The Potential of Donkey’s Ear Abalone (Haliotis asinina Linnaeus, 1758) as an Antibacterial Agent and Its Effect on Blood Cholesterol Reduction in Mice (Mus musculus Linnaeus, 1758)
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Abstract
Donkey's ear abalone (Haliotis asinina Linnaeus, 1758) is rich in essential nutrients and harbors bioactive compounds with antibacterial properties, potentially aiding in cholesterol reduction. This study focused on assessing antibacterial activity against Staphylococcus aureus (ATCC25923) and Escherichia coli (ATCC25922), determining the amino acid composition, screening for bioactive phytochemical compounds, and evaluating the effect of abalone meat extract on blood cholesterol in male house mice (Mus musculus Linnaeus, 1758) using an in vivo method. Results indicated significant antibacterial activity of H. asinina methanol extract (20% concentration) against S. aureus, while ethyl acetate extract (10% and 20% concentrations) showed inhibition zones against both S. aureus and E. coli. The n-hexane extract demonstrated antibacterial activity at a 10% concentration on both organisms, and at a 20% concentration against S. aureus. Phytochemical screening revealed the presence of saponins and alkaloids in the methanol extract. Amino acid analysis identified arginine (7,827.15 mg·kg-1) as the highest essential amino acid and glutamic acid (14,803.9 mg·kg-1) as the highest non-essential amino acid. Moreover, the methanol extract significantly reduced cholesterol levels in male house mice across various doses (100, 200, and 300 mg·kg-1).
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