Anti-Sm and Anti-RNP Detection by Passive Hemagglutination and Counter Immunoelectrophoresis

Abstract

AbstractA comparison study of anti-Som and anti-RNP detection between Passive hemagglutionation (PHA) and Counter immunoelectrophoresis (CIE) in 92 autoimmune disease sera, included 52 sera from patient with SLE, 18 sera from patient with MCTD and 22 sera from patients with other diseases.  The methodology of this study was blind assesed through out the studies.  The results of PHA detection showed that Patients with SLE 17 cases were positive for anti-Sm, 10 cases were both positive for anti-Sm and anti-RNP and 25 cases were negative.  Patients with MCTD 14 cases were positive for anti-RNP and 4 cases were negative whereas the other disease patients were all negative.  The results of CIE detection in the same patient groups were found that patients with SLE 34 cases were positive for anti-Sm, 6 cases were both positive for anti-Sm and anti-RNP and 12 cases were negative.  Patients with MCTD 15 cases were positive for anti-RNP and 3 cases were negative whereas the other disease patients 20 cases were negative and only 2 cases were positive for anti-RNP

Consideration in the detection of anti-Sm and anti-RNP by CIF were more sensitive than PHA in both groups of patients with SLE (76.92% and 51.92%; respectively) and MCTD (83.33% and 77.78%; respectively).  More over the detection by CIE and PHA gave no difference in specificity in both groups of patients with SLE (100% equaly for two methods) and MCTD (97.30% and 100%; respectively).  The correlation between these two methods were good agreement with statistical significance in both groups of patients with SLE (k=0.6296;p<0.001) and MCTD (k=0.7299;p<0.001).