Interactive Influence of Stocking Density and Dietary L-Tryptophan on Growth Performance, Physiological Responses and Cannibalism in Striped Snakehead (Channa striata) (Bloch, 1793)
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Abstract
A -day experiment was conducted to investigate the interactive effects of stocking density and dietary L-tryptophan on the growth performance and physiological responses of striped snakehead (Channa striata) during the nursery rearing period. Advanced fry (0.32±0.293 were randomly stocked in 36 glass aquaria in a 3×4 factorial design, with three stocking densities (375, 500, and 625 fish·m⁻³) and four dietary L-tryptophan levels (0%, 1%, 2%, 3%). Each treatment was triplicated. Results ed that fry reared at 375 fish⸳m⁻³ and fed 3% L-tryptophan had significantly higher weight gain (4.67±0.04 g), specific growth rate (4.68±0.09%·day⁻¹), and feed efficiency ratio (1.33±0.02), as well as lower feed conversion ratio (0.75±0.02) and cannibalism (26.7±0.30%) than other groups. This treatment also produced higher RBC (4.07±0.05), Hb (9.76±0.25), Ht (35.1±0.32) and greater digestive enzyme activities, including protease, lipase, and amylase. Higher espiratory burst activity (0.41±0.01) was recorded at 375 fish⸳m⁻³ with 0% L-tryptophan, while highest antioxidant enzyme activities, catalase and superoxide dismutase, were observed at 625 fish⸳m⁻³ with 3% L-tryptophan. Survival rate (69.93±1.11%) was observed at 375 fish⸳m⁻³ with 3% L-tryptophan. No abnormalities were observed in the intestine or liver of L-tryptophan fed groups, whereas severe hepatocyte vacuolations were observed in control. Therefore, 375 fish⸳m⁻³ with 3% L-tryptophan is the ideal combination for improving growth, health performance and survival while reducing stress and cannibalism.
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