Partial Purification of Urinary Erythropoietin from Anemic Patients

Abstract

ABSTRACT

Urinary erythropoietin from the anemic patients of Chiang Mai Hospital, Chiang Mai University, Chiang Mai, Thailand was prepared and partially purified using various types of colum chromatographic techniques; DEAE-Cellulose, Hydroxyapatite, Sephadex G-25 and Sephadex G-100 Columns.  From this investigation, it was found that the actual yield obtained from 30 litres of original urine volume was 65 milligrams, the erythropoietic activity was 686.80 Cobalt Units per milligram and the purification factor was 6,733 times comparing with the Crude Urinary Powder.

Key Words : Erythropoietin, anemic patients, column chromatography, erythropoietic activity, purification factor.