Changes in Serum Concentration of Dopamine, 17 -Estradiol Progesterone During Reproductive Cycle of Gunther's Walking Catfish (Clarias macrocephalus)
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Abstract
Seed production of walking catfish (Clarias macrocephalus) in Thailand has been declining due to the poor reproductive performance of the female walking catfish. One possible problem might come from the failure of reproductive hormones responsible for ovarian maturation of broodstock. Therefore, this study aimed to investigate the normal patterns of related reproductive hormones coupling with ovarian development of female walking catfish for one full spawning cycle. Blood of female catfish broodstock was collected for hormone analysis by HPLC and RIA. Results showed that at the start of the reproductive cycle (February to April), high DA levels (114.54±28.57 Jlg/ml) were concomitant with P4 levels (1.84± .36 ng/ml). P4 gradually decreased when entering the spawning period (June to August) until it hits its lowest at 0.78 ± 0.26 ng/ml in August (p<0.05), which gave way to an increase of E2 levels (0.68±0.42 ng/ml). After the spawning season (October to December), DA and E2 levels in the fish blood declined slightly, while P4 increased gradually for the next breeding season. The results also showed that the DA and P4 in female walking catfish were important at the initiation of the pre-spawning season whereas E2 was crucial for vitellogenesis
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2. Aizen, J., Meiri, I., Tzchori, I., Levavi-Sivan, B. and H. Rosenfeld. 2005. Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition. Gen.Comp. Endocrinol. 142: 212-221.
3. Atteke, C., Vetillard, A., Postier, A., Gamier, D-H., Jego, P., and T. Bailhachea. 2003. Effects of progesterone and estradiol on the reproductive axis in immature diploid and triploid rainbow trout. Comparative Biochemistry and Physiology Part A. 34: 693-705.
4. Dufour S., Sebert, M.-E., Weltzien, F.-A., Rousseau, K., and C. Pasqualini. 2010. Neuroendocrine control by dopamine of teleost reproduction. Journal of Fish Biology.76:129-160.
5. Ingthamjitr, S., 1997. Hybrid Clarias catfish seed production and marketing in central Thailand. PhD thesis. Asian Institute of Technology, Bangkok, Thailand
6. Kamonpatana, M.,Wie, D.F.M., Koops, V. D.W, Dmntong, L., Ngramsuriyaroj C., and S. Usanakornkul. 1979. Oestrus control and early pregnancy diagnosis in swamp buffalo: comparison of enzyme immunoassay and radio immunoassay and radio immunoassay for plasma progesterone. Theriogenology. 11(5): 399-409.
7. Lee, W.-K and S.-W Yang. 2002. Relationship between ovarian development and serum levels of gonadal steroid hormones, and induction of oocyte maturation and ovulation in the cultured female Korean spotted sea bass Lateolabrax maculatus (Jeom nong-eo). Aquaculture. 207:169- 183.
8. Meunpol, O. 1988. Effects of hormones on final oocyte maturation of Pia Duk Dan (Clarias batrachus Linn.). Thesis. Kasetsart University, Bangkok, Thailand.
9. Muzzi, C., Bertocci, E., Terzuoli, L., Porcelli, B., Ciari, I., Pagani, R., and R. Guerranti. 2008. Simultaneous determination of serum concentrations of levodopa, dopamine, 3 - 0 - methyldopa and a - methyldopa by HPLC. Biomedicine & Pharmacotherapy. 62:253-258.
10. Pham, H. Q., Kjersvik, E., Nguyen, A.T., Nguyen, M. D., and A. Arukwe. 2010. Reproductive cycle in female Waigieu seaperch (Psammoperca waigiensis) reared under different salinity levels and the effects of dopamine antagonist on steroid hormone levels. Journal of Experimental Marine Biology and Ecology. 383:137-145.
11. Vacher, C. Ferriere, F. Marmignon, M-H. Pellegrini, E.and C. Saligaut. 2002. Dopamine D2 receptors and secretion of FSH and LH: role of sexual steroids on the pituitary of the female rainbow trout. General and Comparative Endocrinology. 127:198-206.