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The microbiological culture techniques were utilized to isolate microorganisms in peritoneal effluent samples collected from 45 continuous ambulatory peritoneal dialysis (CAPD) patients at Srinagarind Hospital. Almost 9 percent (38 of 427) were positive cultures for microorganisms. Thirty-five bacterial and 11 fungal isolates were detected. The most common bacteria among the gram positive cocci was coagulase negative Staphylococcus. The less commonly recovered organisms were Sterptococcus group D enterococci and Streptococcus other than group D. Among gram negative bacilli, Enterobacter sp. Was the most frequently isolated. Pseudomonas aeruginosa, Acinetobacter calcoaceticus var anitratus, Klebsiella pneumoniae and Bacteroides sp. Were less cmmonly observed. Trichosporon inkin was the most frequently found among the fungal organisms followed by yeast cells and Candida rugosa. Antimicrobial susceptibility patterns of the bacteria isolated are as follow : coagulase negative Staphylococcus was 89% sensitive to cephalothin, but only 25% to gentamicin.