Mitochondrial DNA Analysis Suggests Invasion of Thailand Coast by a Single Species of Dreissenidae, Mytilopsis sallei
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Published:
Nov 1, 2021
Keywords:
Alien invasive species COI gene False mussels Mytilopsis Thailand
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Abstract
In 2001, a species of false mussel was recorded in coastal areas in southern Thailand. In 2008, established populations were reported in several estuaries and lagoons. Based on shell morphology, the bivalve was identified as a non-native species belonging to Dreissenidae. To date, populations have been observed in five locations in southern Thailand on both the Andaman and Gulf of Thailand coasts. The variation in shell morphology within and between these locations led us to question whether specimens in different locations belonged to different species. We used morphometric techniques to investigate variation in shell characteristics among the five locations and mtDNA (COI) analysis to investigate genetic variation. Shell characteristics varied among locations; the shell morphology of specimens from some locations on the Andaman coast appeared to be similar to that of specimens from the Gulf coast. Most specimens had long, prominent apophyses, but in some specimens they were rounded and not prominent. In spite of the high variation in shell morphology, molecular analysis indicated that all COI gene sequences of the specimens examined are nearly identical and belong to a single species, Mytilopsis sallei. Phylogenetic analyses also revealed the monophyly of 25 sequences between our specimens and other M. sallei sequences from GenBank, with high bootstrap support. Our recent findings suggest that the name M. sallei should be applied to the false mussel in southern Thailand. However, this merits confirmation with analysis using other molecular markers, and more specimens should be included.
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Klangnurak, W. ., & Wangkulangkul, K. . (2021). Mitochondrial DNA Analysis Suggests Invasion of Thailand Coast by a Single Species of Dreissenidae, Mytilopsis sallei. Journal of Fisheries and Environment, 45(3), 64–76. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/252838
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References
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19. Morton, B. 1981. The biology and functional morphology of Mytilopsis sallei (Recluz) (Bivalvia: Dreissenacea) fouling Visakhapatnam Harbour, Andhra Pradesh, India. Journal of Molluscan Studies 47(1): 25-42.
20. Owada, M., Y. Kasai, S.I. Sato and J.S. Hong. 2013. Shell morphology and genetic characteristics of Corbula (Potamocorbula) spp. (Bivalvia: Corbulidae) from the waters adjacent to Japan, Korea and San Francisco Bay. Aquatic Biology 19(3): 297-305.
21. Pathy, D.A. and G.L. Mackie. 1993. Comparative shell morphology of Dreissena polymorpha, Mytilopsis leucophaeata, and the" quagga" mussel (Bivalvia: Dreissenidae) in North America. Canadian Journal of Zoology 71(5): 1012-1023.
22. Pavlova, V. 2012. First finding of deepwater profunda morph of quagga mussel Dreissena bugensis in the European part of its range. Biological Invasions 14(3): 509-514.
23. Peyer, S.M., J.C. Hermanson and C.E. Lee. 2010. Developmental plasticity of shell morphology of quagga mussels from shallow and deep-water habitats of the Great Lakes. Journal of Experimental Biology 213(15): 2602-2609.
24. Pongparadon, S., G.C. Zuccarello, S.M. Phang, H. Kawai, T. Hanyuda and A. Prathep. 2015. Diversity of Halimeda (Chlorophyta) from the Thai-Malay Peninsula. Phycologia 54(4):
349-366.
25. Posada, D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25: 1253-1256.
26. Raju, P.R., K.M. Rao, S.S. Ganti and N. Kalyanasundaram. 1975. Effect of extreme salinity conditions on the survival of Mytilopsis sallei Recluz (Pelecypoda). Hydrobiologia 46(2-3): 199-206.
27. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York. 545 pp.
28. Sa-Nguansil, S. and K. Wangkulangkul. 2020. Salinity tolerance in different life history stages of an invasive false mussel Mytilopsis sallei Recluz, 1849: implications for its restricted distribution. Molluscan Research 40(3): 214-222.
29. Swennen, C., R.G. Moolenbeek, N. Ruttanadakul, H. Hobbelink, H. Dekker and S. Hajisamae. 2001. The Molluscs of the Southern Gulf of Thailand. Biodiversity Research and Training Program (BRT), Bangkok. 210 pp.
30. Tan, K.S. and T. Tay. 2018. The invasive Caribbean Mytilopsis sallei (Bivalvia: Dreissenidae): A short review. ASEAN Journal on Science and Technology for Development 35(1-2): 133-139.
31. Therriault, T.W., M.F. Docker, M.I. Orlova, D.D. Heath and H.J. MacIsaac. 2004. Molecular resolution of the family Dreissenidae (Mollusca: Bivalvia) with emphasis on Ponto-Caspian species, including first report of Mytilopsis leucophaeata in the Black Sea basin. Molecular Phylogenetics and Evolution 30(3): 479-489.
32. Trichkova, T.A., D.S. Kozuharov, Z.K. Hubenov, I.S. Botev, M.T. Zivkov and S.D. Cheshmedjiev. 2008. Characteristics of zebra mussel (Dreissena polymorpha) populations in infested reservoirs, northwest Bulgaria. Journal of Natural History 42(5-8): 619-631.
33. Van der Gaag, M., G. Van der Velde, S. Wijnhoven and R.S. Leuven. 2016. Salinity as a barrier for ship hull-related dispersal and invasiveness of dreissenid and mytilid bivalves. Marine Biology 163(7): 147. DOI: 10.1007/s00227-016-2926-7.
34. Verween, A., M. Vincx and S. Degraer. 2010. Mytilopsis leucophaeata: the brackish water equivalent of Dreissena polymorpha? a review. In: The Zebra Mussel in Europe (eds. G. Van der Velde, S. Rajagopal and A. Bij de Vaate), pp. 29-44. Backhuys Publishers, Leiden and Margraf Publishers, Weikersheim.
35. Wangkulangkul, K. and V. Lheknim. 2008. The occurrence of an invasive alien mussel Mytilopsis adamsi Morrison, 1946 (Bivalvia: Dreissenidae) in estuaries and lagoons of the lower south of the Gulf of Thailand with comments on their establishment. Aquatic Invasions 3(3): 325-330.
36. Wangkulangkul, K. 2018. Comments on restricted distribution of Mytilopsis adamsi Morrison, 1946, a non-native false mussel in the Songkhla Lagoon System, southern Thailand. Limnology 19(1): 151-156.
37. Wells, F.E. 2019. Environmental emergency: why did the false mussel Mytilopsis sallei not invade Darwin Harbour, Australia? Malacologia 62 (2): 247-256.
38. Willan, R.C., B.C. Russell, N.B. Murfet, K.L. Moore, F.R. McEnnulty, S.K. Horner, C.L. Hewitt, G.M. Dally, M.L. Campbell and S.T. Bourke. 2000. Outbreak of Mytilopsis sallei (Récluz, 1849) (Bivalvia: Dreissenidae) in Australia. Molluscan Research 20(2): 25-30.
39. Wong, Y.T., R. Meier and K.S. Tan. 2011. High haplotype variability in established Asian populations of the invasive Caribbean bivalve Mytilopsis sallei (Dreissenidae). Biological Invasions 13: 341-348.
Balkans. Freshwater Biology 52(8): 1525-1536.
2. Altschul, S.F., T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research 25(17): 3389-3402.
3. Alunno-Bruscia, M., E. Bourget and M. Fréchette. 2001. Shell allometry and length-mass-density relationship for Mytilus edulis in an experimental food-regulated situation. Marine Ecology Progress Series 219: 177-188.
4. Anderson, M.J., R.N. Gorley and K.R. Clarke. 2008. PERMANOVA+for Primer: Guide to Software and Statistical Methods. PRIMER-E, Ltd., Plymouth. 214 pp.
5. Beggel, S., A. Cerwenka, J. Brandner and J. Geist. 2015. Shell morphological versus genetic identification of quagga mussel (Dreissena bugensis) and zebra mussel (Dreissena polymorpha). Aquatic Invasions 10(1): 93-99.
6. Bilandzija, H., B. Morton, M. Podnar and H. Ćetković. 2013. Evolutionary history of relict Congeria (Bivalvia: Dreissenidae): unearthing the subterranean biodiversity of the Dinaric Karst. Frontiers in Zoology 10(1): 5. DOI: 10.1186/1742-9994-10-5.
7. Fernandes, M.R., F. Salgueiro, I.C. Miyahira and C.H.S. Caetano. 2018. mtDNA analysis of Mytilopsis (Bivalvia, Dreissenidae) invasion in Brazil reveals the existence of two species. Hydrobiologia 817(1): 97-110.
8. Gelembiuk, G.W., G.E. May and C.E. Lee. 2006. Phylogeography and systematics of zebra mussels and related species. Molecular Ecology 15(4): 1033-1050.
9. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
10. He, J., J.F. Qi, D.Q. Feng and C.H. Ke. 2016. Embryonic and larval development of the invasive biofouler Mytilopsis sallei (Récluz, 1849) (Bivalvia: Dreissenidae). Journal of Molluscan Studies 82(1): 23-30.
11. Hebert, P.D., B.W. Muncaster and G.L. Mackie. 1989. Ecological and genetic studies on Dreissena polymorpha (Pallas): a new mollusc in the Great Lakes. Canadian Journal of Fisheries and Aquatic Sciences 46(9): 1587-1591.
12. Heiler, K.C., N. Nahavandi and C. Albrecht. 2010. A new invasion into an ancient lake-the invasion history of the dreissenid mussel Mytilopsis leucophaeata (Conrad, 1831) and its first record in the Caspian Sea. Malacologia 53(1): 185-192.
13. Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35(6): 1547-1549.
14. Lajtner, J., Z. Marusic, G.I.V. Klobucar, I. Maguire and R. Erben. 2004. Comparative shell morphology of the zebra mussel, Dreissena polymorpha in the Drava river (Croatia). Biologia, Bratislava 59(5): 595-600.
15. Lodeiros, C., N. González-Henríquez, J. Cuéllar-Anjel, D. Hernández-Reyes, C. Medina- Alcaraz, J. Quinteiro and M. Rey-Méndez. 2019. Invasion of the dark false mussel in shrimp farms in Venezuela: species identification and genetic analysis. Bioinvasions Records 8: 838-847.
16. Lutaenko, K.A., L.A. Prozorova, X.Q. Ngo and V.V. Bogatov. 2019. First reliable record of Mytilopsis sallei (Récluz, 1849) (Bivalvia: Dreissenidae) in Vietnam. The Korean Journal of Malacology 35(4): 355-360.
17. Marelli, D.C. and S. Gray. 1983. Conchological redescriptions of Mytilopsis sallei and Mytilopsis leucophaeta of the brackish Western Atlantic. The Veliger 25(3): 185-193.
18. Mohan, P.C. and R.R. Prakash. 1998. Concentration of petroleum hydrocarbons in bivalve Mytilopsis sallei and in the harbour waters of Visakhapatnam, east coast of India. Indian Journal of Geo-Marine Sciences 27(3-4): 496-498.
19. Morton, B. 1981. The biology and functional morphology of Mytilopsis sallei (Recluz) (Bivalvia: Dreissenacea) fouling Visakhapatnam Harbour, Andhra Pradesh, India. Journal of Molluscan Studies 47(1): 25-42.
20. Owada, M., Y. Kasai, S.I. Sato and J.S. Hong. 2013. Shell morphology and genetic characteristics of Corbula (Potamocorbula) spp. (Bivalvia: Corbulidae) from the waters adjacent to Japan, Korea and San Francisco Bay. Aquatic Biology 19(3): 297-305.
21. Pathy, D.A. and G.L. Mackie. 1993. Comparative shell morphology of Dreissena polymorpha, Mytilopsis leucophaeata, and the" quagga" mussel (Bivalvia: Dreissenidae) in North America. Canadian Journal of Zoology 71(5): 1012-1023.
22. Pavlova, V. 2012. First finding of deepwater profunda morph of quagga mussel Dreissena bugensis in the European part of its range. Biological Invasions 14(3): 509-514.
23. Peyer, S.M., J.C. Hermanson and C.E. Lee. 2010. Developmental plasticity of shell morphology of quagga mussels from shallow and deep-water habitats of the Great Lakes. Journal of Experimental Biology 213(15): 2602-2609.
24. Pongparadon, S., G.C. Zuccarello, S.M. Phang, H. Kawai, T. Hanyuda and A. Prathep. 2015. Diversity of Halimeda (Chlorophyta) from the Thai-Malay Peninsula. Phycologia 54(4):
349-366.
25. Posada, D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25: 1253-1256.
26. Raju, P.R., K.M. Rao, S.S. Ganti and N. Kalyanasundaram. 1975. Effect of extreme salinity conditions on the survival of Mytilopsis sallei Recluz (Pelecypoda). Hydrobiologia 46(2-3): 199-206.
27. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York. 545 pp.
28. Sa-Nguansil, S. and K. Wangkulangkul. 2020. Salinity tolerance in different life history stages of an invasive false mussel Mytilopsis sallei Recluz, 1849: implications for its restricted distribution. Molluscan Research 40(3): 214-222.
29. Swennen, C., R.G. Moolenbeek, N. Ruttanadakul, H. Hobbelink, H. Dekker and S. Hajisamae. 2001. The Molluscs of the Southern Gulf of Thailand. Biodiversity Research and Training Program (BRT), Bangkok. 210 pp.
30. Tan, K.S. and T. Tay. 2018. The invasive Caribbean Mytilopsis sallei (Bivalvia: Dreissenidae): A short review. ASEAN Journal on Science and Technology for Development 35(1-2): 133-139.
31. Therriault, T.W., M.F. Docker, M.I. Orlova, D.D. Heath and H.J. MacIsaac. 2004. Molecular resolution of the family Dreissenidae (Mollusca: Bivalvia) with emphasis on Ponto-Caspian species, including first report of Mytilopsis leucophaeata in the Black Sea basin. Molecular Phylogenetics and Evolution 30(3): 479-489.
32. Trichkova, T.A., D.S. Kozuharov, Z.K. Hubenov, I.S. Botev, M.T. Zivkov and S.D. Cheshmedjiev. 2008. Characteristics of zebra mussel (Dreissena polymorpha) populations in infested reservoirs, northwest Bulgaria. Journal of Natural History 42(5-8): 619-631.
33. Van der Gaag, M., G. Van der Velde, S. Wijnhoven and R.S. Leuven. 2016. Salinity as a barrier for ship hull-related dispersal and invasiveness of dreissenid and mytilid bivalves. Marine Biology 163(7): 147. DOI: 10.1007/s00227-016-2926-7.
34. Verween, A., M. Vincx and S. Degraer. 2010. Mytilopsis leucophaeata: the brackish water equivalent of Dreissena polymorpha? a review. In: The Zebra Mussel in Europe (eds. G. Van der Velde, S. Rajagopal and A. Bij de Vaate), pp. 29-44. Backhuys Publishers, Leiden and Margraf Publishers, Weikersheim.
35. Wangkulangkul, K. and V. Lheknim. 2008. The occurrence of an invasive alien mussel Mytilopsis adamsi Morrison, 1946 (Bivalvia: Dreissenidae) in estuaries and lagoons of the lower south of the Gulf of Thailand with comments on their establishment. Aquatic Invasions 3(3): 325-330.
36. Wangkulangkul, K. 2018. Comments on restricted distribution of Mytilopsis adamsi Morrison, 1946, a non-native false mussel in the Songkhla Lagoon System, southern Thailand. Limnology 19(1): 151-156.
37. Wells, F.E. 2019. Environmental emergency: why did the false mussel Mytilopsis sallei not invade Darwin Harbour, Australia? Malacologia 62 (2): 247-256.
38. Willan, R.C., B.C. Russell, N.B. Murfet, K.L. Moore, F.R. McEnnulty, S.K. Horner, C.L. Hewitt, G.M. Dally, M.L. Campbell and S.T. Bourke. 2000. Outbreak of Mytilopsis sallei (Récluz, 1849) (Bivalvia: Dreissenidae) in Australia. Molluscan Research 20(2): 25-30.
39. Wong, Y.T., R. Meier and K.S. Tan. 2011. High haplotype variability in established Asian populations of the invasive Caribbean bivalve Mytilopsis sallei (Dreissenidae). Biological Invasions 13: 341-348.