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Populations of seahorses (Hippocampus spp.) have been greatly reduced during recent decades due to over-exploitation, primarily from the ornamental fish and traditional Chinese medicine industries. Efforts at captive breeding have been made to reduce the dependence on wild-caught individuals, but to date, only some species have been bred successfully. The main obstacle in the culture of seahorses is the suitability of diets, as nutrient requirements of seahorses at different stages are not fully understood. Several studies have shown that Artemia enrichment improves the growth and survival of juvenile seahorses. This study determined the effect of feeding Artemia cultured with different media on growth and survival of Hippocampus barbouri. To prepare Artemia for the experiment, Artemia metanauplii were placed in cultured medium for 24 h, after sieved and rinsed, then another 30 min on respective cultured medium prior to feeding to seahorses. Five treatments were used: Artemia (A), Artemia with fresh Chlorella sp. (A+CF), Artemia with marine shrimp pellets (A+P), Artemia with Chlorella powder (A+CP) and Artemia with Spirulina powder (A+S). At the end of the experimental period, 74 days after birth, juveniles seahorse fed with diets A, A+P and A+S had significantly higher body length (p<0.05) (34.44±2.37 mm, 32.59±1.61 mm and 36.01±1.57 mm, respectively) than other treatments. A and A+S produced highest final weights (0.198±0.026 g and 0.221±0.057 g), while A+CF and A+CP produced lowest final weights. In terms of survival, diets A and A+S resulted in lowest (p<0.05) survival of 26.99 % as compared to 53.99 % in juveniles fed with A+CF, A+P and A+CP. To achieve better growth and higher survival, treatment A+P is highly recommended for rearing juvenile H. barbouri.
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2. Bhatnagar, A. and P. Devi. 2013. Water quality guidelines for the management of pond fish culture. International Journal of Environmental Sciences 3(6): 1980–2009.
3. Bengtson, D.A., P. Léger and P. Sorgeloos. 1991. Use of Artemia as a food source for aquaculture. Artemia Biology 11: 255–285.
4. Benijts, F., E. Vanvoorden and P. Sorgeloos. 1976. Changes in the biochemical composition of the early larval stages of the brine shrimp, Artemia salina L. Proceedings of the 10th European Symposium on Marine Biology 1: 1–9.
5. Boyd, C.E. and C.S. Tucker. 2012. Pond aquaculture water quality management. Springer Science+Business Media, New York. 700 pp.
6. Dhont, J., M. Wille, M. Frinsko, S.D. Coyle and P. Sorgellos. 2009. Larval feeds and feeding. In: Freshwater prawn biology and farming (eds. M.B. New, W.C. Valenti, J.H. Tidwell, L.R. D’Abramo and M.N. Kutty), pp. 86–103. John Wiley and Sons, Chichester, UK.
6. Dwiputra, A. 2013. Pemeliharaan juwana kuda laut (Hippocampus barbouri, Jordan and Richardson, 1908) dengan sistem resirkulasi. Ph.D. Thesis, Universitas Hasanuddin, Makassar, Indonesia. 40 pp.
7. Ferreira, M., P. Coutinho, P. Seixas, J. Fábregas and A. Otero. 2009. Enriching rotifers with “premium” microalgae. Nannochloropsis gaditana. Marine Biotechnology 11(5): 585–595.
8. Foster, S.A. and A.C.J. Vincent. 2004. Life history and ecology of seahorses: implications for conservation and management. Journal of Fish Biology 65(1): 1–61.
9. Hemaiswarya, S., R. Raja, R.R. Kumar, V. Ganesan and C. Anbazhagan. 2011. Microalgae: A sustainable feed source for aquaculture. World Journal of Microbiology and Biotechnology 27(8): 1737–1746.
10. Hilomen‐Garcia, G.V., R.D. Reyes and C.M.H. Garcia. 2003. Tolerance of seahorse Hippocampus kuda (Bleeker) juveniles to various salinities. Journal of Applied Ichthyology 19(2): 94–98.
11. Lourie, S. 2003. Measuring seahorses. Project seahorse technical report No.4. Project Seahorse, Fisheries Centre, University of British Columbia. Vancouver. 15 pp.
12. Mélo, R.C.S., L.P.D. Santos, A.P.M. Brito, A.D.A. Gouveia, C. Marcal and R.O. Cavalli. 2016. Use of the microalga Nannochloropsis occulata in the rearing of newborn longsnout seahorse Hippocampus reidi (Syngnathidae) juveniles. Aquaculture Research 47(12): 3934–3941.
13. Murugan, A., S. Dhanya., R.A. Sreepada., S. Rajagopal and T. Balasubramanian. 2009. Breeding and mass-scale rearing of three spotted seahorse, Hippocampus trimaculatus leach under captive conditions. Aquaculture 290(1–2): 87–96.
14. Muller-Feuga, A. 2000. The role of microalgae in aquaculture: situation and trends. Journal of Applied Phycology 12(3–5): 527–534.
15. Nur, F.A.H., A.R. Abdullah, Z.H. Muta and C.R. Saad. 2018. Effects of thyroxine, cod liver oil and potassium iodide on growth and survival of juvenile seahorse, Hippocampus barbouri. Aquaculture Research 49(2): 867–873.
16. Ornamental Aquatic Trade Association (OATA). 2008. Water quality criteria. Ornamental Aquatic Trade Association, Wiltshire, UK. 19 pp.
17. Olivotto, I., M.A. Avella, G. Sampaolesi, C.C. Piccinetti, P.N. Ruiz and O. Carnevali. 2008. Breeding and rearing the longsnout seahorse Hippocampus reidi rearing and feeding studies. Aquaculture 283(1–4): 92–96.
18. Ötleş, S. and R. Pire. 2001. Fatty acid composition of Chlorella and Spirulina microalgae species. Journal of AOAC International 84(6): 1708–1714.
19. Palma, J., D.P. Bureau and J.P. Andrade. 2011. Effect of different Artemia enrichments and feeding protocol for rearing juvenile long snout seahorse, Hippocampus guttulatus. Aquaculture 318(3–4): 439–443.
20. Payne, M.F. and R.J. Rippingale. 2000. Rearing West Australian seahorse, Hippocampus subelongatus, juveniles on copepod nauplii and enriched Artemia. Aquaculture 188(3–4): 353–361.
21. Planas, M., A. Chamorro, P. Quintas and A. Vilar. 2008. Establishment and maintenance of threatened long-snouted seahorse, Hippocampus guttulatus, broodstock in captivity.
Aquaculture 283(1): 19–28.
22. Sargent, J., G. Bell, L. McEvoy, D. Tocher and A. Estevez. 1999. Recent developments in the essential fatty acid nutrition of fish. Aquaculture 177(1–4): 191–199.
23. Sheng, J., Q. Lin, Q. Chen, Y. Gao, L. Shen and J. Lu. 2006. Effects of food, temperature and light intensity on the feeding behavior of three-spot juvenileseahorses, Hippocampus trimaculatus Leach. Aquaculture 256(1–4): 596–607.
24. Sorgeloos, P. 1980. The use of the brine shrimp Artemia in aquaculture. The brine shrimp Artemia 3: 25–46.
25. Sorgeloos, P., P. Dhert and P. Candreva. 2001. Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture 200(1–2): 147–159.
26. Vincent, A.C.J., S.J. Foster and H.J. Koldewey. 2011. Conservation and management of seahorses and other Syngnathidae. Journal of Fish Biology 78(6): 1681–1724.
27. Wong, J.M. and J.A.H. Benzie. 2003. The effects of temperature, Artemia enrichment, stocking density and light on the growth of juvenile seahorses, Hippocampus whitei (Bleeker, 1855), from Australia. Aquaculture 228(1–4): 107–121.
28. Woods, C.M. 2002. Natural diet of the seahorse Hippocampus abdominalis. New Zealand Journal of Marine and Freshwater Research 36(3): 655–660.
29. Woods, C.M. 2003. Growth and survival of juvenile seahorse Hippocampus abdominalis reared on live, frozen and artificial foods. Aquaculture 220(1–4): 287–298.
30. Woods, C.M. 2007. Aquaculture of the big-bellied seahorse Hippocampus abdominalis Lesson 1827 (Teleostei: Syngnathidae). Ph.D. Thesis, Victoria University of Wellington, Wellington, New Zealand. 238 pp.
31. Wilson, M.J. and A.C.J. Vincent. 2000. Preliminary success in closing the life cycle of exploited seahorse species, Hippocampus spp., in captivity. Aquarium Sciences and Conservation 2(4): 179–196.
32. Zaki, M.I. and H. Saad. 2010. Comparative study on growth and survival of larval and juvenile Dicentrarchus labrax rearing on rotifer and Artemia enriched with four different microalgae species. African Journal of Biotechnology 9(24): 3676–3688.