The Bactericidal Effect of Essential Oils in Vietnam to Vibrio parahaemolyticus Causing AHPND in Shrimp
Article Sidebar
Main Article Content
Abstract
Ocimum gratissimum (clove basil), Cymbopogon citratus (lemongrass), and Mentha arvensis (corn mint) are common Vietnamese herbs with great antibacterial activity. This study was conducted to showcase the feasibility of utilizing these herbs for disease management in aquaculture, shrimp farming in particular. Firstly, these herbs’ components were identified by gas chromatography-mass spectrometry (GC-MS). Their antimicrobial activity was then assessed against three strains of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (AHPND) in shrimp with three colony types on CHROMagar Vibrio by the disc diffusion method, and the minimum inhibitory concentration (MIC) values were determined. The results showed that the three studied bacterial strains were sensitive to three tested essential oils. VE1 strain, which formed type 1 colony on CHROMagar Vibrio, was the most sensitive to corn mint, lemongrass and clove basil essential oils with MIC values of 0.16%, 0.08%, and 0.08% (v/v), respectively. Meanwhile, MIC values of the aforementioned essential oils in the strains forming type 2 and 3 colonies (VE2 and VE3) were 0.32%, 0.08%, and 0.16% (v/v), respectively. These results indicate a potential application of lemongrass, corn mint and clove basil essential oils in AHPND disease control in shrimp. In order to increase the applicability of these essential oils, further studies on their synthesis in micro-emulsions and evaluation of their effectiveness should be carried out.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Adila, N.H., B. Anto and N.J. Siti. 2018. Detection of tlh and tdh genes in Vibrio parahaemolyticus inhabiting farmed water ecosystem used for L. vannamei aquaculture. Journal of Physics: Conference Series 1025: 012058. DOI: 10.1088/1742-6596/1025/1/012058.
Álvarez-Martínez, F.J., E. Barrajón-Catalán, M. Herranz-López and V. Micol. 2021. Antibacterial plant compounds, extracts and essential oils: An updated review on their effects and putative mechanisms of action. Phytomedicine 90: 153626. DOI: 10.1016/j.phymed.2021.153626.
An, Q., J.N. Ren, X. Li, G. Fan, S.S. Qu, Y. Song, Y. Li and S.Y. Pan. 2021. Recent updates on bioactive properties of linalool. Food and Function 12(21): 10370–10389. DOI: 10.1039/D1FO02120F.
Ashokkumar, K., V. Sampathrajan, M. Murugan, M.K. Dhanya, S. Aiswarya and N. Mathews. 2020. Chemical composition of Ocimum gratissimum essential oil from the South Western Ghats, India. Journal of Current Opinion in Crop Science 1(1): 27–30.
Assane, I.M., G.M.R. Valladão and F. Pilarski. 2020. Chemical composition, cytotoxicity and antimicrobial activity of selected plant-derived essential oils against fish pathogens. Aquaculture Research 52(2): 793–809.
Bakar, P., I.A.A. Rafi, W.R.W. Ahmad and K.B. Chu. 2019. Antimicrobial activities of commercial essential oils against Vibrio parahaemolyticus from acute hepatopancreatic necrosis disease of white shrimp (P. vannamei). Malaysian Fisheries Journal 18: 102–115.
Cazella, L.N., J. Glamoclija, M. Soković, J.E. Gonçalves, G.A. Linde, N.B. Colauto and Z.C. Gazim. 2019. Antimicrobial activity of essential oil of Baccharis dracunculifolia DC (Asteraceae) aerial parts at flowering period. Frontiers in Plant Science 10: 27. DOI: 10.3389/fpls.2019.00027.
Chagas, E.C., C. Majolo, P.C. Monteiro, M.R.D. Oliveira, P.E. Gama, H.R. Bizzo and F.C.M. Chaves. 2020. Composition of essential oils of Mentha species and their antimicrobial activity against Aeromonas spp. Journal of Essential Oil Research 32(3): 209–215. DOI: 10.1080/10412905.2020.1741457.
Changchai, N. and S. Saunjit. 2014. Occurrence of Vibrio parahaemolyticus and Vibrio vulnificus in retail raw oysters from the eastern coast of Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health 45(3): 662–669.
Diniz do Nascimento, L., A.A.B.D. Moraes, K.S.D. Costa, J.M.P. Galúcio, P.S. Taube, C.M.L. Costa, J.N. Cruz, E.H.D.A. Andrade and L.J.G.D. Faria. 2020. Bioactive natural compounds and antioxidant activity of essential oils from spice plants: new findings and potential applications. Biomolecules 10(7): 988. DOI: 10.3390/biom10070988.
Dung, P.N.T., T.P. Dao, T.T. Le, H.T. Tran, T.T.T. Dinh, Q.L. Pham, Q.T. Tran and M.Q. Pham. 2021. Extraction and analysis of chemical composition of Ocimum gratissimum L essential oil in the North of Vietnam. IOP Conference Series: Materials Science and Engineering 1092(1): 012092. DOI: 10.1088/1757-899X/1092/1/012092.
El Atki, Y., I. Aouam, F.E. Kamari, A. Taroq, A. Gourch, B. Lyoussi and A. Abdellaoui. 2019. Antibacterial efficacy of thymol, carvacrol, eugenol and menthol as alternative agents to control the growth of nosocomial infection-bacteria. Journal of Pharmaceutical Sciences and Research 11(2): 306–309.
Ervia, Y., Subagiyo and A. Nuril. 2021. Antimicrobial susceptibility and minimum inhibition concentration of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio harveyi isolated from a white shrimp (Litopenaeus vannamei) pond. IOP Conference Series: Earth and Environmental Science 763: 012025. DOI: 10.1088/1755-1315/763/1/012025.
Fidan, H., G. Stefanova, I. Kostova, S. Stankov, S. Damyanova, A. Stoyanova and V.D. Zheljazkov. 2019. Chemical composition and antimicrobial activity of Laurus nobilis L. essential oils from Bulgaria. Molecules 24(4): 804. DOI: 10.3390/molecules24040804.
Filho, J.G.D.O., G.D.C. Silva, A.C.D. Aguiar, L. Cipriano, H.M.C.D. Azeredo, S.B. Junior and M.D. Ferreira. 2021. Chemical composition and antifungal activity of essential oils and their combinations against Botrytis cinerea in strawberries. Journal of Food Measurement and Characterization 15: 1815–1825. DOI: 10.1007/s11694-020-00765-x.
Gairola, K., S. Gururani, R. Kumar, O. Prakash, S. Agarwal and S.K. Dubey. 2021. Phytochemical composition, antioxidant, and anti-inflammatory activities of essential oil of Acmella uliginosa (Sw.) Cass. grown in North India Terai region of Uttarakhand. Trends in Phytochemical Research 5(1): 44–52. DOI: 10.30495/tpr.2021.680495.
Githaiga, B.M., E.M. Gathuru, P.N. Waithaka and L.W. Kiarie. 2018. Determination of antibacterial activity of essential oils from mint (Mentha spicata) leaves on selected pathogenic bacteria. Journal of Drugs and Pharmaceutical Science 2(2): 8–14. DOI: 10.31248/JDPS2018.015.
Haydari, M., V. Maresca, D. Rigano, A. Taleei, A.A. Shahnejat-Bushehri, J. Hadian, S. Sorbo, M. Guida, C. Manna, M. Piscopo, R. Notariale, F.D. Ruberto, L. Fusaro and A. Basile. 2019. Salicylic acid and melatonin alleviate the effects of heat stress on essential oil composition and antioxidant enzyme activity in Mentha×Piperita and Mentha Arvensis L. Antioxidants 8(11): 547. DOI: 10.3390/antiox8110547.
Hu, F., X.F. Tu, K. Thakur, F. Hu, X.L. Li, Y.S. Zhang, J.G. Zhang and Z.J. Wei. 2019. Comparison of antifungal activity of essential oils from different plants against three fungi. Food and Chemical Toxicology 134(3): 110821. DOI: 10.1016/j.fct.2019.110821.
Irkin, R., S. Carikci, S. Akalin and Z. Batu. 2021. Decontamination effect of lemon peel and mint leaf extracts on salad vegetables. Journal of Food and Nutrition Research 60(2): 146–152.
Jha, R.K., Y.H. Babikian, H.Y. Babikian, L.V. Khoa, D. Wisoyo, S. Srisombat and B. Jiaravanon. 2016. Efficacy of natural herbal formulation against acute hepatopancreatic necrosis disease (AHPND) causing Vibrio parahaemolyticus in Penaeus vannamei. Veterinary Medicine 2(1): 1–6. DOI: 10.17140/VMOJ-2-109.
Kumar, A., P. Singh, V. Gupta and B. Prakash. 2020. 8-Application of nanotechnology to boost the functional and preservative properties of essential oils. In: Functional and Preservative Properties of Phytochemicals (ed. B. Prakash), pp. 241–267. Academic Press, Cambridge, Massachusetts, USA. DOI: 10.1016/B978-0-12-818593-3.00008-7.
Li, Z.H., M. Cai, Y.S. Liu, P.L. Sun and S.L. Luo. 2019. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules 24(8): 1577. DOI: 10.3390/molecules24081577.
Lu, W.C., D.W. Huang, C.C.R. Wang, C.H. Yeh, J.C. Tsai, Y.T. Huang and P.H. Li. 2018. Preparation, characterization, and antimicrobial activity of nanoemulsions incorporating citral essential oil. Journal of Food and Drug Analysis 26(1): 82–89. DOI: 10.1016/j.jfda.2016.12.018.
Lykov, A., L. Rachkovskaya, E. Rachkovsky and O. Poveshchenko. 2022. Effect of the composition of aluminum-silica carrier with menthol, camphor, acidum boricum, and colloidal silver on monocytes lines function. Proceedings of Ural-Siberian Conference on Computational Technologies in Cognitive Science, Genomics and Biomedicine (CSGB) 2022: 22009851. DOI: 10.1109/CSGB56354.2022.9865261.
Majewska, E., M. Kozłowska, E. Gruczyńska-Sękowska, D. Kowalska and K. Tarnowska. 2019. Lemongrass (Cymbopogon citratus) essential oil: extraction, composition, bioactivity and uses for food preservation–a review. Polish Journal of Food and Nutrition Sciences 69(4): 327–341. DOI: 10.31883/pjfns/113152.
Majolo, C., P.C. Monteiro, A.V.P.D. Nascimento, F.C.M. Chaves, P.E. Gama, H.R. Bizzo and E.C. Chagas. 2019. Essential oils from five Brazilian piper species as antimicrobials against strains of Aeromonas hydrophila. Journal of Essential Oil Bearing Plants 22(3): 746–761. DOI: 10.1080/0972060X.2019.1645047.
Man, A., L. Santacroce, R. Jacob, A. Mare and L. Man. 2019. Antimicrobial activity of six essential oils against a group of human pathogens: A comparative study. Pathogens 8(1): 15. DOI: 10.3390/pathogens8010015.
Manh, H.D. and O.T. Tuyet. 2020. Larvicidal and repellent activity of Mentha arvensis L. essential oil against Aedes aegypti. Insects 11(3): 198. DOI: 10.3390/insects11030198.
Martínez-Pabón, M.C. and M. Ortega-Cuadros. 2020. Thymol, menthol and eucalyptol as agents for microbiological control in the oral cavity: A scoping review. Revista Colombiana de Ciencias Químico-Farmacéuticas 49(1): 44–69. DOI: 10.15446/rcciquifa.v49n1.87006.
Melo, R.S., Á.M.A. Azevedo, A.M.G. Pereira, R.R. Rocha, R.M.B. Cavalcante, M.N.C. Matos, P.H.R. Lopes, G.A. Gomes, T.H.S. Rodrigues, H.S.D. Santos, I.L. Ponte, R.A. Costa, G.S. Brito, F.E.A.C. Júnior and V.A. Carneiro. 2019. Chemical composition and antimicrobial effectiveness of Ocimum gratissimum L. essential oil against multidrug-resistant isolates of Staphylococcus aureus and Escherichia coli. Molecules 24(21): 3864. DOI: 10.3390/molecules24213864.
Mittal, R., R. Kumar and H.S. Chahal. 2018. Antimicrobial activity of Ocimum sanctum leaves extracts and oil. Journal of Drug Delivery and Therapeutics 8(6): 201–204. DOI: 10.22270/jddt.v8i6.2166.
Monteiro, P.C., C. Majolo, F.C.M. Chaves, H.R. Bizzo, F.L.A. O’Sullivan and E.C. Chagas. 2020. Antimicrobial activity of essential oils from Lippia sidoides, Ocimum gratissimum and Zingiber officinale against Aeromonas spp. Journal of Essential Oil Research 33(2): 152–161. DOI: 10.1080/10412905.2020.1848653.
Muralidharan, V. and M. Deecaraman. 2019. In vitro susceptibility of commonly used antibiotics against Vibrio parahaemolyticus, isolated from finfishes, Chennai, India. Journal of Infectious Diseases and Treatment 5(1): 1-4.
Nabila, B., A. Piras, B. Fouzia, D. Falconieri, G. Kheira, F.F. Fedoul and S.R. Majda. 2022. Chemical composition and antibacterial activity of the essential oil of Laurus nobilis leaves. Natural Product Research 36(4): 989–993. DOI: 10.1080/14786419.2020.1839450.
Nabti, L.Z., F. Sahli, H. Laouer, A. Olowo-Okere, J.G.N. Wandjou and F. Maggi. 2020. Chemical composition and antibacterial activity of essential oils from the algerian endemic Origanum glandulosum Desf. against multidrug-resistant uropathogenic E. coli isolates. Antibiotics 9(1): 29. DOI: 10.3390/antibiotics9010029.
Ogunwande, I.A., O.N. Avoseh, K.N. Olasunkanmi, O.A. Lawal, R. Ascrizzi and G. Flamini. 2019. Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra. Journal of Ethnopharmacology 232: 188–192. DOI: 10.1016/j.jep.2018.12.017.
Oro, V., S. Krnjajic, M. Tabakovic, J. Stanojević and S. Ilic-Stojanovic. 2020. Nematicidal activity of essential oils on a psychrophilic Panagrolaimus sp. (Nematoda: Panagrolaimidae). Plants 9(11): 1588. DOI: 10.3390/plants9111588.
Pateiro, M., F.J. Barba, R. Domínguez, A.S. Sant'Ana, A.M. Khaneghah, M. Gavahian, B. Gómez and J.M. Lorenzo. 2018. Essential oils as natural additives to prevent oxidation reactions in meat and meat products: A review. Food Research International 113: 156–166. DOI: 10.1016/j.foodres.2018.07.014.
Peichel, C., D.V.T. Nair, G. Dewi, A.M. Donoghue, K.M. Reed and A.K. Johny. 2019. Effect of Lemongrass (Cymbopogon citratus) Essential oil on the survival of multidrug-resistant Salmonella enterica serovar Heidelberg in contaminated poultry drinking water. Journal of Applied Poultry Research 28(4): 1121–1130. DOI: 10.3382/japr/pfz076.
Piras, A., S. Porcedda, D. Falconieri, A. Maxia, M. Gonçalves, C. Cavaleiro and L. Salgueiro. 2019. Antifungal activity of essential oil from Mentha spicata L. and Mentha pulegium L. growing wild in Sardinia island (Italy). Natural Product Research 35(6): 993–999. DOI: 10.1080/14786419.2019.1610755.
Sanli, A. and T. Karadoğan. 2017. Geographical impact on essential oil composition of endemic Kundmannia anatolica Hub.-Mor. (Apiaceae). African Journal of Traditional, Complementary and Alternative Medicines 14(1): 131–137.
Sevik, R., G. Akarca, M. Kilinc and Ç. Ascioglu. 2021. Chemical composition of tea tree (Melaleuca alternifolia) (Maiden & Betche) Cheel essential oil and its antifungal effect on foodborne molds isolated from meat products. Journal of Essential Oil Bearing Plants 24(3): 561–570. DOI: 10.1080/0972060X.2021.1942232.
Shanaida, M., N. Hudz, M. Białoń, M. Kryvtsowa, L. Svydenko, A. Filipska and P.P. Wieczorek. 2021. Chromatographic profiles and antimicrobial activity of the essential oils obtained from some species and cultivars of the Mentheae tribe (Lamiaceae). Saudi Journal of Biological Sciences 28(11): 6145–6152. DOI: 10.1016/j.sjbs.2021.06.068.
Sharma, S., S. Habib, D. Sahu and J. Gupta. 2021. Chemical properties and therapeutic potential of citral, a monoterpene isolated from lemongrass. Medicinal Chemistry 17(1): 2-12. DOI: 10.2174/1573406416666191227111106.
Shendurse, A.M., R.B. Sangwan, A. Kumar, R. Venkateshappa, A.C. Patel, G. Gopikrishna and S.K. Roy. 2021. Phytochemical screening and antibacterial activity of lemongrass (Cymbopogon citratus) leaves essential oil. Journal of Pharmacognosy and Phytochemistry 10(2): 445–449.
Soltanbeigi, A., M. Özgüven and M.B. Hassanpouraghdam. 2021. Planting-date and cutting-time affect the growth and essential oil composition of Mentha×piperita and Mentha arvensis. Industrial Crops and Products 170(7): 113790. DOI: 10.1016/j.indcrop.2021.113790.
Souza, C.F., M.D. Baldissera, B. Baldisserotto, B.M. Heinzmann, J.A. Martos-Sitcha and J.M. Mancera. 2019. Essential oils as stress-reducing agents for fish aquaculture: A review. Front in Physiol 10: 785. DOI: 10.3389/fphys.2019.00785.
Sun, H., Y. Li, X. Shi, Y. Lin, Y. Qiu, J. Zhang, Y. Liu, M. Jiang, Z. Zhang, Q. Chen, Q. Sun and Q. Hu. 2014. Association of CRISPR/Cas evolution with Vibrio parahaemolyticus virulence factors and genotypes. Foodborne Pathogens and Disease 12(1): 68-73. DOI: 10.1089/fpd.2014.1792.
Tamer, C.E., S. Suna and G. Özcan-Sinir. 2019. 14-Toxicological aspects of ingredients used in nonalcoholic beverages. In: Non-Alcoholic Beverages (eds. A. Mihai Grumezescu and A.M. Holban), pp. 441–481. Woodhead Publishing, Cambridge, UK.
Ugbogu, O.C., E. Okezie, G.O. Agi, C. Ibe, C.N. Ekweogu, V.C. Ude, M.E. Uche, R.O. Nnanna and E.A. Ugbogu. 2021. A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.). Heliyon 7(11): e08404. DOI: 10.1016/j.heliyon.2021.e08404.
Vu, T.X., T.B. Tran, C.Q. Hoang, H.T. Nguyen, M.X.B. Phan, A.N. Dao, M.T. Dinh, K. Soytong and H.Q. Nguyen. 2021. Chemical compositions and anti-malassezia properties of Vietnamese Mentha arvensis and Piper betle essential oils. International Journal of Agricultural Technology 17(4): 1619–1630.
World Organisation for Animal Health (OIE). 2019. Manual of diagnostic test for aquatic animals. Chapter 2.2.1: Acute hepatopancreatic necrosis disease. https://www.woah.org/en/what-we-do/standards/codes-and-manuals/aquatic-manual-online-access/. Cited 1 Oct 2022.
Yadav, M., R. Dhar, S. Singh and T. Allen. 2019. Comparative study of antimicrobial activity of lemongrass (Cymbopogon citratus), clove (Syzygium aromaticum), and tulsi (Ocimum) essential oils against foodborne pathogens. Asian Journal of Pharmaceutical and Clinical Research 12(4): 230–234. DOI: 10.22159/ajpcr.2019.v12i4.29451.
Yuan, W., C.H.M. Teo and H.G. Yuk. 2018. Combined antibacterial activities of essential oil compounds against Escherichia coli O157:H7 and their application potential on fresh-cut lettuce. Food Control 96: 112–118. DOI: 10.1016/j.foodcont.2018.09.005.
Zhang, Y., J. Wei, H. Chen, Z. Song, H. Guo, Y. Yuan and T. Yue. 2020. Antibacterial activity of essential oils against Stenotrophomonas maltophilia and the effect of citral on cell membrane. LWT–Food Science and Technology 117(7): 108667. DOI: 10.1016/j.lwt.2019.108667.
Zheng, X., A.F. Feyaerts, P.V. Dijck and P. Bossier. 2020. Inhibitory activity of essential oils against Vibrio campbellii and Vibrio parahaemolyticus. Microorganisms 8(12): 1946. DOI: 10.3390/microorganisms8121946.