Identification and Characterization of Potential Probiotic Bacillus spp. for Application in Striped Catfish (Pangasianodon hypophthalmus [Sauvage, 1878])
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Jul 15, 2017
Keywords:
Isolation characterization probiotics Bacillus striped catfish Vietnam
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Abstract
One hudred and twenty isolates of Bacillus sp. were collected from the intestines of striped catfish (Pangasianodon hypophthalmus [Sauvage, 1878]) reared in farms in three provinces in the southern part of Vietnam: An Giang, Can Tho and Dong Thap. Isolates were identified by multiplex and single PCR using specific primers designed on gene 16S rRNA for six species of Bacillus (B.Subtilis, B. amyloliguefaciens, B. cereus, B. megaterium, B. licheniformis and B. pumilus). The in vitro analysis included inhibition activity against the pathogenic bacterium Edwardsiella ictaluri, protesase activity, stomach acidity and bile tolerance test, and antibiotic susceptibility. Results indicated that the highest number of isolate was B. megaterium and none was identified as B. licheniformis. The strain B. amyloliquefaciens 54A showed high protease activity and B. pumilus 47B showed high ingibition activity against E. ictaluri. These two probiotic candidates were also resistant to stomach acid pH and low bile salt conditions after 3 h and 24 h of exposure. Bacillus sp. candidates displayed varying sensitivities to the tested antibiotics with the exception of spectinomycin.
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Truong Thy, H. T., Thinh, N. H., Tri, N. N., Quy, O. M., Kannika, K., Unajak, S., & Areechon, N. (2017). Identification and Characterization of Potential Probiotic Bacillus spp. for Application in Striped Catfish (Pangasianodon hypophthalmus [Sauvage, 1878]). Journal of Fisheries and Environment, 41(2), 20–36. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/92748
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References
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35. Hong, H.A., L.H. Due and S.M. Cutting. 2005. The use bacterial spore formers as probiotics. FEMS Microbiol. Rev. 29: 813-835.
36. Hyronimus, B., C. Le Marrec, A.H. Sassi and A. Deschamps. 2000. Acid and bile tolerance of spore-forming lactic acid bacteria. Int. J. Food Microbiol. 61: 193-197.
37. Jeney, G., T.T. Dung, D.T.H. Oanh, Z. Jeney and N. A. Tuan. 1998. Preliminary results of the baseline survey on fish health management in freshwater aquaculture of the Mekong Deltaof Vietnam. J.Appl Ichthyol. 14: 223.
38. John, E.H. 2011. Guyton and Hall Textbook of Medical Physiology.U.S.: Saunders Elsevier
39. Jose, L.B.Z., LD. Blas, R.Z. Imanol, C. David, V. Daniel, L.M.Z. Jose. 2006. The role of probiotics in aquaculture. Vet. Microbiol. 114: 173-186.
40. Kawasaki, H., Y. Hoshino, A. Hirata and IC. Yamasato. 1993. Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. Arch. Microbiol. 160: 358-362.
41. Kim, T.W., Y.H. Kim, S.E. Kim, J.H. Lee, C.S. Paik and H.Y. Kim. 2010. Identification and distribution of Bacillus species in doenjang by whole-cell protein patterns and l6S rRNA gene sequence analysis. J. Microbiol. Biotechnol. 20: 1210-1214.
42. Kwon, G.H., H.A. Lee, J.Y. Park, J.S. Kim, J. Lim, C.S. Park, D.Y. Kwon, Y.S. Kim and J.H. Kim. 2009. Development of RAPD-PCR method for identification of Bacillus species isolated from Cheonhhukjang. Int. J.Food Microbiol. 129: 282-287.
43. Leyton, Y.E., R.A. Varas Psija and C.E. Riquelme.2012. Probiotic activity of bacteria associated with egg capsules of Concholepas (commonname 'Loco'). Aquac. Res. 43: 1089-1095.
44. Liong, M.T. and N.P. Shah. 2005. Acid and bile tolerance and cholesterol removal ability of Lactobacilli strains. J Dairy Sci. 88: 55-66.
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