Enterotoxins, TSST-1 Production and Drug Semsitivity Of Staphyrococcus aureus Isolated from Hospital Staffs And Medical Student in Srinagarind Hospital

Authors

  • Chariya Chomvarin
  • Sukanya Srigulbutr
  • Sorujsiri Chareonsudjai
  • Junpen Bourporn

Abstract

Background : Staphylococus  aureus  distribution  is  associated  with  the  nasal  and  hand  carriage  in  hospital  staff and  medical  students. A factor  associated  with  the  virulence  of  disease  is  the  ability  of  bacteria  to  produce  the  enterotoxins (Se)  and  Toxic  shock  syndrome  toxin – 1 (TSST -1). A problem  in  treatment  was  an  increase  in  drug  resistance  particularly,  resistance  to  methicillin  grourp  (MRSA).

Objectives : To  investigate and  to  compare  the  prevalence  of  nasal  and  hand  carriage  of  Staphylococcus  aureus  in  the  hospital  staffs  and  the  medical  students  studying  in  the  preclinical  class  (the  third  year)  and  in  the  clinical  class  (the  fifth  year)  The  occurrence  of  enterotoxin  and  TSST-1  production  of  Staphylococcus  aureus  isolated  were  examined.  Moreover,  drug  resistance  was  also  evaluated.

Design : Survey  and  laboratory  study.

Setting : At  Srinagarind  hospital,  Faculty  of  medicine,  KKU

Subjects : One  hundred  and  eight  hospital  staff (phyusicians,  nurses,  workers)  and  63  medical  students  (during  their  studies  in  preclinic  and  in  clinic  classes) were  examined.

Methods : Staphylococcus  aureus  were  identiflied  by  coagulase  test  and  sugar  fermentation.  The  enterotoxin and  TSST-1 production  were  detected  by  reersed  passive  latex  agglutination.  Drug  resistance  was  tested  by  disk  agar  diffusion  method.

Results: No  significant  difference  in  the  prevalence  of  nasal  and  hand  carriage  of  Staphylococcus  aureus  was  found  between  the  hospital  staff  and  the  medical  students  in  both years (55.6%-59.3% in  anterior  nares  and  38.1%-44.4% in hands). MRSA  was  found  in  anterior  nares  and  hands  of  the  medical  staff  with  10.2%  and 9.3%  respectively.  Those  of  the  third  year  and  the  fifth  year  were  found  with  1.6%, 0%  and  3.2%,  1.6%  respectively. The  enterotoxin  and  TSST-1 production  occurred  in  13.9%  of  anterior  nares  and  5.6%  of  hands  of  the  hospital  staff  and  were  found  more  frequently  in  MSSA  than  in  MRSA.  The  SeA  was  the  most  predominant  enterotoxin  isolated  from  both  organs  of  the  medical  staff  and  was  found  in  only  the  fifth  year  medical  students.  With  the  increasing  clinical  exposure  (the fifth  year),  the more  enterotoxins  were  found.  The  production  of  enterotoxins  were  found  in  14.3%  of  anterior  nares  and  4.7%  of  hands  of  the  fifth  year  medical  students  or  20.5%  and  11.5%  of  total  Staphylococcus  aureus  isolation  whereas  they  were  found  in  7.9%  of  anterior  nares  and  4.7%  of  hands  of  the  third  year  medical  students  or  11.9%  and  9.7  of  total  Staphylococcus  aureus isolation. TSST-1 was  also found in strains  isolated  from both  organs  of  the  hospital  staff  but was  not  found  in the  medical  students.  Resistance  to  penicillin,  tetracycline, lincomycin occurred more  frequent  respectively in  strains  isolated  from both the  hospital  staff  and the  medical  students.

Conclusions : No significant difference  of  carriage  of  Staphylococcus  aureus  was  found  in  the  hospital  staff  and  the  medical  students.  Drug  resistance was  increased with  increasing  clinical  exposure.  The enterotoxin and  TSST-1 productions  and the  type  of  enterotoxin  combination  were  found  more  frequently in  clinical  exposed  people  than  non  clinical  exposed  people although  there  was  no  significant difference.

Keywords : Staphylococcus  aureus, enterotoxin, TSST-1

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Chomvarin C, Srigulbutr S, Chareonsudjai S, Bourporn J. Enterotoxins, TSST-1 Production and Drug Semsitivity Of Staphyrococcus aureus Isolated from Hospital Staffs And Medical Student in Srinagarind Hospital. SRIMEDJ [Internet]. 2013 Nov. 20 [cited 2024 Oct. 8];12(4):217-2. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/14009

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