Effects of Dietary Supplementation with Broccoli Sprouts (Brassica oleracea) on the Hematology and Immunological Responses of Nile Tilapia (Oreochromis niloticus)

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Duangjai Pisuttharachai
Warrapong Nalinanon
Nontawith Areechon


We analyzed the effects of dietary administration of broccoli sprouts (Brassica oleracea) at varying concentrations (0, 10, 15, 20 and 25 g·kg-1 diet, for one and two weeks) on the hematology and select immunological parameters of Nile tilapia (Oreochromis niloticus). Subsequent results showed that the hematocrit level and red blood cell count remained unchanged following ingestion of broccoli sprouts for one and two weeks. However, the fish fed the supplemented diets of 20 and 25 g·kg-1 for two weeks displayed significant increase in white blood cell counts. Further analyses revealed that Nile tilapia fed with 10, 15, 20 and 25 g broccoli sprouts·kg-1 for two weeks displayed significant (p<0.05) improvement in phagocytic activity compared to that of the control group. In addition, serum bactericidal activity against Aeromonas hydrophila and Streptococcus agalactiae was enhanced in broccoli sprout-fed groups as early as one week after feeding. These results suggest that broccoli sprouts can enhance immune response in Nile tilapia by increasing total white blood cells and stimulating both cellular and humoral immunity.


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