A Study on the Synergistic Effect of Bioactive Compounds from Papaya Leaf and Collagen Isolated from Indian Mackerel on in vitro Wound Closure Capacity

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Published: Jul 1, 2020
Keywords:
Acid-soluble collagen Indian mackerel Papaya leaf Wound closure

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Nagappa Bailore Niveditha
Biochemistry Division, Department of Chemistry, Mangalore University, Karnataka, India
Balladka Kunhanna Sarojini
Biochemistry Division, Department of Chemistry, Mangalore University, Karnataka, India
Akhila Hosur Shrungeswara
Department of Industrial Chemistry, Mangalore University, Karnataka, India

Abstract

Collagen is an extracellular matrix protein which plays a vital role in the wound healing process. Wound healing properties of papaya leaf extract have been well studied. Therefore, the synergistic effect of papaya leaf phytoconstituents and isolated collagen was evaluated for its wound healing properties. In the present study, acid-soluble collagen (ASC) was isolated from the skin of Indian mackerel (Restrellinger kanagurta) and characterized. Along with isolated ASC, major papaya leaf constituents including caffeic acid, coumaric acid, quercetin and oleic acid were added to study their effects on wound healing. The ASC was isolated with a yield of 3.57 %. On the basis of SDS-PAGE analysis, the isolated ASC was identified as type I collagen. In the FTIR spectra, five amide groups were identified as major bands, which represents the triple helical nature of the collagen. By SEM analysis, the collagen fibers were found to be densely packed. Different combinations of collagen gel and papaya leaf compounds were prepared by one-to-one combination as well as mixed combinations. These test compounds were screened by in vitro scratch wound assays using the NIH3T3 cell line. Among all the test compounds, collagen with quercetin (0.04 mg) was the most effective in enhancing wound closing, with 100 % migration of fibroblasts.

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How to Cite
Niveditha, . N. B., Sarojini, B. K., & Shrungeswara, A. H. (2020). A Study on the Synergistic Effect of Bioactive Compounds from Papaya Leaf and Collagen Isolated from Indian Mackerel on in vitro Wound Closure Capacity. Journal of Fisheries and Environment, 44(2), 24–36. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/202929
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Vol. 44 No. 2 (2020): May-August
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Articles
Author Biography

Nagappa Bailore Niveditha, Biochemistry Division, Department of Chemistry, Mangalore University, Karnataka, India

Biochemistry Division, Department of Chemistry, Mangalore University, Mangalagangothri- 574199, Mangalore, Karnataka, India.

References

Ali, A.M.M., S. Benjakul and H. Kishimura. 2017. Molecular characteristics of acid and pepsin soluble collagens from the scales of golden carp (Probarbus jullieni). Emirates Journal of Food and Agriculture 29: 450–457.

Aruljothi, S., C. Uma, P. Sivagurunathan and M. Bhuvaneswari. 2014. Investigation on antibacterial activity of Carica papaya leaf extracts against wound infection-causing bacteria. International Journal of Research Studies in Bioscience 2: 8–12.

Brett, D. 2008. A review of collagen and collagen-based wound dressings. Wounds 20: 347– 356.

Canini, A., D. Alesiani, G. D’Arcangelo and P. Tagliatesta. 2007. Gas chromatography–mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf. Journal of Food Composition and Analysis 20: 584–590.

Chen, J., L. Li, R. Yi, N. Xu, R. Gao and B. Hong. 2016. Extraction and characterization of acid soluble collagen from scales and skin of tilapia (Oreochromis niloticus). LWT-Food Science and Technology 66: 453–459.

Duan, R., J. Zhang, X. Dua, X. Yao and K. Konno. 2009. Properties of collagen from skin, scale and bone of carp (Cyprinus carpio). Food Chemistry 112: 702–706.

Foegeding, E.A., T.C. Lanier and H.O. Hultin. 1996. Collagen. Food Chemistry 3: 902–906.

Hammed, A.M., J. Irwandi, M.S. Hamzah and A.A. Hassan. 2014. Effects of pretreatment on properties of gelatin from perch (Lates niloticus) skin. International Journal of Food Properties 17: 1224–1236.

Hema, G.S., K. Shyni, S. Mathew, R. Anandan, G. Ninan and P.T. Lakshmanan. 2013. A simple method for isolation of fish skin collagen- biochemical characterization of skin collagen extracted from albacore tuna (Thunnus alalunga), dog shark (Scoliodon sorrakowah) and rohu (Labeo rohita). Annals of Biological Research 4: 271–278.

Jackson, M., P. Chool, P.H. Watson, W.C. Halliday and H.H. Mantsch. 1995. Beware of connective tissue proteins: Assignment and implications of collagen absorption in infrared spectra of human tissues. Biochimica et Biophysica Acta 1270: 1–6.

Jongjareonrak, A., S. Benjakul, W. Visessanguan, T. Nagai and M. Tanaka. 2005a. Isolation and characterization of acid and pepsin-solubilised collagens from the skin of brown stripe red snapper (Lutjanus vitta). Food Chemistry 93: 475–484.

Jongjareonrak, A., S. Benjakul, W. Visessanguan and M. Tanaka. 2005b. Isolation and characterization of collagen from bigeye snapper (Priacanthus macracanthus) skin. Journal of the Science of Food and Agriculture 85: 1203–1210.

Khan, S.B., Z.J. Qian, B. Ryu and S.K. Kim. 2009. Isolation and biochemical characterization of collagens from seaweed pipefish (Syngnathus schlegeli). Bio Technology and Bio Process Engineering 14: 436–442.

Kirti and S.S. Khora. 2015. Isolation and characterization of collagens extracted from the skin of pufferfish (Lagocephalus wheeleri). Research Journal of Pharmaceutical Biological and Chemical Sciences 6: 863–872.

Kittiphattanabawon, P., S. Benjakul, W. Visessanguan, T. Nagai and M. Tanaka. 2005. Characterization of acid-soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chemistry 89: 363–372.

Kittiphattanabawon, P., S. Benjakul, W. Visessanguan and F. Shahidi. 2010. Isolation and properties of acid and pepsin-soluble collagen from the skin of blacktip shark (Carcharhinus limbatus). European Food Research and Technology 230: 475–483.

Krishnappa, P., K. Venkatarangaiah, S.K.S. Rajanna and R.K. Balan. 2016. Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats. Journal of Pharmaceutical Analysis 6: 389–395.

Laemmli, U.K. 1970. Cleavage of structural proteins during assembly head of bacteriophage T4. Nature 277: 680–685.

Lewis, M.S. and K.A. Piez. 1964. The characterization of collagen from the skin of the dogfish shark (Squalus acanthias). Journal of Biological Chemistry 239: 3336–3340.

Lin, Y.K., T.Y. Lin and H.P. Su. 2001. Extraction and characterization of telopeptide-poor collagen from porcine lung. Food Chemistry 124: 1583–1588.

Lin, Y.K. and D.C. Liu. 2006. Effects of pepsin digestion at different temperatures and times on properties of telopeptide-poor collagen from bird feet. Food Chemistry 94: 621–625.

Liu, W., G. Li, Y. Miao and X. Wu. 2009. Preparation and characterization of pepsin solubilized type I collagen from the fish scale snakehead (Ophiocephalus argus). Journal of Food Biochemistry 33: 20–37.

Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265–275.

Monsur, H.A., I. Jaswir, H.M. Salleh and H.A. Alkahtani. 2014. Effects of pretreatment on properties of gelatin from perch (Lates niloticus) skin. International Journal of Food Properties 17: 1224–1236.

Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55–63.

Muniandy, K., S. Gothai, W.S. Tan, S.S. Kumar, N.M. Esa, G. Chandramohan and P. Arulselvan. 2018. In vitro wound healing potential of stem extract of Alternanthera sessilis. Evidence Based Complementary and Alternative Medicine 2018: DOI 10.1155/2018/3142073

Muralidharan, N., R.J. Shakila, D. Sukumar and G. Jeyasekaran. 2013. Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization. Journal of Food Science and Technology 50: 1106–1113.

Muyonga, J.H., C.G.B. Cole and K.G. Duodu. 2004. Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chemistry 86: 325–332.

Na, G.C. 1986. Interaction of calf skin collagen with glycerol: linked function analysis. Biochemistry 25: 967–973.

Nagai, T., Y. Araki and N. Suzuki. 2002. Collagen of the skin of ocellate puffer fish (Takifugu rubripes). Food Chemistry 78: 173–177.

Nagai, T. and N. Suzuki. 2000. Isolation of collagen from fish waste material-skin, bone, and fins. Food Chemistry 68: 277–281.

Ogawa, M., M.W. Moody, R.J. Portier, J. Bell, M.A. Schexnayder and J.N. Losso. 2003. Biochemical properties of black drum and sheepshead seabream skin collagen. Journal of Agricultural and Food Chemistry 51: 8088–8092.

Parks, W.C. 1995. The production, role and regulation of matrix metallo proteinases in the healing epidermis. Wounds 7: 23–37.

Pati, F., B. Adhikari and S. Dhara. 2010. Isolation and characterization of fish scale collagen of higher thermal stability. Bioresource Technology 101: 3737–3742.

Rajkumar, S.R.J., G. Gnanavel, M.M. Nadar and R. Sankaranarayanan. 2018. Wound healing activity of Morinda tinctoria roxb aqueous leaf extract. Biotech 8: 343. DOI: 10.1007/s13205-018-1361-5.

Rangaraj, A., K.G. Harding and D. Leaper. 2011. Role of collagen in wound management. Wounds 7: 54–63.

Senaratne, L.S., P.J. Park and S.K. Kim. 2006. Isolation and characterization of collagen from brown backed toadfish (Lagocephalus gloveri) skin. Bioresource Technology 97: 191–197.

Shanmugam, V., P. Ramasamy, N. Subhapradha, S. Sudharsan, P. Seedevi, M. Moovendhan and A. Srinivasan. 2012. Extraction, structural and physical characterization of type I collagen from the outer skin of Sepiella inermis (Orbigny, 1848). African Journal of Biotechnology 11: 14326–14337.

Singh, P., S. Benjakul, S. Maqsood and H. Kishimura. 2011. Isolation and characterization of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry 124: 97–105.

Sionkowska, A., J. Kozlowska, M. Skorupska and M. Michalska. 2015. Isolation and characterization of collagen from the skin of Brama australi. International Journal of Biological Macromolecules 80: 605–609.

Sonavane, A.E., J.M. Koli, S.B. Patange, S.D. Naik and A.S. Mohite. 2018. Isolation of acid and pepsin soluble collagens from the skin of Indian mackerel Rastrelliger kanagurta (Cuvier, 1817). Journal of Entomology and Zoology Studies 6: 2508–2511.

Sripriya, R. and R. Kumar. 2015. A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish Rohu (Labeo rohita). Food and Nutrition Sciences 6: 1468 -1478.

Stoscheck, C.M. 1990. Quantitation of protein. In Methods in Enzymology 182: 50-68.

Stout, E.I. and A. Mckessor. 2012. Glycerin-based hydrogel for infection control. Advances in Wound Care 1: 48–51.

Suarez, H., O. Gaitan and C. Díaz. 2015. Microstructural and physicochemical analysis of collagen in intramuscular pin bones of Bocachico fish (Prochilodus sp.). Revista Colombiana de Ciencias Pecuarias 28: 188–196.

Suntar, I.P., E.K. Akkol, F.N. Yalçın, U. Koca, H. Keleş and E. Yesilada. 2010. Wound healing potential of Sambucus ebulus L. leaves and isolation of an active component, quercetin 3-O-glucoside. Journal of Ethnopharmacology 129: 106–114.

Tabarestani, H.S., Y. Maghsoudlou, A. Motamedzadegan, A.R. Mahoonak and H. Rostamzad. 2012. Study on some properties of acid-soluble collagens isolated from fish skin and bones of rainbow trout (Oncorhynchus mykiss). International Food Research Journal 19: 251–257.

Veeruraj, A., M. Arumugam and T. Balasubramanian. 2013. Isolation and characterization of thermostable collagen from the marine eel-fish (Evenchelys macrura). Process Biochemistry 48: 1592–1602.

Vowden, P. 2011. Hard-to-heal wounds Made Easy. Wounds 2: 1-6.

Weber, L., E. Kirsch and P. Muller. 1984. Collagen type distribution and macromolecular organization of connective tissue in different layers of human skin. Journal of Investigative Dermatology 82: 156–60.

Yamaguchi, K., J. Lavety and R.M. Love. 1976. The connective tissues of fish VIII. Comparative studies on hake, cod and catfish collagens. International Journal of Food Science Technology 11: 389–399.