Identification and Expression of Vitellogenin Gene in Polychaetes (Perinereis sp.)
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Published:
May 30, 2017
Keywords:
Polychaete Vitellogenin gene Real-time quantitative RT-PCR
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Abstract
Polychaetes (Perinereis sp.) have been used extensively in shrimp broodstock maturation diets because of their nutritional value, being rich in protein, fat and minerals. The aims of this study were to identify the reproduction-related gene in polychaetes, and analyze gene expression levels in polychaetes. Messenger RNA (mRNA) was isolated from mature polychaete samples and used as the template for complementary DNA (cDNA) synthesis. The cDNAwas then subjected to cloning and sequencing. However, no cDNA sequence was found to match the reproductive-related gene. As an alternative procedure, a set of cDNA was synthesized and sequenced by the pyrosequencing method. From 454 Pyrosequencing, 40,402 raw sequences were obtained, covering 14,747,824 nucleotides 200-300 bp inlength andgrouped into 609 isotigs, 712 contigs and 15,975 singletons. In addition, BLASTX analysis showed that 410 sequences (67.3%) matched known genes, whereas, 199 sequences (32.7%) showed no significant homology to known genes. A significant match was found with the vitellogenin structural genes' (yolk protein genes) family member (vit-1)-like gene, which was grouped as the reproduction-related gene in polychaetes. The gene was then used to analyze the expression levels inpolychaetes aged 2, 3, 4, 5, 6, 7, and 8 months by real-time quantitative RT-PCR. The highest vitellogenin gene expression was found in6 -month old polychaetes (P<0.05) and dramatically decreased after reaching 6 months. This result suggested that 6 -month old polychaetes will be suitable as the maturation diet for shrimp broodstock; however, the effect on shrimp reproductive development may need to be analyzed further.
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Phoonsamran, K., Direkbusarakom, S., Chotipuntu, P., Hirono, I., Unajak, S., Summpunn, P., & Wuthisuthimethavee, S. (2017). Identification and Expression of Vitellogenin Gene in Polychaetes (Perinereis sp.). Journal of Fisheries and Environment, 41(1), 1–11. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/87732
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References
1. Baert, J. L.and M.C. Slomianny. 1987. Heterosynlhetic origin of the major yolk protein vitellin, in a nereid Perinereis cultrifera. Comparative Biochemistry and Physiology Part B 88: 1191-1199.
2. Bray, W.A. and A. L.Lawrence. 1992. Reproduction of Penaeus Species in Captivity. In:Marine Shrimp Culture: Principle and Practices, A.W.Fast and L.J. Lester, editors. (pp. 93- 170). Amsterdam, The Netherlands: Elsevier.
3. Brown, N., S. Eddy and S.Plaud. 2011.Utilization of waste from amarine recirculating fish culture system as a feed source for the polychaete worm, Nereis virens. Aquaculture 322-323: 177-183.
4. Chimsung, N. 2014. Maturation diets for black tiger shrimp (Penaeus monodon) broodstock: a review. Songklanakarin Journal of Science and Technology 36 (3): 265-273.
5. Conesa, A. and S. Götz . 2008. Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genornics. International Journal of Plant Genomics: 1-13.
6. Darya, M., M. Sajjadi and I. Sourinejad. 2016. Determination of fecundity,weight-fecundity relationship, oocyte diameter and sex ratio of polychaete Perinereis nuntia in the coastal waters of Persian Gulf (Bandar Abbas, Iran). Journal of Aquaculture Development, 10 (3):0-0. URL:http://aqudev.liau.ac.ir/article-1-408-en.html
7. D, Croz, L., L. Wong, G.Justines and M. Gupta. 1988. Prostaglandins and related compounds from the Polychaete Worm Americonuphis reesei Fauchald (Onuphidae) as possible inducer of gonad maturation in penaeid shrimps. Revista de Biologia Tropical 36 (1): 331- 332.
8. Delcher, A. L., K. A. Bratke, E. C. Powers and S. L. Salzberg. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23 (6): 673-679.
9. Dhainaut, A. 1984. Oogenesis in polychaetes. Ultrastructural differentiation andmetabolism of nereid oocytes. Fortschr.ZooL 29: 183-204.
10. Fischer, A. and A. Dhainaut. 1985. The origin of yolk in the oocytes of Nereis virens (Annelida Polychaeta). Cell and Tissue Research 240: 67-76.
11. Fischer, A. and H. Rabien. 1986. Molecular and cellular functions driving oocyte growth in nereid. Advanced Invertebrate Reproduction 4: 195-205.
12. Fischer, A., H. Rabien and A. E. Heacox. 1991. Specific, concentration-dependent uptake of vitellin by the oocytes of Nereis virens (Annelida Polychaeta) in vitro. Journal of Experimental Zoology 260: 106-115.
13. Fischer, A. and U. Hoeger. 1993. Metabolic links between somatic sexual maturation and oogenesis innereid annelids-a brief review. Invertebrate Reproduction and Development 23: 131-138.
14. Fischer,A., A. W.C. Dorresteijn and U. Hoeger. 1996. Metabolism of oocyte construction and the generation of histospecificity in the cleaving egg. Lessons from nereid annelids. The International Journal of Developmental Biology 40: 421-430.
15. Garc1'a-Alonso, J., U. Hoeger and N. Rebscher. 2006. Regulation of vitellogenesis in Nereis virens (Annelida: Polychaeta): Effect of estradiol-17h on eleocyte Comparative Biochemistry and Physiology Part A 143: 55-61.
16. Hafer, J., A. Fischer and H. J. Ferenz. 1992. Identification of the yolk receptor protein in oocytes of Nereis virens (Annelida, Polychaeta) and comparison with the locust vitellogenin receptor. Journal of Comparative Physiology B 162: 148-152.
17. Hardege, J. D., H. D. Bartels-Hardege, Y. Yang, B. L. Wu, M. Y. Zhu and E. Zeeck. 1994. Environmental control of reproduction in Perinereis nuntia Var. brevicirrus. Journal of the Marine Biological Association UK 74: 903-918.
18. Harrison, K.. 1990. The role of nutrition in maturation, reproduction and embryonic development of decapods crustaceans, a review. Journal of Shellfish Research 9: 1-28.
19. Harrison, K. 1997. Broodstock nutrition and maturation diets. In Crustacean Nutrition, Advances in World Aquaculture,
20. L.R. D'Abramo, D.E. Conklin, and D.M.Akiyama, editors, World Aquaculture Society, (pp. 390-408). Louisiana, U.S.A., BatonRouge.
21. Koskela, R W.,J. G.Greenwood and P.C.Rothlisberg. 1992. The influence of prostaglandins E2 and 1hesteroidhormones, 17α-hydroxyprogesteron development the tiger prawn, Penaeus esculentus. Comparative Biochemistry and Physiology l0l(A): 295-299.
22. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data usin real time quantitative PCR and the 2-∆∆CT method. Methods 25: 402-408.
23. Laufer, H., D. Borst, F. C. Baker, C. Carrasco, M. Sinkus, C.C.Reuter, ... D. S. Schooley. 1987. Identification-of a juvenile hormone-like compound in a crustacean. Science 235 (4785): 202-205.
24. Lawrence, A. J. and J.M. Soame. 2009.The endocrine control of reproduction in Nereidae: a new multi-hormonal model with implications for their functional role in a changing environment. Philosophical Transactions of the Royal Society B 364: 3363-3376.
25. Lee, R,A. Walker andD.Reish. 2005.Charncterization of lipovitellin in eggs of the polychaete Neanthes arenaceodentata. Comparative Biochemistry and Physiology Part B 140: 381-386.
26. Meunpol, O., P. Meejing and S. Piyatiratitivorakul. 2005. Maturation diet based on fatty acid content for male Penaeus monodon (Fabricius) broodstock. Aquaculture Research 36: 1216-1225.
27. Meunpol, O., S. lam-Pai, W. Suthikrai and S. Piyatiratitivorakul. 2007. Identification of progesterone and l7α-hydroxyprogesterone inpolychaetes (Perinereis sp.) and the effects of hormone extracts on penaeid oocyte development in vitro. Aquaculture 270: 485-492.
28. Meunpol, O., E. Dangjai, R. Yoonpum and S. Piyatiratitivorakul. 2010. Detection of prostaglandin E2 in polychaete Perinereis sp. And its effect on Penaeus monodon oocyte development in vitro. Fisheries Science 76: 281-286.
29. Olive, P.J. w., A Grant and P.D. Cowin. 1986. Decision and choice in Polychaete reproductive cycles. In Prochet M, Anmies J.-C., Dhaioaut A (&ls), Advances in invertebrate reproduction (4th ed., pp. 371-378). Amsterdam, The Netherlands: Elsevier.
30. Olive, P. J. W., S. W. Rees and A. Djunaedi. 1998. Influence of photoperiod and temperature on oocyte growth in the semelparous polychaete Nereis (Neanthes) virens. Marine Ecology Progress Series 172: 169-183.
31. Palmer, P. J. 2011. Commercial application of polychaete sand filters for wastewater remediation and broodstock feeds. In Technical Report (p l-34). Queensland, Australia: Bribie Island Research Centre.
32. Pinon, E. 2000. Producing ragworms for shrimp broodstock matmation. Global Aquaculture Advocate 3: 82-84.
33. Poltana, P., T. Lerkitkul, P. Pongtippatee-Taweepreda, S. Asuvapongpattana, K. Wongprasert, S. Sriurairatana, J. Chavadej, P. Sobhon, P.J.W. Olive and B. Withyachumnarnkul. 2007. Culture and development of polychaete Perinereis cf. nuntia. Invertebrate Reproduction and Development 50: 13-20.
34. Rees, S.W. and P. J. W. Olive. 1999. Photoperiodic changes influence the incorporation of vitellin yolk protein by oocytes of the semelparous polychaete Nereis (Neanthes) virens. Comparative Biochemistry and Physiology Part A 123: 213-220.
35. Sukcharoen, K., J. Chiayvareesajja and P. Poltana. 2011. Growth rate of polychaete (Perinereis nuntia). Valaya Alongkorn Review 1 (2): 93-101.
36. Wouters, R, P.Lavens, J. Nieto and P. Sorgeloos. 2001. Penaeid shrimp broodstock nutrition: an updated review on research and development Aquaculture 202: 1-21
2. Bray, W.A. and A. L.Lawrence. 1992. Reproduction of Penaeus Species in Captivity. In:Marine Shrimp Culture: Principle and Practices, A.W.Fast and L.J. Lester, editors. (pp. 93- 170). Amsterdam, The Netherlands: Elsevier.
3. Brown, N., S. Eddy and S.Plaud. 2011.Utilization of waste from amarine recirculating fish culture system as a feed source for the polychaete worm, Nereis virens. Aquaculture 322-323: 177-183.
4. Chimsung, N. 2014. Maturation diets for black tiger shrimp (Penaeus monodon) broodstock: a review. Songklanakarin Journal of Science and Technology 36 (3): 265-273.
5. Conesa, A. and S. Götz . 2008. Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genornics. International Journal of Plant Genomics: 1-13.
6. Darya, M., M. Sajjadi and I. Sourinejad. 2016. Determination of fecundity,weight-fecundity relationship, oocyte diameter and sex ratio of polychaete Perinereis nuntia in the coastal waters of Persian Gulf (Bandar Abbas, Iran). Journal of Aquaculture Development, 10 (3):0-0. URL:http://aqudev.liau.ac.ir/article-1-408-en.html
7. D, Croz, L., L. Wong, G.Justines and M. Gupta. 1988. Prostaglandins and related compounds from the Polychaete Worm Americonuphis reesei Fauchald (Onuphidae) as possible inducer of gonad maturation in penaeid shrimps. Revista de Biologia Tropical 36 (1): 331- 332.
8. Delcher, A. L., K. A. Bratke, E. C. Powers and S. L. Salzberg. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23 (6): 673-679.
9. Dhainaut, A. 1984. Oogenesis in polychaetes. Ultrastructural differentiation andmetabolism of nereid oocytes. Fortschr.ZooL 29: 183-204.
10. Fischer, A. and A. Dhainaut. 1985. The origin of yolk in the oocytes of Nereis virens (Annelida Polychaeta). Cell and Tissue Research 240: 67-76.
11. Fischer, A. and H. Rabien. 1986. Molecular and cellular functions driving oocyte growth in nereid. Advanced Invertebrate Reproduction 4: 195-205.
12. Fischer, A., H. Rabien and A. E. Heacox. 1991. Specific, concentration-dependent uptake of vitellin by the oocytes of Nereis virens (Annelida Polychaeta) in vitro. Journal of Experimental Zoology 260: 106-115.
13. Fischer, A. and U. Hoeger. 1993. Metabolic links between somatic sexual maturation and oogenesis innereid annelids-a brief review. Invertebrate Reproduction and Development 23: 131-138.
14. Fischer,A., A. W.C. Dorresteijn and U. Hoeger. 1996. Metabolism of oocyte construction and the generation of histospecificity in the cleaving egg. Lessons from nereid annelids. The International Journal of Developmental Biology 40: 421-430.
15. Garc1'a-Alonso, J., U. Hoeger and N. Rebscher. 2006. Regulation of vitellogenesis in Nereis virens (Annelida: Polychaeta): Effect of estradiol-17h on eleocyte Comparative Biochemistry and Physiology Part A 143: 55-61.
16. Hafer, J., A. Fischer and H. J. Ferenz. 1992. Identification of the yolk receptor protein in oocytes of Nereis virens (Annelida, Polychaeta) and comparison with the locust vitellogenin receptor. Journal of Comparative Physiology B 162: 148-152.
17. Hardege, J. D., H. D. Bartels-Hardege, Y. Yang, B. L. Wu, M. Y. Zhu and E. Zeeck. 1994. Environmental control of reproduction in Perinereis nuntia Var. brevicirrus. Journal of the Marine Biological Association UK 74: 903-918.
18. Harrison, K.. 1990. The role of nutrition in maturation, reproduction and embryonic development of decapods crustaceans, a review. Journal of Shellfish Research 9: 1-28.
19. Harrison, K. 1997. Broodstock nutrition and maturation diets. In Crustacean Nutrition, Advances in World Aquaculture,
20. L.R. D'Abramo, D.E. Conklin, and D.M.Akiyama, editors, World Aquaculture Society, (pp. 390-408). Louisiana, U.S.A., BatonRouge.
21. Koskela, R W.,J. G.Greenwood and P.C.Rothlisberg. 1992. The influence of prostaglandins E2 and 1hesteroidhormones, 17α-hydroxyprogesteron development the tiger prawn, Penaeus esculentus. Comparative Biochemistry and Physiology l0l(A): 295-299.
22. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data usin real time quantitative PCR and the 2-∆∆CT method. Methods 25: 402-408.
23. Laufer, H., D. Borst, F. C. Baker, C. Carrasco, M. Sinkus, C.C.Reuter, ... D. S. Schooley. 1987. Identification-of a juvenile hormone-like compound in a crustacean. Science 235 (4785): 202-205.
24. Lawrence, A. J. and J.M. Soame. 2009.The endocrine control of reproduction in Nereidae: a new multi-hormonal model with implications for their functional role in a changing environment. Philosophical Transactions of the Royal Society B 364: 3363-3376.
25. Lee, R,A. Walker andD.Reish. 2005.Charncterization of lipovitellin in eggs of the polychaete Neanthes arenaceodentata. Comparative Biochemistry and Physiology Part B 140: 381-386.
26. Meunpol, O., P. Meejing and S. Piyatiratitivorakul. 2005. Maturation diet based on fatty acid content for male Penaeus monodon (Fabricius) broodstock. Aquaculture Research 36: 1216-1225.
27. Meunpol, O., S. lam-Pai, W. Suthikrai and S. Piyatiratitivorakul. 2007. Identification of progesterone and l7α-hydroxyprogesterone inpolychaetes (Perinereis sp.) and the effects of hormone extracts on penaeid oocyte development in vitro. Aquaculture 270: 485-492.
28. Meunpol, O., E. Dangjai, R. Yoonpum and S. Piyatiratitivorakul. 2010. Detection of prostaglandin E2 in polychaete Perinereis sp. And its effect on Penaeus monodon oocyte development in vitro. Fisheries Science 76: 281-286.
29. Olive, P.J. w., A Grant and P.D. Cowin. 1986. Decision and choice in Polychaete reproductive cycles. In Prochet M, Anmies J.-C., Dhaioaut A (&ls), Advances in invertebrate reproduction (4th ed., pp. 371-378). Amsterdam, The Netherlands: Elsevier.
30. Olive, P. J. W., S. W. Rees and A. Djunaedi. 1998. Influence of photoperiod and temperature on oocyte growth in the semelparous polychaete Nereis (Neanthes) virens. Marine Ecology Progress Series 172: 169-183.
31. Palmer, P. J. 2011. Commercial application of polychaete sand filters for wastewater remediation and broodstock feeds. In Technical Report (p l-34). Queensland, Australia: Bribie Island Research Centre.
32. Pinon, E. 2000. Producing ragworms for shrimp broodstock matmation. Global Aquaculture Advocate 3: 82-84.
33. Poltana, P., T. Lerkitkul, P. Pongtippatee-Taweepreda, S. Asuvapongpattana, K. Wongprasert, S. Sriurairatana, J. Chavadej, P. Sobhon, P.J.W. Olive and B. Withyachumnarnkul. 2007. Culture and development of polychaete Perinereis cf. nuntia. Invertebrate Reproduction and Development 50: 13-20.
34. Rees, S.W. and P. J. W. Olive. 1999. Photoperiodic changes influence the incorporation of vitellin yolk protein by oocytes of the semelparous polychaete Nereis (Neanthes) virens. Comparative Biochemistry and Physiology Part A 123: 213-220.
35. Sukcharoen, K., J. Chiayvareesajja and P. Poltana. 2011. Growth rate of polychaete (Perinereis nuntia). Valaya Alongkorn Review 1 (2): 93-101.
36. Wouters, R, P.Lavens, J. Nieto and P. Sorgeloos. 2001. Penaeid shrimp broodstock nutrition: an updated review on research and development Aquaculture 202: 1-21