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This study focused on isolation and characterisation of potential probiotic bacteria from a non–aquatic source (Gallus gallus domesticus). Enterococcus sp. was isolated from the midgut of G. gallus domesticus and was characterised for its ability to survive in artificial gastric juice, trypsin, varying pH and temperature, different concentrations of organic solvents, and was evaluated for its ammonia reduction potential. Notable inhibition of biofilm against Vibrio harveyi (41.0±3.2 %), Escherichia coli (33.0±4.0 %), Pseudomonas aeruginosa (37.8±4.0 %) and Staphylococcus aureus (41.7±2.0 %) was observed. The study showed that the isolate improved the survival rate of Danio rerio against V. harveyi and E. coli in challenge studies using survival analysis. The weight and length gains observed were 4.9±0.1 % and 0.3±0.2 % (p>0.05), respectively. The use of probiotics from non–aquatic sources can increase the diversity of available probiotics for aquaculture practices.
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