Effect of Dietary Polyphenol-Rich Feed Additive from Grape Pomace on Growth, Survival and Tolerance to Vibrio Infection in Pacific White Shrimp (Litopenaeus vannamei)
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Abstract
The effect of Anta®Ox FlavoSyn, polyphenol-rich feed additive from grapes, on the health and tolerance to Vibrio infection in Pacific white shrimp was studied under laboratory conditions. In experiment 1, postlarvae 12 were stocked in an 18 x 500-L fiberglass tank at a density of 50 PL/tank. Water temperature and salinity were maintained at 29 ± 1°C and 20–25 ppt, respectively. Shrimp were distributed into three groups (with six replicates/treatment), then fed four times/day with normal pelleted feed (control group), or feed mixed with 400 and 800 ppm Anta®Ox FlavoSyn. After 60 days of feeding trials, the body weights of shrimp fed with 400 and 800 ppm Anta®Ox FlavoSyn were 3.42 ± 0.22 and 3.48 ± 0.18 g, respectively, both significantly higher than those of the control group (2.64 ± 0.43 g). However, there was no difference in the survival rates and FCR from the three experimental groups. In experiment 2, 30 shrimp from experiment 1 were randomly stocked in new 18 x 500-L fiberglass tanks with six replicate tanks per treatment. At the beginning of the trial and 14 days later, Vibrio parahaemolyticus was added into each tank to obtain a final concentration of 104 CFU/ml. Each treatment group was fed the aforementioned diets four times daily for 30 days. All water quality parameters were maintained as in experiment 1. At the end of this experiment, 800 ppm Anta®Ox FlavoSyn-fed shrimp had a survival rate of 78.33 ± 1.92%, significantly higher than that of the control group (64.17 ± 1.67%). The hepatopancreas of the former showed less necrotic cells as well. Nevertheless, weight gain, total intestinal Vibrio spp., and total bacterial count were not significantly different among all experimental groups. The present study indicated that Anta®Ox FlavoSyn had a positive effect on their growth and survival.
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References
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