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Periodontal diseases are an inflammatory diseases of periodontal tissue. The 6th epidemiological survey of this disease in Thailand during the year 2006-2007 reported that most of the elderly have periodontitis. The cause of periodontal disease is bacteria, which induces infections by plaque formation and cause inflammatory. Periodontal disease can be detected by many methods such as periodontal probing depth, bacterial culture, enzyme-linked immunosorbent assay (ELISA), and immunoassay of saliva and gingival crevicular fluid (CGF) specimens. The biological markers in CGF including myeloperoxidase (MPO) which is an enzyme found in azurophilic granule of neutrophil. When host response to injuries, neutrophil release MPO to catalyze halogenation reaction. Hypochlorous acid, the end product of this reaction, is toxic to bacteria. The color product obtained from MPO catalyzed selected substrate can be easily detect by eye or colorimetry. Thus MPO may be a potential enzyme for development of diagnostic kit for screening periodontal disease.