Effects of IgY Antibody on Growth, Survival, Immune Responses and Protection against Vibrio parahaemolyticus in Pacific White Shrimp

Main Article Content

Arunothai Keetanon
Niti Chuchird
Hae-Duck Bae
Mi-Kyoung Won
Su-Yeun Kim
Fazle Elahi

Abstract

Post larvae 12 (PL 12) of Litopenaeus vannamei (Pacific white shrimp) were subjected to feeding trials to decipher the effectiveness of IgY antibody as a feed supplement. This study aims to comprehend the growth performance, immune responses and survival rate of shrimps following an infection from Vibrio parahaemolyticus. This micro-organism was extracted from shrimps suffering from white feces syndrome (WFS). Post larvae (PL 12) were stocked in twenty 500-L fiberglass tanks at a density of 80 shrimps per tank (four replicate tanks per treatment) and salinity was maintained at 25 ‰. For analyses, shrimps were segregated into five categories based on the dosage of IgY antibody, given along with the standard pelleted shrimp feed (36 % protein) fed four times a day. This additive was fed at a dose escalation of 0, 0.2%, 0.3%, 0.4% and 0.5%. This 30-day trial demonstrated that the shrimps fed 0.5% of IgY additive had the highest average body weight. Besides this, shrimps nourished with 0.5% of IgY additive were found to have increases in growth and development. At the same time, a viable effect of antibody was also seen in the immunity of the shrimps, as evidenced by an escalation in immune responses such as total hemocyte count, phenoloxidase activity, phagocytosis and bactericidal activity of the shrimps. Along with this, a considerable mitigation of the total Vibrio spp. was found in the hepatopancreas of shrimps fed with IgY supplement. The shrimps infected with V. parahaemolyticus and furnished with IgY-feed were discovered to have a notable reduction in mortality rate (25-40 %) in contrast to the control group (60 %). This study demonstrates the positive effects of IgY on growth, immune responses and tolerance to pathogenic V. parahaemolyticus in the Pacific white shrimp.

Downloads

Download data is not yet available.

Article Details

Section
Articles

References

1. Arasteh, N., A.H. Aminirissehei, A.N. Yousif, L.J. Albright and T.D. Durance. 2004. Passive immunization of rainbow trout (Oncorhynchus mykiss) with chicken egg yolk immunoglobulins (IgY). Aquaculture 231(1): 23-36.

2. Baloch, A.R., X.Y. Zhang and R. Schade. 2015. IgY technology in aquaculture – a review. Reviews in Aquaculture 7: 153-160.

3. Chuchird, N., P. Rorkwiree and T. Rairat. 2015. Effect of dietary formic acid and astaxanthin on the survival and growth of Pacific white shrimp (Litopenaeus vannamei) and their resistance to Vibrio parahaemolyticus. SpringerPlus 4: 440. DOI: 10.1186/s40064-015-1234-x.

4. de Paula, V.S., A.D. Silva, G.A. Vasconcelos, E.T. Iff, M.E. Vasconcelos, L.A. Kappel, P.B. Cruz and M.A. Pinto. 2011. Applied biotechnology for production of immunoglobulin Y specific to hepatitis A virus. Journal of Virological Methods 171: 102-106.

5. Gao, X., X. Zhang, L. Lin, D. Yao, J. Sun, X. Du, X. Li and Y. Zhang. 2016. Passive immune-protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus infections with anti-Vibrio egg yolk (IgY) encapsulated feed. The International Journal of Molecular Science 17: 1-10.

6. Hu, B., X. Yang, E. Guo, P. Zhou, D. Xu, Z. Qi and L. Deng. 2018. The preparation and antibacterial effect of egg yolk immunoglobulin (IgY) against the outer membrane proteins of Vibrio parahaemolyticus. Journal of the Science of Food and Agriculture 99(5): 2565-2571.

7.Johnson, S.K. 1995. Handbook of shrimp diseases. Texas A&M University Sae Grant College Program, College Station, Texas. 150 pp.

8. Jueliang, P., N. Chuchird and C. Limsuwan. 2013. The effects of probiotic, B-1, 3-glucan and organic acid on Pacific white shrimp's (Litopenaeus vanamei) immune system and survival upon challenge with Vibrio harveyi. Kasetsart University Fisheries Research Bulletin 37(3): 25-37.

9. Kumaran, T., E. Thirumalaikumar, C. Lelin, P. Palanikumar, M. Michaelbabu and T. Citarasu. 2018. Physicochemical properties of anti Vibrio harveyi egg yolk antibody (IgY) and its immunological influence in Indian white shrimp Fenneropenaeus indicus. Fish and Shellfish Immunology 74: 349-362.

10. Li, C.H., L.X. Jiang, L.D. Feng and C. Jiong. 2014. Passive protective effect of chicken egg yolk immunoglobulins against experimental Vibrio anguillarum infection in ayu (Plecoglossus altivelis). Fish and Shellfish Immunology 37(1): 108-114.

11. Li, X., K. Jing, X. Wang, Y. Li, M. Zhang, Z. Li, L. Xu, L. Wang and Y. Xu. 2016. Protective effects of chicken egg yolk antibody (IgY) against experimental Vibrio splendidus infection in the sea cucumber (Apostichopus japonicus). Fish and Shellfish Immunology 43: 105-111.

12. Lu, Y., L. Junjun, J. Liji, L. Xiaoyu, Z.Y. Hong, X. Hongyu and X. Yongping. 2008. Passive immunization of crayfish (Procambius clarkiaii) with chicken egg yolk immunoglobulin (IgY) against white spot syndrome virus (WSSV). Journal of Biotechnology 136: 144-152.

13. Mine, Y. and J. Kovacs-Nolan. 2002. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. Journal of Medicinal Food 5: 159-169.

14. Nayak, S., C. Limsuwan, N. Chuchird and S. Pungpang. 2012. A study on the effect of Bacillus spp. to control the pathogenic bacteria in aquaculture. Kasetsart University Fisheries Research Bulletin 36(2): 1-13.

15. Nonwachai, T., W. Purivirojkul, C. Limsuwan, N. Chuchird, M. Velasco and A.K. Dhar. 2010. Growth, nonspecific immune characteristics, and survival upon challenge with Vibrio harveyi in Pacific white shrimp (Litopenaeus vannamei) raised on diets containing algal meal. Fish and Shellfish Immunology 29(2): 298-304.

16. Somboon, M., W. Purivirojkul, C. Limsuwan and N. Chuchird. 2012. Effect of Vibrio spp. in white feces infected shrimp in Chanthaburi, Thailand. Kasetsart University Fisheries Research Bulletin 36(1): 7-15.

17. Sriurairatana, S., V. Boonyawiwat, W. Gangnonngiw, C. Laosutthipong, J. Hiranchan and T.W. Flegel. 2014. White feces syndrome of shrimp arises from transformation, sloughing and aggregation of hepatopancreatic microvilli into vermiform bodies superficially resembling gregarines. Public Library of Science 9(6): e99170. DOI: 10.1371/journal.pone.0099170.

18. Supono, W., E. Harpeni, A.H. Khotimah and A. Ningtyas. 2019. Identification of Vibrio sp. as cause of white feces diseases in white shrimp Penaeus vannamei and handling with herbal ingredients in East Lampung Regency, Indonesia AACL Bioflux 12: 417-425.

19. Thitamadee, S., A. Prachumwat, J. Srisala, P. Jaroenlak, P.V. Salachan, K. Sritunyalucksana, T.W. Flegel and O. Itsathitphaisarn. 2016. Review of current disease threats for cultivated penaeid shrimp in Asia. Aquaculture 452: 69-87.

20. Walla, W., W. Purivirojkul, N. Chuchird and C. Limsuwan. 2011. Effect of activate on growth, survival and the total number of bacteria and Vibrio spp. in rearing of Pacific white shrimp (Litopenaeus vannamei). Kasetsart University Fisheries Research Bulletin 32(2): 14-22.

21. Wu, C.J., H. Wang, Y.L. Chan and T.L. Li. 2011. Passive immune-protection of small abalone against Vibrio alginolyticus infection by anti-Vibrio IgY-encapsulated feed. Fish and Shellfish Immunology 30: 1042-1048.

22. Xu, L., Y. Xu, L. He, M. Zhang, L. Wang, Z. Li and X. Li. 2019. Immunomodulatory effects of chicken egg yolk antibodies (IgY) against experimental Shewanella marisflavi AP629 infections in sea cucumbers (Apostichopus japonicus). Fish and Shellfish Immunology 84: 108-119.

23. Xu, Y., X. Li, L. Jin, Y. Zhen, Y. Lu, S. Li, J. You and L. Wang. 2011. Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: A review. Biotechnology Advances 29(6): 860-868.

24. Zhang, Q., H. Lin, J.X. Sui, J.X. Wang and L.M. Cao. 2015. Effects of Fab' fragments of specific egg yolk antibody (IgY-Fab′) against Shewanella putrefaciens on the preservation of refrigerated turbot. Journal of the Science of Food and Agriculture 95: 136-140.

25. Zhang, Y., F. Yan, Z. Hu, X. Zhao, S. Min and Z. Du. 2009. Hemocyanin from shrimp Litopenaeus vannamei shows hemolytic activity. Fish and Shellfish Immunology 27: 330-335.

26. Zhen, Y., J.L. Ji, G. Jie, L.X. Yu, L.Y. Nan, C. Jie, and X.Y. Ping. 2008. Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Escherichia coli. Veterinary Microbiology 130(1): 126-133.