The Effect of Rubber Seed (Havea brasiliensis) as Anesthetic on Asian Sea Bass (Lates calcarifer, Centropomidae) during Transport

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Megananda Paradewi
Dhita Maulida Saputri
Laksmi Sulmartiwi
Hari Suprapto
Kriengkrai Satapornvanit


The Asian sea bass is one of the most important marine aquaculture species in Indonesia. Transportation is often required for the distribution of sea bass fingerling, but this leads to stress on fish, as reflected by cortisol and blood glucose levels. Stress in fish during transportation can be suppressed using anesthetic substances. This study aims to determine the effectiveness of rubber seed extract as an anesthetic for Asian sea bass fingerling during transportation, with cortisol and blood glucose levels, and survival rate as indicators. The treatments consisted of four concentrations of rubber seed extract (70 ppm, 80 ppm, 90 ppm and 100 ppm), 5 ppm clove oil as a positive control, and water without any anesthetic as a negative control. Results show that rubber seed extract as an anesthetic material has a significant effect (p<0.05) in reducing cortisol and blood glucose levels at six hours of transport, with the optimal concentration of 90 mg·L-1


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1. Begg, K. and N.W. Pankhurst. 2004. Endocrine and metabolic responses to stress in a laboratory population of the tropical damselfish Acanthochromis polyacanthus. Journal of Fish Biology 64: 133–145. DOI: 10.1111/j.1095-8649.2004.00290.

2. Eames, S.C., H.P. Louis, E.P. Victoria and D.K. Mary. 2010. Blood sugar measurement in Zebrafish reveal dynamic of glucose homeostasis. Zebrafish 7(2): 205–213. DOI: 10.1089/zeb.2009.0640.

3. Hanggono, B. 2006. Application clove oil as an anesthetic for sea bass (Lates calcarifer Bloch). Jurnal Perikanan Indonesia 8: 9–16.

4. Hanley, C.S., V.L. Clyde, R.S. Wallace, J. Paulmurphy, T.A. Patterson, N.S. Keuler and K.K. Sladky. 2010. Effects of anesthesia and surgery on serial blood gas values and lactate concentrations in yellow perch (Perca flavescens), walleye pike (Sander vitreus), and koi (Cyprinus carpio). Journal of the American Veterinary Medical Association 236: 1104–1108.

5. Junianto. 2003. Fish Handling Engineering. Penebar Swadaya, Jakarta, Indonesia. 58 pp.

6. King, W. and D.L. Berlinsky. 2006. Whole-body corticosteroid and plasma cortisol concentration in larval and juvenile Atlantic Cod Gadus morhua L. Folowing acute Stress. Aquaculture Research 37(13): 1282–1289.

7. Kubilay, A. and G. Ulukoy. 2002. The effect of acute stress on rainbow trout (Oncoryncus mykiss). Turkey Journal Zoology 26(2): 249–254.

8. Lim, L.C., P. Dhert and P. Sorgelos. 2003. Recent developments and improvements in ornamental fish packaging system for air transport. Aquaculture Research 34: 923–935.

9. Li, P., B. Ray, I.I.I.D.M. Gatlinn, T. Sink, R. Chen and R. Lochmann. 2009. Effect of handling and transport on cortisol response and nutrient mobilization of golden shiner, Notemigonus crysoleucas. Journal of the World Aquaculture Society 40: 803–809.

10. Lisdiana. 2012. Regulation of cortisol in stress and addiction. Biosaintifika Journal of Biology and Biology Education 4(1): 19–26. DOI: 10.15294/biosaintifika.v4i1.2264.

11. Madziga, H.A., S. Sanni and U.K. Sandabe. 2010. Phytochemical and elemental analysis of Acalypha wilkesiana Leaf. Journal of American Science 6: 510–514.

12. Martinez-Porchaz, M.P., L.R.M. Cordova and R.R. Enriquez. 2009. Corticol and glucose: riable indicators of fish stress?. Journal American of Aquatic Sciences 4: 158–178.

13. McEwen, B.S. and J.C. Wingfield. 2010. What’s in a name? Integrating homeostasis, allostasis and stress. Hormones and Behavior 57(2): 105–111.

14. Ortuño, J., M.A. Esteban and J. Meeguer. 2001. Effects of phenoxyethanol on the innate immune system of gilthead seabream (Sparus aurata L.) exposed to crowding stress. Veterinary Immunology and Immunopathology 89: 29–36.

15. Ortuño, J., M.A. Esteban and J. Meeguer. 2002. Effects of short-term crowding stress on the gilthead seabream (Sparus aurata L.) innate immune response. Fish and Shellfish Imumunology 11: 187–197. DOI: 10.1006/fsim.2000.0304

16. Ramsay, J.M., G.W. Feist, Z.M. Varga, M. Westerfield, M.L. Kent and C.B. Schreck. 2009. Whole-body cortisol response of zebrafish to acute net handling stress. Aquaculture 297(1-4): 157–162.

17. Ross, L.G. and B. Ross. 2008. Anesthetic and Sedative Techniques for Aquatic Animal, 3rd ed. Blackwell Publishing, London. 219 pp.

18. Salavati, S., A. Mogheiseh, S. Nazifi, A.S. Tabrizi, P. Taheri and F. Koohi. 2018. Changes in sexual hormones, serotonin, and cortisol concentrations following oral treatment of melatonin in castrated and intact dogs. Journal of Veterinary Behavior 27: 27–34.

19. Selmar, D., R. Liberei, B. Biehl and J. Voigt. 1987. Havea Linamarase-A Nonspecific β-Glycosidase. Journal Plant Physiology 83: 557–563.

20. Selye, H. 1936. A syndrome produced by diverse nocuous agents. Cited 26 Jan 2019.

21. Septiarusli, I.E., K. Haetami, Y. Mulyani and D. Danar. 2012. Potential of secondary metabolite compounds from keben (Barringtonia asiatica) seed extract in the anesthesia process of tiger grouper (Ephinephelus fuscoguttatus). Jurnal Perikanan dan Kelautan 3: 295–299.

22. Sink, T.D., R.T. Lochmann and K.A. Fecteu. 2008. Validation use and disadvantages of enzim-linked immunosorbent assay kits for detection of corticol in channel catfish, largemouth bass, red pacu and gold shiners. Fish Physiology and Biochemistry 34(1): 95–101. DOI: 10.1007/s10695-007-9150-9.

23. Sulmartiwi, L. 2014. Potential Essential Oil of Bandotan (A. conyzoides L.) as Reducing Substance Stress in Technology Transport Koi Fish (C. carpio L.). Disertasi. Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya. 1 p.

24. Tampubolon, R.S. 2012. Application of Imotilization Technique Using Macropomum Cuvier in Closed Transportation Systems with Stocking Solids 43, 86 dan 129 ekor/liter. Skripsi, Fakultas Teknologi dan Manajemen Akuakultur. Institut Pertanian Bogor, Bogor, Indonesia. 53 pp.

25. Velisek, J., Z. Svobodova and V. Piackova. 2005. Effects of clove oil anaesthesia on rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Brno 74: 139–146.

26. Wildan, A., I.A. Devina, H. Indah and Widayat. 2013. Oil extraction process from rubber seed solid waste with stirred extraction method. Momentum. Cited 23 May 2019.

27. World Wildlife Fund (WWF) Indonesia. 2015. Sea bass cultivation in floating net cage systems, 2nd ed. Better Management Practices: Seri Panduan Perikanan Skala Kecil. Cited 23 May 2019.