Moisturizing Efficacy of Stichopus hermanii-Derived Collagen in Balb/c Mice (Mus musculus)
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Abstract
The gamma sea cucumber (Stichopus hermanii) is a marine invertebrate known for its high proteins and essential fatty acids, which may enhance collagen production and fibroblast proliferation, making it a valuable resource for health, particularly for skin and tissue regeneration and cosmetic applications. This study investigates the potential of a sea cucumber collagen extract formulated in a moisturizing cream to increase collagen levels and improve skin condition in Mus musculus. Collagen was extracted through sequential maceration with NaOH, CH2, COOH, and distilled water. The cream was tested for organoleptic properties, pH, washability, and homogeneity. Four groups of animals were treated with 10% sea cucumber collagen cream (P1), commercial cream (P2), base cream (vehicle control), and no treatment (untreated control) for 5 weeks. Collagen yield was 1.25% (w/w), and presented as a yellow, odorless solid. The P1 cream exhibited white color with brown granules, a waxy odor, and a semi-solid texture. Testing showed that P1 resulted in an 86.66±5.16% collagen increase, significantly higher than P2 (75.00±5.77%) and the vehicle control (73.33%±4.71%, p<0.05), without significantly differing from the untreated control (85.00±5.00 %).
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