Gene Expression Profiles in Giant Freshwater Prawn Macrobrachium rosenbergii Infected with Macrobrachium rosenbergii Nodavirus (MrNV) by DD-PCR Technique
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Keywords:
Differential display PCR Differentially expressed genes Macrobrachium rosenbergii Real-time PCR MrNV
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Abstract
Differential Display PCR technique (DD-PCR) was used for analysis of altered gene expression in Macrobrachium rosenbergii infected with Macrobrachium rosenbergii Nodavirus (MrNV). Twenty-four combinations of arbitrary and oligo-dT primers were used to screen for differentially expressed genes. A total of 115 differentially expressed bands could be identified from 18 primer combinations. These included 67 bands (77%) whose expression levels increased and 48 bands (33%) which decreased in expression after MrNV challenge. Fifteen differential display fragments were successfully re-amplified, cloned and sequenced with 15 clones, 5 matched with sequences in the GenBank database. There were genes with known function, including Crustacyanin-like lipocalin, Ferritin 2 and dLp/HDL-BGBP. The other two genes were Ribosomal protein S2-like isoform 3 and Ribosomal protein L8. Crustacyanin-like lipocalin and ferritin 2 were selected for further confirmation for their differentially expression patterns by Quantitative Real-time PCR. The results indicated that these genes up regulated M. rosenbergii upon MrNV challenge. Crustacyanin-like lipocalin and Ferritin 2 were involved in shrimp defense immunity.
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Chungjit, A., Wuthisuthimethavee, S., Poompuang, S., & Tunkijjanukij, S. (2016). Gene Expression Profiles in Giant Freshwater Prawn Macrobrachium rosenbergii Infected with Macrobrachium rosenbergii Nodavirus (MrNV) by DD-PCR Technique. Journal of Fisheries and Environment, 40(2), 39–50. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/80921
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References
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26. Tung, C.W., C.S. Wang and S.N. Chen.1999. Histological and electron microscopic study on Macrobrachium muscle virus (MMV) infection in the giant freshwater prawn, Macrobrachium rosenbergii (de Man) culture in Taiwan. Journal of Fish Diseases 22:319-323.
27. Van Landingham, J.W. and C.W. Levenson. 2003. Effect of retinoic acid on ferritin H expression during brain development and neuronal differentiation. Nutritional neuroscience 6: 39-45.
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30. Xie, Y., F. Li, B. Wang, S. Li, D. Wang, H. Jiang, C. Zhang, K. Yu and J. Xiang. 2010. Screening of genes related to ovary development in Chinese shrimp Fenneropenaeus chinensis by suppression subtractive hybridization. Comparative Biochemistry and Physiology 5: 98-104.
31. Ye T., X. Wu, W. Wu, C. Dai, J. Yuan. 2015. Ferritin protect shrimp Litopenaeus vannamei from WSSV infection by inhibiting virus replication. Fish & Shellfish Immunology 42: 138-143.
32. Yoganandhan, K., M. Leartvibhas, S. Sriwongpuk and C. Limsuwan. 2006. White tail disease of the giant freshwater prawn Macrobrachium rosenbergii in Thailand. Diseases of aquatic organisms 69: 255-258.
33. Youngcharoen, S., S. Senapin, T. Lertwimol, S. Longyant, P. Sithigorngul, T.W. Flegel, P. Chaivisuthangkura. 2015. Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis Fish & Shellfish Immunology 45: 534- 542.
34. Zhang, J., F. Li, Z. Wang, X. Zhang, Q. Zhou and J. Xiang. 2006. Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis. Journal of Biotechnology 125: 173-184.
35. Zhou, J., W. Wang, G. Ma, A. Wang, W. He, P. Wang, Y. Liu, J. Liu and R. Sun. 2008. Gene expression of ferritin in tissue of the Pacific white shrimp, Litopenaeus vannamei after exposure to pH stress. Aquaculture 275: 356- 360.
2. Anderson, I.and Brass, A. 1998. Searching DNA databases for similarities to DNA sequences: when is a match significance. Bioinformatic 14:349-356.
3. Arcier, J.M., F. Herman, D.V. Lightner, R. Redman, J. Mari and J.R. Bonami. 1999. A viral disease associated with mortalities in hatchery-reared postlarvae of the giant freshwater prawn Macrobrachium rosenbergii. Diseases of aquatic organisms 38: 177-181.
4. Arockiaraj, J., A.J. Gnanamb, D. Muthukrishnanc, M. K. Thirumalai, M. Pasupuleti, J. Miltonc and M. Kasi. 2013. Macrobrachium rosenbergii cathepsin L: Molecular characterization and gene expression in response to viral and bacterial infections. Microbiological Research 168: 569-579.
5. Astrofsky, K.M., M.M. Roux, K.R. Klimpel, J.G. Fox and A.K. Dhar. 2002. Isolation of differentially expressed genes from white spot virus (WSV) infected Pacific blue shrimp (Penaeus stylirostris). Archives of virology 147: 1799-1812.
6. Chevion, M., S. Leibowitz, N.N. Aye, O. Novogrodsky and A. Singer. 2008. Heart protection by ischemic preconditioning: a novel pathway initiated by iron and mediated by ferritin. Journal of molecular and cellular cardiology 45: 839-45.
7. Cianci, M., P.J. Rizkallah, A. Olczak, J. Raftery, N.E. Chayen, P.F. Zagalsky and J.R. Helliwell. 2001. Structure of lobster apocrustacyanin Al using softer X-rays. Acta Crystallographica Biological Crystallography 57: 1219-1229.
8. Fagutao, F.F., M. Yasuike, M.D. Santos, L. Ruangpan, K. Sangrunggruang, A. Tassanakajon, Y. Takahashi, R. Ueno, H. Kondo, I. Hirono and T. Aoki. 2009. Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid. Developmental and Comparative Immunology 33:1088-1092.
9. Feng, W.R., M. Zhang, Y. Q. Su, J. Wang, Y. T. Wang and Y. Mao. 2014. Identification and analysis of a Marsupenaeus japonicus ferritin that is regulated at the transcriptional level by WSSV infection.Gene 544: 184-190.
10. Harrison, P.M. and P. Arosio . 1996. The ferritins: molecular properties, iron storage function and cellular regulation. Biochimica et Biophysica Acta 1275: 161-203.
11. Levenson, C.W. and C.A. Fitch. 2000. Effect of altered thyroid hormone status on rat brain ferritin H and ferritin L mRNA during postnatal development. Brain research Developmental brain research 119:105-9.
12. Luo, T., X. Zhang, Z. Shao and X. Xu. 2003. PmAV, a novel gene involved in virus resistance of Shrimp Penaeus monodon. FEBS letters 551: 53-57.
13. Nayak, S., S.K. Singh, N. Ramaiah and R.A. Sreepada. 2010. Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon post-larvae. Fish & Shellfish Immunology 29: 544-549.
14. Orino, K., K. Eguchi, T. Nakayama, S. Yamamoto and K. Watanabe. 1997. Sequencing of cDNA clones that encode bovine ferritin H and L chains. Comparative Biochemistry and Physiology Biochemistry & Molecular Biology 118: 667-673.
15. Orino, K., L. Lehman, Y. Tsuji, H. Ayaki, S.V. Torti and F.M. Torti. 2001. Ferritin and the response to oxidative stress. The Biochemical Journal 357: 241-247.
16. Owens, L., K. La Fauce, K. Juntunen, O. Hayakijkosol and C. Zeng. 2009. Macrobrachium rosenbergii nodavirus disease(white tail disease) in Australia. Diseases of Aquatic Organisms 85: 175-180.
17. Pan, D., N. He, Z. Yang, H. Liu and X. Xu. 2005. Differential gene expression profile in hepatopancreas of WSSV resistant shrimp (Penaeus japonicus) by suppression subtractive hybridization. Developmental and Comparative Immunology 29: 103-112.
18. Pfaffl, M.W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic acids research 29: 45.
19. Qian, D., Z. Shi, S. Zhang, Z. Cao, W. Liu, L. Li, Y Xie, I. Cambournac and J.R. Bonami. 2003. Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii. Journal of Fish Diseases 26: 521-527.
20. Roeser, H., A. Jacobs and M. Worwood. 1980. Iron metabolism in biochemistry and medicine.New York: Academic Press 605-640.
21. Sahul Hameed, A.S., K. Yoganandhan, J. Widada and J.R. Bonami. 2004. Studies on the occurrence and RT-PCR detection of Macrobrachium rosenbelgii nodavirus and extra small virus-like particles associated with white tail disease of Macrobrachium in India. Aquaculture 238: 127-133.
22. Somboonwiwat, K., P. Supungul, V. Rimphanitchayakit, T. Aoki, I. Hirono and A. Tassanakajon. 2006. Differentially Expressed Genes in Hemocytes of Vibrio harveyi challenged Shrimp Penaeus monodon. Journal of Biochemistry and Molecular Biology 39: 26-36.
23. Srisuk, C., S. Longyant, S. Senapin, P. Sithigorngul and P. Chaivisuthangkura. 2014. Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii. Fish & Shellfish Immunology 36: 552-562.
24. Theil E.C. 1987. Ferritin structure gene regulation and cellular function in animals plant and microorganisms. Annu. Rev.Biochem. 56: 289-315.
25. Theil, E.C. 2003. Ferritin: at the crossroads of iron and oxygen metabolism. The Journal of Nutrition 133:1549-1553.
26. Tung, C.W., C.S. Wang and S.N. Chen.1999. Histological and electron microscopic study on Macrobrachium muscle virus (MMV) infection in the giant freshwater prawn, Macrobrachium rosenbergii (de Man) culture in Taiwan. Journal of Fish Diseases 22:319-323.
27. Van Landingham, J.W. and C.W. Levenson. 2003. Effect of retinoic acid on ferritin H expression during brain development and neuronal differentiation. Nutritional neuroscience 6: 39-45.
28. Wade, N.M., M. Anderson, M.J. Sellars, R.K. Turne, N.P. Preston and B.D. Glencross. 2012. Mechanisms of colour adaptation in the prawn Penaeus monodon. The Journal of Experimental Biology 215: 343-350.
29. Wang, M.R., X.J. Zhu, J.S. Yang, Z. M. Dai, K. Mahmood, F. Yang and W.J. Yang. 2007. Prawn lipocalin: Characteristics and expressional pattern in subepidermal adipose tissue during reproductive molting cycle. Comparative Biochemistry and Physiology 147: 222-229.
30. Xie, Y., F. Li, B. Wang, S. Li, D. Wang, H. Jiang, C. Zhang, K. Yu and J. Xiang. 2010. Screening of genes related to ovary development in Chinese shrimp Fenneropenaeus chinensis by suppression subtractive hybridization. Comparative Biochemistry and Physiology 5: 98-104.
31. Ye T., X. Wu, W. Wu, C. Dai, J. Yuan. 2015. Ferritin protect shrimp Litopenaeus vannamei from WSSV infection by inhibiting virus replication. Fish & Shellfish Immunology 42: 138-143.
32. Yoganandhan, K., M. Leartvibhas, S. Sriwongpuk and C. Limsuwan. 2006. White tail disease of the giant freshwater prawn Macrobrachium rosenbergii in Thailand. Diseases of aquatic organisms 69: 255-258.
33. Youngcharoen, S., S. Senapin, T. Lertwimol, S. Longyant, P. Sithigorngul, T.W. Flegel, P. Chaivisuthangkura. 2015. Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M. rosenbergii nodavirus reveals their roles in apoptosis Fish & Shellfish Immunology 45: 534- 542.
34. Zhang, J., F. Li, Z. Wang, X. Zhang, Q. Zhou and J. Xiang. 2006. Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis. Journal of Biotechnology 125: 173-184.
35. Zhou, J., W. Wang, G. Ma, A. Wang, W. He, P. Wang, Y. Liu, J. Liu and R. Sun. 2008. Gene expression of ferritin in tissue of the Pacific white shrimp, Litopenaeus vannamei after exposure to pH stress. Aquaculture 275: 356- 360.