Effects of Inorganic Media Enriched with Sodium Acetate on the Growth Performance and Nutrient Content in The Microalga Chlorella vulgaris
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Abstract
Inorganic nutrients are used in aquaculture to increase natural productivity. This study aimed to apply sodium acetate as a growth enhancer in inorganic media to culture the microalga Chlorella vulgaris in laboratory conditions. Sodium acetate-enriched Bold basal medium (BBMAC) and Urea-Phosphate-Potassium media (NPKAC1 and NPKAC2) were used as experimental treatments, while Bold basal medium (BBM) was used as a control. During eight days of culture of C. vulgaris, maximum cell growth of 262.35 ×105 cells·mL-1 and chlorophyll a concentration of 12.16 mg·L-1 were found in BBMAC, followed by those grown in BBM, NPKAC1 and NPKAC2. Specific growth rates (SGR, µ) and chlorophyll a of C. vulgaris grown in BBMAC were significantly (p<0.01) higher than microalgae cultured in other media. Crude protein of C. vulgaris cultured in BBM as control medium was significantly (p<0.01) higher than that of algae grown in other media. Crude lipid content of C. vulgaris grown in BBMAC was significantly (p<0.01) higher than that of algae cultured in other media. However, there was little difference in content of crude lipids among algae grown in BBM and other inorganic media containing sodium acetate. The study indicates that media enriched with sodium acetate can enhance the cell growth and nutrient values in cultivation of C. vulgaris.
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