J.P. and C. Fernández-Dı́az. 1999. Influence of co-feeding larvae with live and inert diets on weaning the sole Solea senegalensis onto commercial dry feeds. Aquaculture 174(3–4): 255–263.

3. Chen, B.N., J.G. Qin, M.S. Kumar, W.G. Hutchinson and S.M. Clarke. 2006. Ontogenetic development of digestive enzymes in yellowtail kingfish Seriola lalandi larvae. Aquaculture 260(1–4): 264–271.

4. Cousin, J.C.B. and F.B. Laurencin. 1985. Morphogenesis of the digestive system and swim bladder of the turbot, Scophthalmus maximus L. Aquaculture 47(4): 305–319.

5. Cyrino, J.E.P., D.P. Bureau and B.G. Kapool. 2008. Feeding and digestive functions in fishes. CRC Press, Florida. 597 pp.

6. Dai, X., M. Shu and W. Fang. 2007. Histological and ultrastructure study of the digestive tract of rice field eel, Monopterus albus. Journal of Applied Ichthyology 23(2): 177–183.

7. Faulk, C.K., A.D. Benninghoff and G.J. Holt. 2007. Ontogeny of the gastrointestinal tract and selected digestive enzymes in cobia Rachycentron canadum (L.). Journal of Fish Biology 70(2): 567–583.

8. Frías-Quintana, C.A., G. Márquez-Couturier, C.A. Alvarez-González, D. Tovar-Ramírez, H. Nolasco-Soria, M.A. Galaviz-Espinosa and E. Gisbert. 2015. Development of digestive tract and enzyme activities during the early ontogeny of the tropical gar Atractosteus tropicus. Fish Physiology and Biochemistry 41(5): 1075–1091.

9. Gentea, F., E. Terwinghe and A. Danguy. 2009. Atlas of fish histology. Science Publishers, New Hampshire, USA. 215 pp.

10. Guyot, E., J.P. Diaz and R. Connes. 1995. Organogenesis of the liver in sea bream. Journal of Fish Biology 47(3): 427–437.

11. Guyot, E., J.P. Diaz, B. Romestand and R. Connes. 1998. Insulin during the early postembryonic development of gilt-head sea bream, Sparus aurata: ultrastructural, immunohistochemical, and biochemical studies. General and Comparative Endocrinology 110: 146–156.

12. Gilbert, E., R.H. Piedrahita and D.E. Conklin. 2004. Ontogenetic development of the digestive system in California halibut (Paralichthys californicus) with notes on feeding practices. Aquaculture 232(1): 455–470.

13. Gilbert, E., M.C. Sarasquete, P. Williot and F. Castelló-Orvay. 1999. Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny. Journal of Fish Biology 55(3): 596–616.

14. Gisbert, E., C. Moreira, D. Castro-Ruiz, S. Öztürk, C.F. Mendez, S. Gilles, J. Núñez-Rodriguez, F. Duponchelle, S. Tello, J.F. Renno, C. Davila and M. Darias. 2014. Histological development of the digestive system of the Amazonian pimelodid catfish Pseudoplatystoma punctifer. Animal 8(11): 1765–1776.

15. Hachero-Cruzado, I., J.B. Ortiz-Delgado, B. Borrega, M. Herrera, J.I. Navas and C. Sarasquete. 2009. Larval organogenesis of flatfish brill Scophthalmus rhombus L: Histological and histochemical aspects. Aquaculture 286(1): 138–149.

16. Hoehne-Reitan, K., E. Kjørsvik and D.R. Gjellesvik. 2001. Development of bile salt-dependent lipase in larval turbot. Journal of Fish Biology 58(3): 737–745.

17. Humason, G.L. 1979. Animal tissue technique. W.H. Freeman and Company. San-Francisco. 661 pp.

18. Jaroszewska, M. and K. Dabrowski. 2011. Utilization of yolk: transition from endogenous to exogenous nutrition in fish. In: Larval fish nutrition (ed. G.J. Holt), pp. 183–218. Wiley-Blackwell, Oxford.

19. Kapoor, B.G., H. Smith and I.A. Verighina. 1975. The alimentary canal and digestion in teleosts. Advances in Marine Biology 13: 109–239.

20. Khachonpisitsak, S. 2007. Taxonomy of spiny eels (Symbranchiformes: Mastacembelidae) in Thailand. Master’s Thesis, Chulalongkorn University, Bangkok, Thailand. 260 pp.

21. Knutsen, H.R. 2015. Morphological development of wild leptocephalus larvae of the European eel (Anguilla anguilla)-with special emphasis on muscle and digestive system. https://brage.bibsys.no/xmlui/handle/11250/2358867. Cited 3 Jan 2018.

22. Machado, M., H. Souza, V. Lemos de Souza, A. De Azevedo, R. Goitein and A.D. Nobre. 2013. Morphological and anatomical characterization of the digestive tract of Centropomus parallelus and C. undecimalis. Acta Scientiarum-Biological Sciences 35: 467–474.

23. Micale, V., M. Garaffo, L. Genovese, M.T. Spedicato and U. Muglia. 2006. The ontogeny of the alimentary tract during larval development in common pandora Pagellus erythrinus L. Aquaculture 251(2–4): 354–365.

24. Mitra, A., K.M. Pratap and H. Sumit. 2015. Histomorphological study of the gut developmental pattern in early life histology stages of featherback, Chitala chitala (Hamilton). Archives of Polish Fisheries 23: 25–35.

25. Ostaszewska, T., K. Krajnik, D. Adamek-Urbańska, R. Kasprzak, M. Rzepkowska, M. Luczynski and K. Dabrowski. 2018. Effect of feeding strategy on digestive tract morphology and physiology of lake whitefish (Coregonus lavaretus). Aquaculture 497: 32–41.

26. Paray, B.A., M.A. Haniffa, M.U.D. War, M.K. Al-Sadoon, Y.H. Park and I.A. Rather. 2015. Histological changes in the digestive tract of striped murrel larvae during ontogeny. Indian Journal of Geo-Marine Sciences 44(7): 984–992.

27. Peña, R., S. Dumas and M. Contreras-Olguín. 2017. Organogenesis of the digestive system in Pacific red snapper (Lutjanus peru) larvae. Aquaculture Research 48(4): 1561–1575.

28. Pradhan, P., K.J. Jena, G. Mitra, N. Sood and E. Gisbert. 2012. Ontogeny of the digestive tract in butter catfish Ompok bimaculatus (Bloch) larvae. Fish Physiology and Biochemistry 38: 1601–1617.

29. Rainboth, W.J. 1996. Fishes of the Cambodia Mekong. Food and Agriculture Organization of The United States. Rome. 265 pp.

30. Rangsin, W., N. Areechon and R. Yoonpundh. 2012. Digestive enzymes activities during larval development of striped catfish, Pangasianodon hypopthalmus (Sauvage, 1878). Kasetsart Journal: Natural Science 46: 217–228.

31. Rønnestad, I., Y. Manuel, U. Bernd, R. Laura, S. øystein and B. Clara. 2013. Feeding behavior and digestive physiology in larval fish: current knowledge, and gaps and bottlenecks in research. Reviews in Aquaculture 5 (Suppl. 1): 59–98.

32. Ribeiro, L., C. Sarasquete and M.T. Dinis. 1999. Histological and histochemical development of the digestive system of Solea senegalensis (Kaup, 1858) larvae. Aquaculture 171(3–4): 293–308.

33. Sánchez-Amaya, M.I., J.B. Ortiz-Delgado, Á. García-López, S. Cárdenas and C. Sarasquete. 2007. Larval ontogeny of redbanded seabream Pagrus auriga Valenciennes, 1843 with special reference to the digestive system. A histological and histochemical approach. Aquaculture 263(1–4): 259–279.

34. Santhanam, R. 2015. Nutritional freshwater life. CRC Press. Florida. 215 pp.

35. Saowakoon, H. and S. Saowakoon. 2007. Breeding and nursing of spotted spiny eel (Macrognathus siamensis; Gunther, 1861). Proceedings of the 45th Kasetsart University Annual Conference: Fisheries Section 2007: 712–721.

36. Sarasquete, C., J.M. González de Canales, J.A. Arellano, A. Muñoz-Cueto, L. Ribeiro and M.T. Dinis. 1996. Histochemical aspects of the yolk-sac and digestive tract of larvae of the Senegal sole, Solea senegalensis. Histology and Histopathology 11: 881–888.

37. Smith, S.L. 1989. Digestive function in teleost fishes. In: Fish nutrition (ed. J.E. Halver), pp. 331–421. Academic Press. San Diego.

38. Stroband, H.W.J. and A.G. Kroon. 1981. The development of the stomach in Clarias lazera and the intestinal absorption of protein macromolecules. Cell and Tissue Research 215(2): 397–415.

39. Thalathiah, S.B., A.B.O. Ahmad and B.S. Mohamad Zaini. 1988. Induced spawning of Asian fishes induced spawning techniques practised at Batu Berendam, Melaka, Malaysia. Aquaculture 74(1): 23–33.

40. Thilsted, S.H. 2010. The potential of nutrition-rich small fish species in aquaculture to improve human nutrition and health. Proceedings of the Global Conference on Aquaculture 2010: 57–73.

41. Verreth, J.A.J., E. Torreele, E. Spazier, A. Van der Sluiszen, J.H.W.M. Rombout, R. Booms and H. Segner. 1992. The development of a functional digestive system in the African catfish Clarias gariepinus (Burchell). Journal of the World Aquaculture Society 23(4): 286–298.

42. Walford, J. and T.J. Lam. 1993. Development of digestive tract and proteolytic enzyme activity in seabass (Lates calcarifer) larvae and juveniles. Aquaculture 109(2): 187–205.

43. Zaiss, M.M., I.E. Papadakis, E. Maingot, P. Divanach and C.C. Mylonas. 2006. Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system. Aquaculture 260(1): 357–368.

44. Zambonino-Infante, J.L., E. Gisbert, C. Sarasquete, I. Navarro, J. Gutiérrez and C.L. Cahu. 2008. Ontogeny and physiology of the digestive system of marine fish larvae. In: Feeding and digestive function of fishes (ed. J.E.O. Cyrino), pp. 281–348. Science Publishers, USA.