Effect of Salinity on Previtellogenic Development of Female Pacific White Shrimp (Litopenaeus vannamei Boone, 1931)

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Keywords:
Salinity previtellogenic stage reproductive hormones and genes ovarian development

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Ekachai Duangjai
Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Thailand
Wanvipa Suthikrai
Faculty of Veterinary Science, Chulalongkom University, Thailand
Narongsak Puanglarp
Department of Marine Science, Faculty of Science, Chulalongkorn University, Thailand
Prapansak Srisapoome
Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Thailand
Panida Unagul
National Center for Genetic Engineering and Biotechnology (BIOTEC), Genome Institute, Thailand
Orapom Meunpol
Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Thailand

Abstract

Successful induced maturation of female shrimp consists of three stages of gonadal development, namely, previtellogensis, vitellogensis, and final maturation. Previous research in shrimp reproduction investigated mostly the final maturation stage and spawning process, and few studies were conducted on previtellogenic ovarian development. Several factors influence previtellogenesis of female Pacific white shrimp (Litopenaeus vannamei Boone, 1931). In this study, the effect of salinity, one of the most powerful factors, was investigated. Female L. vannamei, (45 g body weight, 120 days old) with previtellogenic ovaries were reared in cages (5x5 m2) installed in two earthen ponds. Salinity levels were 15 and 30 ppt. Haemolymph, hepatopancreas and ovaries of the experimental shrimp were collected monthly for hormone analysis. P4, E2 and PGF2α levels in haemolymph of the shrimp samples were measured bp either RP-HPLC or RIA. Biological marker (Primer) specific for 357 bp of Vg and 314 by of FAMeT gene modified from NCBI were developed from the hepatopancreas of female L. vannamei. Vg and FAMeT gene expression levels using 1stcDNA synthesis from hepatopancreas and ovary tissues were determined by RT-PCR. Results showed that the levels of Vg and FAMeT gene expression in the ovaries and hepatopancreas of shrimp reared in 30 ppt were significantly higher than those of shrimp reared in 15 ppt (P<0.05). The mean expression level of Vg and FAMeT in shrimp hepatopancreas was higher than in the ovaries. Similarly, changes in P4, E2 and PGF2α concentrations in haemolymph and GSI of shrimp reared in 30 ppt were significantly higher than those of shrimp reared in 15 ppt (P<0.05). In conclusion, the experiment showed that salinity played an important role in shrimp ovarian development at different levels of changes, namely morphological (GSI), hormonal and gene expression levels.

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Duangjai, E., Suthikrai, W., Puanglarp, N., Srisapoome, P., Unagul, P., & Meunpol, O. (2015). Effect of Salinity on Previtellogenic Development of Female Pacific White Shrimp (Litopenaeus vannamei Boone, 1931). Journal of Fisheries and Environment, 39(3), 15–29. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/80482
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Vol. 39 No. 3 (2015): September-December
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Articles

References

1. Arcos, F. G., Ibarra, A. M. and L.S. Racotta. 2011. Vitellogenin in hemolymph predicts gonad maturity in adult female Litopenaeus (Penaeus) vannamei shrimp. Aquaculture. 316: 93-98.

2. Boone, L. 1931. A collection of anomuran and macruran crustacea from the bay of Panama and freshwater of the Canal Zone. Bulletin of the American Museum of Natural History. 63: 137-189.

3. Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 28:350-356.

4. Chen, S.H., Lin C.Y. and C.M. Kuo. 2004. Cloning of two crustacean hyperglycemic hormone isoforms in freshwater giant prawn Macrobrachium rosenbergii: evidence of alternative splicing. Mar Biotech. 6: 83-94.

5. Coman, G. J., Arnold, S. J., Jones, M. J. and N. P. Preston. 2007. Effect of rearing density on growth, survival and reproductive performance of domesticated Penaeus monodon. Aquaculture. 264: 175-183.

6. D’Croz, L, Wong, L.V, Justine, G. and M. Gupta. 1988. Prostaglandins and related compounds from the polychaete worm Americonuphis reesi Fauchald (Onuphidae) as possible inducers of gonad development in penaeid shrimps. Rev Biol Trop. 36:331–332.

7. Fingerman, M. 1995. Endocrine mechanisms in crayfish, with emphasis on reproduction and neurotransmitter regulation of hormone release. Am. Zool. 35: 68-78.

8. Fingerman, M. 1997. Roles of neurotransmitters in regulating reproductive hormones release and gonad maturation in decapod crustaceans. Invertebr. Reprod. Dev. 31:47-54.

9. Ghazy, M. M., Habashy, M. M. Kossa, F.I. and E. Y. Mohammady. 2009. Effects of Salinity on Survival, Growth and Reproduction of the Water Flea, Daphnia magna. Nature & Science. 7: 28-42.

10. Hiransuchalert, R., Thamniemdee, N., Khamnamtong, B., Yamano, K. and S. Klinbunga. 2013. Expression profiles and localization of vitellogenin mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon. Aquaculture. 412–413: 193-201.

11. Hoa, N. D., Wouters, R., Wille, M., Thanh, V., Dong, T. K., Van Hao, N. and P. Sorgeloos. 2009. A fresh-food maturation diet with an adequate HUFA composition for broodstock nutrition studies in black tiger shrimp Penaeus monodon. Aquaculture. 297: 116-121.

12. Kamonpatana, M., D. F.M. Van De Wie, W. Koops, L. Dumtong, Ngramsuriyaroj, C. and S. Usanakornkul. 1979. Oestrus control and early pregnancy diagnosis in swamp buffalo: comparison of enzyme immunoassay and radio immunoassay for plasma progesterone. Theriogenology. 11(5): 399-409.

13. Limsuwan, C. and P. Chanratchakool. 2004. Shrimp Industry of Thailand. Magic Publication Co, Ltd. Bangkok. 206 pp.

14. Nguyen, M. T., Barnes, A. C., Mather, P. B., Li, Y. T. and R. E. Lyons. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture. 301: 7-15.

15. Meunpol, O., Meejing, P. and S. Piyatiratitivorakul. 2005. Maturation diet based on fatty acid content for male Penaeus monodon (Fabricius) broodstock. Aquaculture Research. 36: 1216-1225.

16. Meunpol, O., Iam-Pai, S., Suthikrai, W. and S. Piyatiratitivorakul. 2007. Identification of progesterone and 17a-hydroxyprogesterone in polychaetes (Perinereis sp.) and the effects of hormone extracts on penaeid oocyte development in vitro. Aquaculture. 270: 485–492.

17. Meunpol, O., Duangjai, E., Yoonpun, R. and S. Piyatiratitivorakul. 2010. Detection of prostaglandin E2 in polychaete Perinereis sp. and its effect on Penaeus monodon oocyte development in vitro. Fisheries Science. 76: 281-286.

18. Morera, Y., Lugo, J. M., Ramos, L., Rodriguez-Ramos, T., Huberman, A. and M. P. Estrada. 2013. A chimeric recombinant crustacean hyperglycemic hormone from Litopenaeus schmitti (Burkenroad) obtained as C-terminus fusion protein boost hemolymph glucose concentration in Litopenaeus vannamei. Aquaculture Research. 44: 1066-1075.

19. Okumura, T., and K. Sakiyama. 2004. Hemolymph levels of vertebrate-type steroid hormones in female kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda: Penaeidae) during natural reproductive cycle and induced ovarian development by eyestalk ablation. Fisheries Science. 70: 372-380.

20. Palacios, E., Carreño, D., Rodrígez-Jaramillo, M.C. and I.S. Racotta. 1999. Effect of eyestalk ablation on maturation, larval performance, and biochemistry of Penaeus vannamei broodstock. J. Appl. Aquac. 9: 1–23.

21. Reddy, P.S., Reddy, P.R.and G.P.C. Nagaraju. 2004. The synthesis and effects of prostaglandins on the ovary of the crab Oziotelphusa senex senex. Gen Comp Endocrinol. 135: 35–4.

22. Sainz-Hernandez, J. C., Racotta, I. S., Dumas, S. andJ. Hernandez-Lopez. 2008. Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables. Aquaculture. 283: 188-193.

23. Shinji, J., Kang, B., Okutsu, T., Banzai, K., Ohira, T., Tsutsui, N. and M. Wilder. 2012. Changes in crustacean hyperglycemic hormones in Pacific whiteleg shrimp Litopenaeus vannamei subjected to air-exposure and low-salinity stresses. Fisheries Science. 78: 833-840.

24. Silva Gunawardene, Y.I.N., Chow, B.K.C., He, J.G. and S.M. Chan. 2001. The shrimp FAMeTcDNA is encoded for a putative enzyme involved in the methylfarnesoate (MF) biosynthetic pathway and is temporally expressed in the eyestalk of different sexes. Insect Biochem. Mol. Biol. 31: 1115–1124.

25. Tahara, D.and I.Yano. 2003. Development of hemolymph prostaglandins assay systems and their concentration variations during ovarian development in the kuruma shrimp, Penaeus japonicus. Aquaculture. 220: 791–800.

26. Tahara, D. and I. Yano. 2004. Development-related variations in prostaglandin and fatty acid content of ovary in the kuruma shrimp (Marsupenaeus japonicus). Comp Biochem Phys. 137:631–637.

27. Tsukimura, B. and F.I. Kamemoto. 1991. In vitro stimulation of oocytes by presumptive mandibular secretions in the shrimp Penaeus vannamei. Aquaculture. 92: 59–66.

28. Treerattrakool, S., Panyim, S. and A. Udomkit. 2011. Induction of ovarian maturation and spawning in Penaeus monodon broodstock by double-stranded RNA. Mar Biotechn. 13: 163-169.