In-vitro evaluation of antiseptics and disinfectants against agianst Burkholderia pseudomallei from clinical isolates.

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Thitima Chaita
Krittiya Wanitjeiwaphan
Pleonphit Hannond
Arunya Kongthaworn
Wises Namwat
Sunpetch Angkititrakul
Thicumporn Kuyyakanond



Objective: Chemical widely used as antiseptics and disinfectants for killing various microorganisms in many studies whether no evaluation of chemical agents against

B. pseudomallei .

Materials: Our study performed  various concentrations of common used as antiseptics and disinfectants in hospitals for example phenol derivatives, alcohol, halogen compounds, aldehyde and quaternary ammonium compounds against B. pseudomallei. These informations will help physician who work in infectious unit easy choose these agents for killing or controlling spread in hospital environment especially  these organisms can survive in water or humid area as Psedomonas aeruginosa.

Results: This study using chemical agents in clinic performed in-vitro evaluation on B. pseudomallei from various specimens of Srinagarind hospital by dilution method.  This found that good antiseptics against these organisms were 70%Alcohol 2%ProvidineÒ 0.1%ThimersalÒ and 2% MecurochromeÒ; good disinfectants against that were 4%Formaldehyde, 2%LysolÒ and 3%H2O2. The 0.5% HibitaneÒ has modertaedly  antibactericidal effect on B. pseudomallei . Both 1:100 SavlonÒ and 0.1%AcriflavinÒ were poor antibactericidal against these organisms. Whereas 1% VirkonÒ was optimum concentration for killing these organisms and against contaminations not more than

107 cells/ml.

Conclusions: The effective chemical agents against  B. pseudomallei were  0.1-0.5% Chlorine, 4%Foramldehyde, 3%H2O2, 2%LysolÒ, 70%Alcohol, 2%ProvidineÒ, 0.1%ThimerosalÒ and 2% MecurochromeÒ

Keywords: antiseptics, disinfectants, Burkholderia pseudomallei, clinical isolates


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