Effects of Aquanin Plus (Beta-Cyclodextrin Cysteamine Hydrochloride) on the Growth,Survival and Immune Characteristics of Pacific White Shrimp (Litopenaeus vannamei)

Abstract

A study of the effects of Aquanin plus (beta-cyclodextrin cysteamine hydrochloride) on the growth, survival and immune response in Pacific white shrimp (Litopenaeus vannamei) was conducted under laboratory conditions. For the determination of the growth-promotiong and immunostimulant effects of Aquanin plus adiministration in the diet,tests were carried out in three treatments (with six replicates per treatment). Enach replicate consisted of 25 shrimp (ๅ11-12 g) in 500-liter tanks. Shrimp were fed three times daily at 3% body weight per day for 60 day with pelleted feed containing graded levels of Aquanin plus (0, 0.5 and 0.1% of the feed). After 40 days of dietary administration, shrimp fed with 0.1% Aquanin plus had an average body weight (16.76±1.33 g) significantly higher (P<0.05) than that of 0.05 % Aquanin plus (15.84±0.99 g) and control groups (15.40±1.19 g). There was no significant difference in survival between the two Aquanin plus-treated groups (97.18-97.60%) but their wurvival rates were significantly higher (P<0.05) than those of the control group (83.20%). The shrimp immune responses were measured by total hemocyte count (THC), percentage phagocytosis, phenoloxidase activity and bactericidal activity. The results showed that shrimp which consumed diets containing 0.1% Aquanin plus had significantly higher (P<0.05) THC, percentage phagocytosis and phenoloxidase activity than those of the 0.05% Aquanin plus and control groups. Shrimp fed with 0.05 and 0.1% Aquanin plus had bactericidal activity at the serum dilution of 1:16 while the control group was 1:8. In conclusion, the present study indicated that oral administration of 0.1% Aquanin plus 40 days could increase the growth, survival and immune response of L.vannamei.