High Genetic Diversity and Gene Flow among Cultured and Wild Populations of Bighead Catfish (Clarias macrocephalus) in the Mekong Delta of Viet Nam Inferred from ISSR Markers
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Abstract
Bighead catfish (Clarias macrocephalus) is an important aquaculture species in the Mekong Delta of Viet Nam, but its farming has been facing such difficulties as slow growth and low seed quality. To apply genetic improvement programs to enhance farming production, it is crucial to start with a base population with high genetic diversity. This study compared genetic diversity between cultured and wild bighead catfish populations using inter-simple sequence repeat (ISSR) markers. Samples were collected in the Mekong Delta from two cultured populations (in Can Tho and Long An provinces) and two wild populations (in Ca Mau and Hau Giang). A total of 112 individuals was screened using six highly polymorphic ISSR primers which generated 61 scorable bands (500-3,000 bp). The study found that levels of genetic diversity of bighead catfish populations were high, with the number of effective alleles from 1.42 to 1.50, Shannon Index from 0.381 to 0.433, and unbiased expected heterozygosity from 0.257 to 0.297; those values from cultured populations were lower than wild populations. Moreover, the value of Nei’s unbiased genetic distance among populations was low, indicating high gene flow among bighead catfish populations distributed in the Mekong Delta, Viet Nam.
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References
Bi, X., Q. Yang, T. Gao and C. Li. 2011. The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: Implications for its conservation. Chinese Journal of Oceanology and Limnology 29: 958-966. DOI: 10.1007/s00343-011-0185-5.
Dunham, R.A. 2011. Aquaculture and Fisheries Biotechnology: Genetic Approaches, 2nd ed. CABI Publishing, Oxfordshire, UK. 503 pp.
Duong, T.Y. and K.T. Scribner. 2018. Regional variation in genetic diversity between wild and cultured populations of bighead catfish (Clarias macrocephalus) in the Mekong Delta. Fisheries Research 207: 118-125. DOI: 10.1016/j.fishres.2018.06.012.
Duong, T.Y. and T.V. Nguyen. 2019. Genetic diversity of Pangasius krempfi in the Mekong River estuaries. Can Tho University Journal of Sciene 11: 81-88. DOI: 10.22144/ctu.jen.2019.027.
Duong, T.Y., K.T. Scribner, J. Kanefsky and U. Na-Nakorn. 2017. Lack of introgressive hybridization by North African catfish (Clarias gariepinus) in native Vietnamese bighead catfish (Clarias macrocephalus) populations as revealed by novel nuclear and mitochondrial markers. Aquaculture 473: 468-477. DOI: 10.1016/j.aquaculture.2017.03.007.
Duong, T.Y., T.N.T. Nguyen, T.T. Nguyen, T.T.L. Huynh, M.T. Bui, T.L. Pham and R. Dunham. 2022. Growth and survival of crossbreeds between wild and domestic strains of Mekong Delta bighead catfish (Clarias macrocephalus Günther, 1864) cultured in tanks. Aquaculture 547: 737507. DOI: 10.1016/j.aquaculture.2021.737507.
Fernandes-Matioli, F.M.C., S.R. Matioli and L.E. Almeida-Toledo. 2000. Species diversity and geographic distribution of Gymnotus (Pisces: Gymnotiformes) by nuclear (GGAC)n microsatellite analysis. Genetics and Molecular Biology 23(4): 803-807. DOI: 10.1590/S1415-47572000000400016.
Ford, J.S. and R.A. Myers. 2008. A global assessment of salmon aquaculture impacts on wild salmonids. PLoS Biology 6: 411-417. DOI: 10.1371/journal.pbio.0060033.
Frost, L.A., B.S. Evans and D.R. Jerry. 2006. Loss of genetic diversity due to hatchery culture practices in barramundi (Lates calcarifer). Aquaculture 261: 1056-1064. DOI: 10.1016/j.aquaculture.2006.09.004.
Hayes, B., J. He, T. Moen and J. Bennewitz. 2006. Use of molecular markers to maximise diversity of founder populations for aquaculture breeding programs. Aquaculture 255: 573-578. DOI: 10.1016/j.aquaculture.2005.11.038.
Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7): 1870-1874. DOI: 10.1093/molbev/msw054.
Kumla, S., S. Doolgindachbaporn, R. Sudmoon and N. Sattayasai. 2012. Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Reports 39: 5201-5210. DOI: 10.1007/s11033-011-1317-x.
Labastida, E., D. Cobián, Y. Hénaut, M. del Carmen García-Rivas, P.P Chevalier and S. MacHkour-M’rabet. 2015. The use of ISSR markers for species determination and a genetic study of the invasive lionfish in Guanahacabibes, Cuba. Latin American Journal of Aquatic Research 43(5): 1011-1018. DOI: 10.3856/vol43-issue5-fulltext-2.
Li, W., W.X. Sun, J. Fan and C.C. Zhang. 2013. Genetic diversity of wild and cultured swamp eel (Monopterus albus) populations from central China revealed by ISSR markers. Biologia 68: 727-732. DOI: 10.2478/s11756-013-0203-5.
Muiocha, D.A., S. Onming and U. Na-Nakorn. 2017. Growth performance, genetic diversity and morphometric traits of an introduced wild and hatchery population of Clarias macrocephalus Günther, 1864. Journal of Fisheries and Environment 41: 1-19.
Na-Nakorn, U. 2004. A perspective on breeding and genetics of walking catfish in Thailand. Aquaculture Asia 9: 10-12.
Na-Nakorn, U. and T. Moeikum. 2009. Genetic diversity of domesticated stocks of striped catfish, Pangasianodon hypophthalmus (Sauvage 1878), in Thailand: Relevance to broodstock management regimes. Aquaculture 297: 70-77. DOI: 10.1016/j.aquaculture.2009.09.014.
Na-Nakorn, U., W. Kamonrat and T. Ngamsiri. 2004. Genetic diversity of walking catfish, Clarias macrocephalus, in Thailand and evidence of genetic introgression from introduced farmed C. gariepinus. Aquaculture 240: 145-163. DOI: 10.1016/j.aquaculture.2004.08.001.
Nazia, A.K., B.M. Tam, J.A.F. Jamaluddin and S.A. Mohd Nor. 2021. High genetic structure between natural populations of bighead catfish Clarias macrocephalus (Günther, 1864) from the Mekong Delta and Peninsular Malaysia. Fisheries Research 241: 105993. DOI: 10.1016/j.fishres.2021.105993.
Oliveira, C. de S.T., R.F.C. Moreira, A.A. Soares Filho, S.B.A. Fonteles and N.S. Evangelista-Barreto. 2019. Genetic diversity in natural populations of Colossoma macropomum in the Brazilian Amazon region and in populations farmed in northeast Brazil based on ISSR markers. Aquaculture International 27: 1423-1434. DOI: 10.1007/s10499-019-00395-1.
Pazza, R., K.F. Kavalco, S.M.A.P. Prioli, A.J. Prioli and L.A.C. Bertollo. 2007. Chromosome polymorphism in Astyanax fasciatus (Teleostei, Characidae), Part 3: Analysis of the RAPD and ISSR molecular markers. Biochemical Systematics and Ecology 35: 843-851. DOI: 10.1016/J.BSE.2007.03.018.
Peakall, R. and P.E. Smouse. 2012. GenALEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-An update. Bioinformatics 28: 2537-2539. DOI: 10.1093/bioinformatics/bts460.
Pechsiri, J. and A. Vanichanon. 2016. Genetic diversity in slender walking catfish (Clarias nieuhofii) populations: Implications for population management. Walailak Journal of Science and Technology 13: 511-519. DOI: 10.14456/vol13iss6pp%p.
Raghuwanshi, K.S., B.A. Hujare, V.P. Chimote and S.G. Borkar. 2013. Characterization of Xanthomonas axonopodis pv. punicae isolates from Western Maharashtra and their sensitivity to chemical treatments. The Bioscan 8 (3): 845-850.
Saad, Y.M., M.A. Rashed, A.H. Atta and N.E. Ahmed. 2012. Genetic diversity among some tilapia species based on ISSR markers. Life Science Journal 9(4): 4841-4846.
Saporito-Irwin, S.M., T. Geist and D.H. Gutmann. 1997. Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections. Biotechniques 23: 424-427. DOI: 10.2144/97233bm16.
Sawasawa, W. and T.Y. Duong. 2020. Genetic diversity of endangered snakehead Channa lucius (Cuvier, 1831) in the Mekong Delta inferred from ISSR markers. Asian Fisheries Science 33: 266-273. DOI: 10.33997/j.afs.2020.33.3.008.
Saxena, A., R. Raghuwanshi and H.B. Singh. 2014. Molecular, phenotypic and pathogenic variability in Colletotrichum isolates of subtropical region in north-eastern India, causing fruit rot of chillies. Journal Applied Microbioly 117: 1422-1434. DOI: 10.1111/jam.12607.
Schönhuth, S., G. Luikart and I. Doadrio. 2003. Effects of a founder event and supplementary introductions on genetic variation in a captive breeding population of the endangered Spanish killifish. Journal of Fish Biology 63: 1538-1551. DOI: 10.1111/J.1095-8649.2003.00265.X.
Sharma, S.K., S. Kumaria, P. Tandon and S.R. Rao. 2011. Single primer amplification reaction (SPAR) reveals inter- and intra-specific natural genetic variation in five species of Cymbidium (Orchidaceae). Gene 483: 54-62. DOI: 10.1016/j.gene.2011.05.013.
Tave, D. 1993. Genetics for Fish Hatchery Managers, 2nd ed. Van Nostrand Reinhold, New York, USA. 432 pp.
Tave, D. 1999. Inbreeding and Broodstock Management. Food and Agriculture Organization of the United Nations, Rome, Italy. 122 pp.
Wachirachaikarn, A., W. Rungsin, P. Srisapoome and U. Na-Nakorn. 2009. Crossing of African catfish, Clarias gariepinus (Burchell, 1822), strains based on strain selection using genetic diversity data. Aquaculture 290: 53-60. DOI: 10.1016/j.aquaculture.2009.01.036.
Yeh, F., R. Yang and T. Boyle. 1999. Popgene Version 1.3.1. Mircosoft Window-Based Freeware for Population Genetic Ananlysis. University of Alberta, Edmonton, Canada. 29 pp.
Zhou, Y., J. Tong, J. Wang and X. Yu. 2020. Development of microsatellite markers and genetic diversity in wild and cultured populations of black carp (Mylopharyngodon piceus) along the Yangtze River. Aquaculture International 28: 1867-1882. DOI: 10.1007/s10499-020-00563-8.