Efficient Clarification of the Hepatopancreatic Homogenate of Red Shrimp During the Recovery of Crude Alkaline Phosphatase
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Keywords:
Alkaline phosphatase Red shrimp Insoluble Centrifugation Clarification Hepatopancreas
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Abstract
Centrifugal force and time for the primary clarification of hepatopancreatic homogenate of red shrimp was determined on the optimum recovery of alkaline phospatase. Centrifugal force of 1681.1×g for 5 min or more than 1681.1×g for 5, 10, 15, or 20 min successfully clarify 96.99±0.45% of the total solids, 93.16±1.03% of soluble protein, and 93.61±0.16% of the lipids from the respective initial homogenate. However, the centrifugal force more than 1681.1×g caused denaturation of alkaline phosphatase due to hydrodynamic sheer even at the temperature of 4oC. Centrifugal force of 1681.1×g for 5 min was able to retain 94.29±1.44% of the alkaline phosphatase of the initial homogenate, eventhough clarified 89.98±0.01% of the total solids, 77.25±0.89% of soluble protein, and 78.76±0.64% of the lipids from the respective initial homogenate. Centrifugal force of 1681.1×g for 5 min is an efficiently removes insoluble while retaining optimum alkaline phosphatase activity.
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Nooralabettu, K. P. (2014). Efficient Clarification of the Hepatopancreatic Homogenate of Red Shrimp During the Recovery of Crude Alkaline Phosphatase. Journal of Fisheries and Environment, 38(1), 8–19. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/80602
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References
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15. Shenker, O., Tietz, A., Ovadia, M., Tom, M. 1993. Lipid synthesis from acetate by the in vitro incubated ovaries of the penaeid shrimp Penaeus semisulcatus. Marine Biology 117 (4): 583-589.
16. Shringari, S.M., Pakalapati, S.R., Singh, AB. 2012. Modelling the Rheological Behaviour of Enzyme Clarified Lime (Citrus aurantifolia L.) Juice Concentrate, Czech Journal of Food Science 30(5): 456–466.
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19. Yassien, M.A.M., Asfour, H.Z. 2012. Improved production, purification and some properties of α-amylase from Streptomyces clavifer. African Journal of Biotechnology 11(80): 14603-14611.
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2. Barnes, H., Blackstock, J. 1973. Estimation of Lipids in Marine Animals and Tissues: Detailed Investigation of the Sulphovanillin Method for 'Total' Lipids. Journal of Experimental Marine Biology and Ecology 12(1): 103-118.
3. Bowers, Jr. G.N., McComb, R.B. 1975. Measurement of total alkaline phosphatase activity in human serum. Clinical Chemistry 21(13): 1988-1995.
4. Chen, X., Xu, F., Qin, W., Ma, L., Zheng, Y. 2012. Optimization of enzymatic clarification of green asparagus juice using response surface methodology. Journal of Food Science 77(6): C665-670.
5. Elias, C.B, Joshi, J.B. 1998. Role of hydrodynamic shear on activity and structure of proteins. Advanced Biochemical Engineering 59(1): 47-71.
6. Erasmus, J.H., Cook, P.A., Coyne, V.E. 1997. The role of bacteria in the digestion of seaweed by the abalone Haliotis midae. Aquaculture 155(1-4): 377-386.
7. FAO. 1994. Support to regional aquaculture activities in Latin America and the Caribbean. In: Nutrition of Fish and Crustaceans-a Laboratory Manual. Olvera-Novoa MA, Martinez Palacios CA, Real de Leon E (eds). Food and Agriculture Organization of the United Nations, FAO, Mexico City, AB479/E: 61-62.
8. Hutchison, N., Bingham, N., Murrell, N., Farid, S., Hoare, M. 2006. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnology and Bioengineering 95(3): 483-491.
9. Iammarino, M., Nti-Gyabaah, J., Chandler, M., Roush, D., Göklen, K. 2007. Impact of Cell Density and Viability on Primary Clarification of Mammalian Cell Broth: An Analysis Using Disc-Stack Centrifugation and Charged Depth Filtration. BioProcess International 5(10): 38-50.
10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193(1): 265-275.
11. Maybury, J., Hoare, M., Dunnill, P. 2000. The use of laboratory centrifugation studies to predict performance of industrial machines: Studies of shear-insensitive and shear-sensitive materials. Biotechnology and Bioengineering 67(3): 265-273.
12. Nagahashi, J., Hiraike, K. 1982. Effects of Centrifugal Force and Centrifugation Time on the Sedimentation of Plant Organelles. Plant Physiology 69(1): 546-548.
13. Racek, A.A. 1955. Littoral penaeinae from New South Wales and adjacent Queensland waters. Australian Journal of Marine and Freshwater Research 6(2): 209-241.
14. Roush, D.J., Lu, Y. 2008, Advances in Primary Recovery: Centrifugation and Membrane Technology. Biotechnology Progress 24(1): 488-495.
15. Shenker, O., Tietz, A., Ovadia, M., Tom, M. 1993. Lipid synthesis from acetate by the in vitro incubated ovaries of the penaeid shrimp Penaeus semisulcatus. Marine Biology 117 (4): 583-589.
16. Shringari, S.M., Pakalapati, S.R., Singh, AB. 2012. Modelling the Rheological Behaviour of Enzyme Clarified Lime (Citrus aurantifolia L.) Juice Concentrate, Czech Journal of Food Science 30(5): 456–466.
17. Takagi, M., Ilias, M., Yoshida, T. 2000. Selective retention of active cells employing low centrifugal force at the medium change during suspension culture of Chinese hamster ovary cells producing tPA. Journal of Bioscience and Bioengineering 89(4): 340-344.
18. Wang, A., Lewus, R., Rathore, A.S. 2006. Comparison of different options for harvest of a therapeutic protein product from high cell density yeast fermentation broth. Biotechnology and Bioengineering 94(1): 91-104.
19. Yassien, M.A.M., Asfour, H.Z. 2012. Improved production, purification and some properties of α-amylase from Streptomyces clavifer. African Journal of Biotechnology 11(80): 14603-14611.
20. Yavorsky, D., Blanck, R., Lambalot, C., Brunkow, R. 2003. The clarification of bioreactor cell cultures for biopharmaceuticals. Pharmaceutical Technology 27(3): 62-76.83.